Mesenchymal stem
cells (MSCs) are a promising cell source for cell-based therapies because of
their self-renewal and multi-lineage differentiation potential. Unlike
embryonic stem cells adult stem cells are subject of aging processes and the
concomitant decline in their function. Age-related changes in MSCs have to be
well understood in order to develop clinical techniques and therapeutics based
on these cells. In this work we have
studied the effect of aging on adhesive behaviour of bone marrow-derived MSC
and MG- 63 osteoblastic cells onto three extracellular matrix proteins:
fibronectin (FN), vitronectin (VN) and collagen
I (Coll I). The results revealed substantial differences in adhesive
behaviour of both cell types during 21 days in culture. Bone-marrow derived
MSCs decreased significantly their adhesive affinity to all studied proteins
after 7th day in culture with further incubation. In contrast, MG-63 cells,
demonstrated a stable cell adhesive phenotype with high affinity to FN and Coll
I and low affinity to vitronectin over the whole culture period. These data
suggest that adhesive behaviour of MSCs to matrix proteins is affected by aging
processes unlike MG-63 cells and the age-related changes have to be considered
when expanding adult stem cells for clinical applications.
Journal Section | Articles |
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Authors | |
Publication Date | March 2, 2017 |
Published in Issue | Year 2017 Volume: 6 Issue: 1 |
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