FARKLI HIZLARDA DİSTRAKTE EDİLEN SIÇAN GASTROKNEMİUS KASINDA MEYDANA GELEN DEĞİŞİKLİKLERİN ELEKTRON MİKROSKOBU İLE İNCELENMESİ

Year 2011, Volume: 45 Issue: 2, 1 - 8, 29.11.2011

Gözde Acar , Ayfer Kaynar Ayfer Kaynar

Abstract

Fizyolojik sınırlar içinde, farklı hızlarda gerilim uygulanan sıçan gastroknemius kasında meydana gelen değişikliklerin
elektron mikroskobu ile incelenmesidir. Çalışmamızda kontrol, 0.50 mm/gün, 0.75 mm/gün olarak distrakte edilen üç adet
yetişkin Sprouge Dawley cinsi sıçan kullanılmıştır. Distraktörler, sıçanların sağ tibialarına yerleştirildikten iki gün sonra biri
0.50 mm/gün, diğeri 0.75 mm/gün olmak üzere aynı koşullar altında bir hafta boyunca distrakte edilmiş ve hayvanlar birinci
haftanın sonunda sakrifiye edilmiştir. Kontrol grubundaki sıçan ise distrakte edilmeden 5. günün sonunda sakrifiye edilmiş
alınan kas örnekleri elektron mikroskobunda incelenmiştir. Farklı hızlarda distrakte edilen kas örnekleri karşılaştırıldığında
görülen değişiklikler; glikojen yoğunluğu, mitokondri kümeleşmeleri, T tübüllerindeki dilatasyon miktarı ve
mitokondriumlarda, endoplazmik retikulumda, damar endotelinde değişiklikler şeklinde özetlenebilir. Bu pilot çalışmada elde
ettiğimiz verilerin istatistiksel değerlendirmesinin yapılabilmesi ve sonuçların kliniğe uygulanması için ileri çalışmalara
gereksinim vardır.

Keywords

Distraksiyon, gastroknemius kası, elektron mikroskobu

EVOLUATION OF THE CHANGES IN GASTROCNEMIUS MUSCLE OF RATS DISTRACTED IN DIFFERING PACES WITH ELECTRON MICROSCOPE

Abstract

Lengthening of the rodent tibia using an external device was performed to study gastrocnemius muscle subjected to stretch in
different paces. Three male Sprouge Dawley rats were used as one for control group, others for 0.50 mm, 0.75 mm
distraction per a day. The distractors were placed in right tibial bones. After the replacement of distractors one rat was set for
0.50 mm distraction through one week and the other one for 0.75 mm distraction under the same conditions. At the end of
one week, rats were sacrificed. The control rat was sacrificed without being distracted after five days of replacing the
distractor. We ended up with differing glycogen levels, mitochondrion gatherings and dilations of T tubules in the muscle
tissue specimens of distracted rats in different velocities. Further studies are needed for statistical analysis and clinical
applications.

Keywords

Distraction, gastrocnemius muscle, electron microscope

References

• Ilizarov GA. The tension-stress effect on the genesis and growth of tissues. Part I . The influence of stability of fixation and soft-tissue preservation. Clin Orthop 1989; 238: 249.
• Mc Carthy JG, Schrieber J, Karp N : Lengthening of the human mandible by gradual distraction. Plast Reconstr Surg 1993 Aug; 92 (2): 372-3.
• Bell WH, Harper Rp, Gonzales M , Cherkashin A M , Samchukov M L . Distraction oosteogenesis to widen the mandible. Br J Oral Maxillofac Surg 1997 Feb; 35 (1): 11-19.
• Block MS, Cervini D, Chang A, Gottsegen GB. Anterior maxillary advancement using tooth- supported distraction osteogenesis. J Oral Maxillofac Surg 1995; 53: 561-5.
• Sproul JT, Price CT. Recent advances in limb lengthening. Part I I biological advances. Orthopaedic Rev 1992; 21: 425-430.
• Hasse, A.R., M . Pôrksen, C.E. Zimmermann. Bilateral mandibular distraction i n adult dogs with an epiperiosteal distractor. Br J Oral Maxillofac Surg 2005. 43: 105-112.
• Amino DJ, Goguen L A , Karmody CS. Distraction osteogenesis for reconstruction of mandibular symphyseal defects. Arch Otolaryngol Head NeckSurg. 1994; 120: 911ı 916.
• Knothe Tate, M . L . , P. Niederer, U . Knothe. In vivo tracer transport through the lacunocanalicular system of rat bone in an environment devoid of mechanical loading. Bone 1998. 22: 107-117.
• Constantino PD, Friedman CD, Shindo M L : Experimental mandibular regrowth by distraction osteogenesis - long term results. Arch Otolaryngol Head Neck Surg 1993; 110: 511-18.
• El-Hakim IE, Azim A M A , El-hassan MFA, Maree SM. Preliminary investigation into the effects of electrical stimulation on mandibular distraction osteogenesis i n goats. Int J Oral Maxillofac Surg. 2004; 33: 42-47.
• Gateno J, Allen M E , Teichgraeber JF, Messesmith M L . A n in vitro study of the accuracy of a new protocol for planning distraction osteogenesis of the mandible. J Oral Maxillofac Surg 2000; 58: 985-900.
• Cohen SR, Tutrick RE, Burnstein F: Distraction osteogenesis of the human craniofacial skeleton: initial experience with a new distraction system. J Craniomaxillofac Surg 1995: 6: 368-74.
• Devkota P, Zeng BF, Tang JF, Fan CY. Histological changes of the skeletal muscle due to muscle tension: A study i n rats. Kathmandu university Medical Journal 2005; 3 (3) 281-284.
• Best T M , Loitz-Ramage B, Corr DT, Vanderby R. Hyperbaryc oxygen in the treatment of acute muscle stretch injuries, Results in animal model. A m J sports Med 1998, 26 (3): 367-372.
• Kawamura B, Hosono T, Takahashi T, Yau T, Kobayashi Y , Shibata N , Shinoda Y. Limb lengthening by means of subcutaneous osteotomy. J Bone Joint Surg 1968; 50 (A): 851.
• Ilizarov GA: The principles of the Ilizarov method. Bull Hosp Joint Dis Orthop Inst 1988; 48: 1-12.
• Hubka MJ, Taylor JAM, Schultz GD, Traina AD: Lumbar intervertebral disc herniation: chiropractic management using flexion, extension, and rotational manipulative therapy. Chiropractic Technique 1991; 3 (1): 5-12.