Testing the usage of ITS2 and 28S-D2 markers in identifying some species of Ammophila, Prionyx, and Sphex genera (Hymenoptera: Sphecidae)

Yıl 2015, Cilt: 4 Sayı: 9, 11 - 17, 29.05.2015

Yasar Gulmez Ilyas Can

Öz

In addition to traditional methods, investigation of rDNA gene regions with molecular techniques is widely used to identify insect taxa. In this study, the usability of the primers (TrITS2, 28S-D2) belonging to second expansion segment (D2) of the 28S subunit (28S-D2) and second internal transcribed spacer (ITS2) rDNA gene regions were tested to identify and differentiate six species of Sphecidae family namely Ammophila sabulosa, Ammophila heydeni, Prionyx kirbyi, Prionyx nudatus, Sphex flavipennis, Sphex funerarius for the first time. DNA isolated from samples were amplified by Polymerase Chain Reaction (PCR) and digested by restriction enzymes. PCR products and restriction enzyme analysis of ITS2 obtained from Prionyx species displayed species-specific DNA bands indicating that these species could be identified by using TrITS2 primers and restriction enzymes. However, neither DNA bands nor the sequence analysis of 28S-D2rDNA primers provided species-specific data. Consequently, ITS2 marker was suitable for only one of the genera (Prionyx), whereas 28S-D2 marker was suitable for none of the taxa examined

Anahtar Kelimeler

Testing, ITS2, identifying

Kaynakça

• of California Press, Berkeley, Los Angeles, London. pp. IX+695, 1976.
• (Hymenoptera: Apoidea: Sphecidae: Sphecini). J Hym Res 9 (2000), 324-346.
• (Hymenoptera: Apoidea) basierend auf molekularen Daten. M D Gesell Allg Ange 16 (2008), 99–
• (Hymenoptera: Braconidae): Combined Analysis of 16S and 28S rDNA Genes and Morphological Data. Mol Phylogenet Evol 10 (1998), 354-366.
• Evolution of Sociality in Wasps. Mol Phylogenet Evol 9 (1998), 183-191.
• Encarsia luteola (Hymenoptera: Aphelinidae). Ann Entomol Soc Am 93 (2000), 738-744.
• (Hymenoptera: Trichogrammatidae) species in China. Eur J Entomol 104 (2007), 363-367.
• ITS2 Ribosomal DNA Sequence Variation of the Bumblebee, Bombus ardens (Hymenoptera: Apidae). Genes and Genomics 31(2009), 293-303. based on some morphological scales and 28S D2 rDNA with description of a new species. Turk J Zool 34 (2010), 279-289.
• Ribosomal DNA ITS2 Region in Two Trichogramma Species (Hymenoptera: Trichogrammatidae). Arch Biol Sci Belgrade, 63 (2011), 949-954. of Trichogramma turkestenica (Hymenoptera: Trichogrammatidae) which Inhabits Taif
• Governorate at the West of Saudi Arabia, Afr J Biotechnol 10 (2011), 9467-9472.
• F. H. Collins, S. Paskewitz, A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species. Insect Mol Biol 5 (1996), 1-9. including the Culex pipines complex, inferred from the internal transcribed spacer of ribosomal
• DNA. Insect Mol Biol 5 (1996), 93-107.
• ‘chagosensis’ (Porifera: Calcarea) from ribosomal DNA sequences: implications for populations history and conservation of the Great Barrier Reef World Heritage Area (Australia). Mol Ecol 11(2002), 1753-1768.
• Trichogramma (Hymenoptera: Trichogrammatidae) Using Sequences of the ITS2 Region and Restriction Analysis. Neotrop Entomol 30 (2001): 259-262.
• Babcock, Molecular systematics of the Chalcidoidea using 28S-D2 rDNA. In: Hymenoptera, evolution, biodiversity and biological control (ed. Austin AD and Dowton M), CSIRO Publishing, Melbourne, pp. 59-73 (2000).
• Hymenoptera): 28S D2 rDNA considerations of the Brachycyrtinae, Labeninae, Paxylommatinae and Xoridinae. Zool Scr 28 (1999), 203-210.
• Hymenoptera: Apocrita: evolutionary transitions among parasitic wasps. Biol J Linn Soc 74 (2001), 87