The influence of olive leaf extract on the activity of antioxidant enzymes in the Hemolymph of Galleria mellonella (L.) (Lepidoptera: Pyralidae)
Abstract
The present study has aimed to examine the effects of olive leaf extract on the antioxidant enzyme activity of the invertebrate model organism Galleria mellonella L. (Lepidoptera: Pyralidae) hemolymph. For this purpose, after the olive leaves collected from Çanakkale province were dried at room temperature, the ethanolic extract was obtained with a soxhlet. After the ethanol was removed from the obtained extract in the rotary evaporator, the obtained dry matter was prepared in the phosphate buffer salt, 72 mg mL-1 as the highest dose and the lowest 0.010 mg mL-1 and injected into the larvae reared for the experiment. Hemolymph was collected at 4, 8, 12, 16, and 24-hours post-injection. Catalase (CAT) and superoxide dismutase (SOD) enzyme activities and malondialdehyde (MDA) formation levels were determined spectrophotometrically in the collected hemolymph. According to the results obtained, olive leaf extract decreased CAT and SOD activity in G. mellonella hemolymph at certain doses and increased the amount of MDA. For all enzymes, group, dose, and group-dose interactions were statistically significant. In addition, as a result of the examination made in terms of time, it was seen that the measurement of enzyme activities at the 24th hour was significant. The results of our study show that the antioxidant capacity of the olive leaf cannot be transferred to other living things and even reduces the antioxidant capacity of other organisms.
Keywords
Olea europaea, Galleria mellonella, Catalase, Superoxide dismutase, Malondialdehyde
Supporting Institution
Çanakkale Onsekiz Mart University The Scientific Research Coordination Unit,
Project Number
Project number: FHD-2020-3336
Ethical Statement
Our study was conducted and written in accordance with ethical standards.
Thanks
we are grateful to ÇOMÜDAM authorities and workers who provided us with laboratory facilities during the study.
References
- C. La Vecchia, Mediterranean diet and cancer, Public Health Nutrition 7 (7) (2004) 965–968.
- M. N. Vissers, P. L. Zock, M. B. Katan, Bioavailability and antioxidant effects of olive oil phenols in humans: a review, European Journal of Clinical Nutrition 58 (6) (2004) 955–965.
- O. Benavente-Garcia, J. Castillo, J. Lorente, A. D. R. J. Ortuño, J. A. Del Rio, Antioxidant activity of phenolics extracted from Olea europaea L. leaves, Food Chemistry 68 (4) (2000) 457–462.
- N. Zorić, I. Kosalec, The antimicrobial activities of oleuropein and hydroxytyrosol, in: M. Rai, I. Kosalec, (Eds.), Promising antimicrobials from natural products, Cham: Springer International Publishing, Switzerland AG, 2022, pp. 75–89.
- A. Bianco, N. Uccella, Biophenolic components of olives, Food Research International 33 (6) (2000) 475–485.
- M. Tasioula‐Margari, O. Okogeri, Isolation and characterization of virgin olive oil phenolic compounds by HPLC/UV and GC‐MS, Journal of Food Science 66 (4) (2001) 530–534.
- G. Bisignano, A. Tomaino, R. L. Cascio, G. Crisafi, N. Uccella, A. Saija, On the in-vitro antimicrobial activity of oleuropein and hydroxytyrosol, Journal of Pharmacy and Pharmacology 51 (8) (1999) 971–974.
- G. Samuelsson, The blood pressure lowering factor in leaves of Olea europaea, Farmacevtisk Revy 15 (1) (1951) 229–239.
- A. Zarzuelo, J. Duarte, J. Jimenez, M. Gonzalez, M. P. Utrilla, Vasodilator effect of olive leaf, Planta Medica 57 (05) (1991) 417–419.
- K. Qabaha, F. Al-Rimawi, A. Qasem, S. A. Naser, S.A., Oleuropein is responsible for the major anti-inflammatory effects of olive leaf extract, Journal of Medicinal Food 21 (3) (2018) 302–305.