Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries

Cetin Cekic

EN

Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries

Abstract

Bulked segregant analysis was used to determine inter-simple sequence repeat DNA (ISSR) markers for two genes, one controls seasonality and other runnering in diploid Fragaria spp. ISSR- PCR analysis was performed using these DNA pools as template. The bulks are screened for differences using anchored simple sequence polymorphic DNA primers which generates clean band profiles and polymorphic fragments were detected. Using 23 primers, either singly or pairwise, we established 16 markers tightly linked to the seasonality locus and 7 markers for runnering locus. This bulked segregant method can be applied for any species and any region of interest in which detailed linkage maps or physical maps are needed.

Keywords

Strawberry, ISSR, Bulk, Marker.

References

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Cetin Cekic

Publication Date

September 1, 2015

Submission Date

February 7, 2016

Acceptance Date

-

Published in Issue

Year 2015 Volume: 4 Number: 9

IZ

https://izlik.org/JA46WY99WX

APA

Cekic, C. (2015). Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries. Journal of New Results in Science, 4(9), 92-98. https://izlik.org/JA46WY99WX

AMA

1.Cekic C. Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries. JNRS. 2015;4(9):92-98. https://izlik.org/JA46WY99WX

Chicago

Cekic, Cetin. 2015. “Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries”. Journal of New Results in Science 4 (9): 92-98. https://izlik.org/JA46WY99WX.

EndNote

Cekic C (September 1, 2015) Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries. Journal of New Results in Science 4 9 92–98.

IEEE

[1]C. Cekic, “Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries”, JNRS, vol. 4, no. 9, pp. 92–98, Sept. 2015, [Online]. Available: https://izlik.org/JA46WY99WX

ISNAD

Cekic, Cetin. “Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries”. Journal of New Results in Science 4/9 (September 1, 2015): 92-98. https://izlik.org/JA46WY99WX.

JAMA

1.Cekic C. Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries. JNRS. 2015;4:92–98.

MLA

Cekic, Cetin. “Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries”. Journal of New Results in Science, vol. 4, no. 9, Sept. 2015, pp. 92-98, https://izlik.org/JA46WY99WX.

Vancouver

1.Cetin Cekic. Bulk Segregant Analysis Technique to Target Seasonality and Runnering Loci in Diploid Strawberries. JNRS [Internet]. 2015 Sep. 1;4(9):92-8. Available from: https://izlik.org/JA46WY99WX