ANTIMICROBIAL AND STRUCTURAL EFFECTS OF DIFFERENT IRRIGATION SOLUTIONS ON GUTTA-PERCHA CONES

Abstract

Purpose: To evaluate the alterations on the surface of gutta-percha cones (GPCs) on exposure to the different irrigation solutions and their possible antibacterial effect against Enterococcus faecalis. (E. faecalis)
Materials and Methods: Disinfection ability of different solutions (5.25% sodium hypochlorite, 2% chlorhexidine, 1% peracetic acid, and QMix) were tested with 96 GPCs and the time of exposure to each solution was 5 and10 minutes, respectively. GPCs used in this study were contaminated with E.faecalis. After disinfection, GPCs were placed in tubes containing the medium and incubated at 37˚C for 7 days. All tubes were visually checked for turbidity at 24-hour intervals. About 92 new GPCs were analyzed by means of SEM/EDS to assess the topography and chemical elements present on their surface. The data generated was analyzed using Pearson chi-square test, p<0.05.
Results: There were no significant statistical differences in disinfection quality between the irrigation solutions used on GPCs contaminated with E. faecalis (p>0.05). SEM/EDS analyses showed no alteration in the superficial features of GPCs after treating with various irrigation solutions.
Conclusion: QMix was found to be an effective agent for rapid disinfection of GPCs as well-known irrigation solutions. Irrigation solutions were found to have sterilized the GPCs after both 5 and 10 minutes of exposure.

Keywords

• Antimicrobial agents; Energy Dispersive X-ray Spectroscopy; Gutta-Percha cones

Farklı Kök Kanalı Yıkama Solüsyonlarının Gütaperka Üzerindeki Yapısal ve Antimikrobiyal Etkileri

Abstract

Amaç : Farklı kök kanal yıkama solüsyonlarının Enterococcus faecalis (E. faecalis) ile kontamine olan gütaperka konları (GPK) üzerindeki antibakteriyel ve yapısal etkilerinin incelenmesidir. Gereç ve Yöntem: Antimikrobiyal etkinliğin tespitinde 96 adet GPK kullanılmıştır. GPK, E.faecalis ile kontamine edildikten sonra, % 5.25’lik sodyum hipoklorit,% 2’lik klorheksidin,% 1’lik perasetik asit ve QMix solüsyonlarında 5 ve 10 dakika boyunca bekletilmişlerdir. Bu sürenin sonunda GPK besi ortamı içeren tüplere konularak 7 gün süreyle 37°C’de inkübe edilmiştir. Tüpler 24 saat arayla bulanıklık açısından kontrol edilerek bulgular kaydedilmiştir. Solüsyonların GPK üzerinde oluşturdukları yapısal ve kimyasal değişiklikler ilk çalışmada kullanılan konlardan bağımsız 92 adet konun SEM/EDS aracılığıyla incelenmesiyle tespit edilmiştir. Veriler Pearson ki-kare testi ile %95 güven aralığında karşılaştırılmıştır. Bulgular: Solüsyonlar arasında bulanıklık varlığı değerlendirmesi sonucu elde edilen antimikrobiyal etkinlik ve SEM/EDS ile ulaşılan yapısal değişiklik açısından anlamlı bir farklılık bulunamamıştır (p > 0.05). Sonuç: Yeni bir yıkama solüsyonu olan Qmix de GPKın dezenfeksiyonunda etkin bir ajan olarak bulunmuştur. Yıkama solüsyonları tüm ölçüm zamanlarında GPKnı sterilize etmişlerdir

Keywords

• Antimikrobiyal ajanlar, enerji dağılımlı x-ışını spektroskopisi, gütaperka

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