Genetic Characterization of Turkish Commercial Opium Poppy (Papaver somniferum L.) Cultivars Using ISSR and SSR Markers

Volume: 3 Number: 7 July 1, 2014
Gülşen Boztepe Güçlü , Tuğba Gürkök , Mesut Koyuncu , Neşet Arslan , İskender Parmaksız
EN

Genetic Characterization of Turkish Commercial Opium Poppy (Papaver somniferum L.) Cultivars Using ISSR and SSR Markers

Abstract

– Economically important plant opium poppy (Papaver somniferum L.) is a medicinal plant producing benzylisoquinoline alkaloids such as narcotic analgesic morphine and codeine, antimicrobial agent sanguinarine. In this study, it was aimed to utilize simple sequence repeat (SSR) and inter simple sequence repeat (ISSR) markers to help breeding programs of opium poppy containing high level of morphine. 15 ISSR and 5 SSR primers were studied on registered 17 cultivars in Turkey. In ISSR, total 55 bands were obtained, of which 44 were polymorphic. Average genetic distance was found to be 0.27, and Shannon index was 0.38. The least number of bands belonging to primer AT3 was 1 and the highest was 6, belonging to primers AT19, AT8 and AT15. In SSR study, 5 SSR primers were studied and 39 polymorphic bands were obtained. Average genetic distance was found to be 0.47, and Shannon index was 0.46. As a result ISSR and SSR marker systems can be used for classification of the cultivars

Keywords

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References

  1. A. Gümüşçü, N. Arslan, Comparing yield and yield components of some selected poppy (Papaver somniferum L.) lines, Turkish Journal of Agriculture and Forestry. (1998) 23: 991–997.
  2. P.J. Facchini, M. Hagel, K. Liscombe, N. Loukanina, P. MacLeod, N. Samanani, K.G., Zulak, Opium poppy: blueprint for an alkaloid factory, Phytochem Rev. (2007) 6:97–124 T.C. Toprak Mahsulleri Ofisi Genel Müdürlüğü. 2011 Yılı TMO Haşhaş raporu. http://www.tmo.gov.tr/Upload/Document/raporlar/hashassektor2011.pdf. (August 23, 2014).
  3. E. Zietkiewicz, A.D. Rafalski, Labuda Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification, Genomics. (1994). 20: 176–183.
  4. S. Hamza, W.B. Hamida, A. Rebai, M. Harrabi, SSR-based genetic diversity assessment among Tunisian winter barley and relationship with morphological traits, Euphytica. (2004). 135: 107–118.
  5. P.K. Gupta, R.K. Varshney, M. Prasad, Molecular markers: principles and methodology. In: Jain SM, Brar DS, Ahloowalia BS (eds.). Molecular techniques in crop improvement. Kluwer Academic Publishers, Dordrecht.The Netherlands. (2002) pp. 9-54.
  6. H. Budak, R.C. Shearman, I. Parmaksiz, I. Dweikat, Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs, Theoretical and Applied Genetics. (2004). 109: 280–288.
  7. M.A. Alam, P.K. Naik, G.P. Mishra. Congruence of RAPD and ISSR Markers for Evaluation of Genomic Relationship Among 28 Populations of Podophyllum hexandrum Royle from Himachal Pradesh, India, Turkish Journal of Botany. (2009). 33: 1-12.
  8. H. Bayraktar, K. Akan, Genetic characterization of Pyrenophora graminea isolates and the reactions of some barley cultivars to leaf stripe disease under greenhouse conditions, Turkish Journal of Agriculture and Forestry. (2011). 36: 329-339.
  9. H. Hamza, M.A. Benabderrahim, M. Elbekkay, G. Ferdaous, T. Triki, A. Ferchichi. Investigation of genetic variation in Tunisian date palm (Phoenix dactylifera L.) cultivars using ISSR marker systems and their relation with fruit characteristics, Turkish Journal of Biology (2012). 36: 449-458.
  10. İ. Parmaksız and S. Özcan, Morphological, chemical, and molecular analyses of Turkish Papaver accessions (Sect. Oxytona), Turkish Journal of Botany. (2011). 35: 1–16.
APA
Güçlü, G. B., Gürkök, T., Koyuncu, M., Arslan, N., & Parmaksız, İ. (2014). Genetic Characterization of Turkish Commercial Opium Poppy (Papaver somniferum L.) Cultivars Using ISSR and SSR Markers. Journal of New Results in Science, 3(7), 48-57. https://izlik.org/JA28EU84BN
AMA
1.Güçlü GB, Gürkök T, Koyuncu M, Arslan N, Parmaksız İ. Genetic Characterization of Turkish Commercial Opium Poppy (Papaver somniferum L.) Cultivars Using ISSR and SSR Markers. JNRS. 2014;3(7):48-57. https://izlik.org/JA28EU84BN
Chicago
Güçlü, Gülşen Boztepe, Tuğba Gürkök, Mesut Koyuncu, Neşet Arslan, and İskender Parmaksız. 2014. “Genetic Characterization of Turkish Commercial Opium Poppy (Papaver Somniferum L.) Cultivars Using ISSR and SSR Markers”. Journal of New Results in Science 3 (7): 48-57. https://izlik.org/JA28EU84BN.
EndNote
Güçlü GB, Gürkök T, Koyuncu M, Arslan N, Parmaksız İ (July 1, 2014) Genetic Characterization of Turkish Commercial Opium Poppy (Papaver somniferum L.) Cultivars Using ISSR and SSR Markers. Journal of New Results in Science 3 7 48–57.
IEEE
[1]G. B. Güçlü, T. Gürkök, M. Koyuncu, N. Arslan, and İ. Parmaksız, “Genetic Characterization of Turkish Commercial Opium Poppy (Papaver somniferum L.) Cultivars Using ISSR and SSR Markers”, JNRS, vol. 3, no. 7, pp. 48–57, July 2014, [Online]. Available: https://izlik.org/JA28EU84BN
ISNAD
Güçlü, Gülşen Boztepe - Gürkök, Tuğba - Koyuncu, Mesut - Arslan, Neşet - Parmaksız, İskender. “Genetic Characterization of Turkish Commercial Opium Poppy (Papaver Somniferum L.) Cultivars Using ISSR and SSR Markers”. Journal of New Results in Science 3/7 (July 1, 2014): 48-57. https://izlik.org/JA28EU84BN.
JAMA
1.Güçlü GB, Gürkök T, Koyuncu M, Arslan N, Parmaksız İ. Genetic Characterization of Turkish Commercial Opium Poppy (Papaver somniferum L.) Cultivars Using ISSR and SSR Markers. JNRS. 2014;3:48–57.
MLA
Güçlü, Gülşen Boztepe, et al. “Genetic Characterization of Turkish Commercial Opium Poppy (Papaver Somniferum L.) Cultivars Using ISSR and SSR Markers”. Journal of New Results in Science, vol. 3, no. 7, July 2014, pp. 48-57, https://izlik.org/JA28EU84BN.
Vancouver
1.Gülşen Boztepe Güçlü, Tuğba Gürkök, Mesut Koyuncu, Neşet Arslan, İskender Parmaksız. Genetic Characterization of Turkish Commercial Opium Poppy (Papaver somniferum L.) Cultivars Using ISSR and SSR Markers. JNRS [Internet]. 2014 Jul. 1;3(7):48-57. Available from: https://izlik.org/JA28EU84BN