Green Synthesis of Zinc Oxide Nanoparticles from Malus domestica, Tilia cordata, and its Biological Activitiess
Abstract
This study aimed to evaluate the anticancer and antibacterial drug status of biomass, including zinc oxide (ZnO) nanoparticles (NPs) synthesized from two plant extracts according to the results of Topoisomerase-I enzyme inhibition, DNA cleavage, DNA binding, and antibacterial activity tests. The Malus domestica (apple) and Tilia cordata (linden) plants were dried and powdered, and their extracts were prepared in pure water. X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared (FTIR) analyses were applied for the characterization of the light-yellow powder ZnO NPs. Additionally, Topoisomerase-I enzyme inhibition, DNA cleavage, and DNA binding analyses were performed by agarose gel electrophoresis method. Powder ZnO NPs (biomass) were analyzed by the agar well diffusion method by using two gram-positive and two gram-negative bacteria. According to the XRD analysis results, the peaks obtained show that there is no foreign matter or secondary diffraction peak other than the hexagonal ZnO structure. It was observed that ZnO NPs from Tilia cordata extract are effective against Klebsiella pneumoniae. ZnO NPs from Malus domestica extract have the potential to be an effective human Topoisomerase-I enzyme inhibitor. There was poor DNA cleavage activity in all samples. In ZnO NPs from linden extract, there was an interaction at a 1mg/mL concentration of CT-DNA.
Keywords
Enzyme inhibition, green synthesis, inorganic drug, anti-bacterial activity, ZnO NPs
References
- H. Agarwal, S. Venkat Kumar, S. Rajeshkumar, A review on green synthesis of zinc oxide nanoparticles – An eco-friendly approach, Resource-Efficiency and Technologies 3 (4) (2017) 406–413.
- R. Prasad, A. Bhattacharyya, Q. D. Nguyen, Nanotechnology in sustainable agriculture: Recent developments, challenges, and perspectives, Frontiers in Microbiology 8 (2017) 1014.
- C. O. Dimkpa, P. S. Bindraban, Fortification of micronutrients for efficient agronomic production: a review, Journal of Agricultural and Food Chemistry 36 (2016) 7.
- G. Marslin, K. Siram, Q. Maqbool, R. K. Selvakesavan, D. Kruszka, P. Kachlicki, G. Franklin, Secondary metabolites in the green synthesis of metallic nanoparticles, Materials 11 (6) (2018) 940.
- S. Gunalan, R. Sivaraj, V. Rajendran, Green synthesized ZnO nanoparticles against bacterial and fungal pathogens, Progress in Natural Science: Materials International 22 (6) (2012) 693–700.
- N. Bala, S. Saha, M. Chakraborty, M. Maiti, S. Das, R. Basu, P. Nandy, Green synthesis of zinc oxide nanoparticles using Hibiscus subdariffa leaf extract: effect of temperature on synthesis, anti-bacterial activity and anti-diabetic activity, Royal Society of Chemistry Advances 5 (7) (2015) 4993-5003.
- A. A. Zahir, A. A. Rahuman, Evaluation of different extracts and synthesised silver nanoparticles from leaves of Euphorbia prostrata against Haemaphysalis bispinosa and Hippobosca maculata, Veterinary Parasitology 187 (3-4) (2012) 511–520.
- K. Gudikandula, S. Charya Maringanti, Synthesis of silver nanoparticles by chemical and biological methods and their antimicrobial properties, Journal of Experimental Nanoscience 11 (9) (2016) 714–721.
- S. Kunjiappan, R. Chowdhury, C. Bhattacharjee, A green chemistry approach for the synthesis and characterization of bioactive gold nanoparticles using Azolla microphylla methanol extract, Frontiers of Materials Science 8 (2014) 123–135.
- M. Gomathi, P. V. Rajkumar, A. Prakasam, K. Ravichandran, Green synthesis of silver nanoparticles using Datura stramonium leaf extract and assessment of their antibacterial activity, Resource Efficiency and Technologies 3 (2017) 280–284.