The influence of olive leaf extract on the activity of antioxidant enzymes in the Hemolymph of Galleria mellonella (L.) (Lepidoptera: Pyralidae)

Yıl 2023, Cilt: 12 Sayı: 3, 188 - 200, 31.12.2023

Serhat Kaya Seranay Türkdoğan Tuğba Söküt Açar

https://doi.org/10.54187/jnrs.1397882

Öz

The present study has aimed to examine the effects of olive leaf extract on the antioxidant enzyme activity of the invertebrate model organism Galleria mellonella L. (Lepidoptera: Pyralidae) hemolymph. For this purpose, after the olive leaves collected from Çanakkale province were dried at room temperature, the ethanolic extract was obtained with a soxhlet. After the ethanol was removed from the obtained extract in the rotary evaporator, the obtained dry matter was prepared in the phosphate buffer salt, 72 mg mL-1 as the highest dose and the lowest 0.010 mg mL-1 and injected into the larvae reared for the experiment. Hemolymph was collected at 4, 8, 12, 16, and 24-hours post-injection. Catalase (CAT) and superoxide dismutase (SOD) enzyme activities and malondialdehyde (MDA) formation levels were determined spectrophotometrically in the collected hemolymph. According to the results obtained, olive leaf extract decreased CAT and SOD activity in G. mellonella hemolymph at certain doses and increased the amount of MDA. For all enzymes, group, dose, and group-dose interactions were statistically significant. In addition, as a result of the examination made in terms of time, it was seen that the measurement of enzyme activities at the 24th hour was significant. The results of our study show that the antioxidant capacity of the olive leaf cannot be transferred to other living things and even reduces the antioxidant capacity of other organisms.

Anahtar Kelimeler

Olea europaea, Galleria mellonella, Catalase, Superoxide dismutase, Malondialdehyde

Etik Beyan

Our study was conducted and written in accordance with ethical standards.

Destekleyen Kurum

Çanakkale Onsekiz Mart University The Scientific Research Coordination Unit,

Proje Numarası

Project number: FHD-2020-3336

Teşekkür

we are grateful to ÇOMÜDAM authorities and workers who provided us with laboratory facilities during the study.

Kaynakça

• C. La Vecchia, Mediterranean diet and cancer, Public Health Nutrition 7 (7) (2004) 965–968.
• M. N. Vissers, P. L. Zock, M. B. Katan, Bioavailability and antioxidant effects of olive oil phenols in humans: a review, European Journal of Clinical Nutrition 58 (6) (2004) 955–965.
• O. Benavente-Garcia, J. Castillo, J. Lorente, A. D. R. J. Ortuño, J. A. Del Rio, Antioxidant activity of phenolics extracted from Olea europaea L. leaves, Food Chemistry 68 (4) (2000) 457–462.
• N. Zorić, I. Kosalec, The antimicrobial activities of oleuropein and hydroxytyrosol, in: M. Rai, I. Kosalec, (Eds.), Promising antimicrobials from natural products, Cham: Springer International Publishing, Switzerland AG, 2022, pp. 75–89.
• A. Bianco, N. Uccella, Biophenolic components of olives, Food Research International 33 (6) (2000) 475–485.
• M. Tasioula‐Margari, O. Okogeri, Isolation and characterization of virgin olive oil phenolic compounds by HPLC/UV and GC‐MS, Journal of Food Science 66 (4) (2001) 530–534.
• G. Bisignano, A. Tomaino, R. L. Cascio, G. Crisafi, N. Uccella, A. Saija, On the in-vitro antimicrobial activity of oleuropein and hydroxytyrosol, Journal of Pharmacy and Pharmacology 51 (8) (1999) 971–974.
• G. Samuelsson, The blood pressure lowering factor in leaves of Olea europaea, Farmacevtisk Revy 15 (1) (1951) 229–239.
• A. Zarzuelo, J. Duarte, J. Jimenez, M. Gonzalez, M. P. Utrilla, Vasodilator effect of olive leaf, Planta Medica 57 (05) (1991) 417–419.
• K. Qabaha, F. Al-Rimawi, A. Qasem, S. A. Naser, S.A., Oleuropein is responsible for the major anti-inflammatory effects of olive leaf extract, Journal of Medicinal Food 21 (3) (2018) 302–305.
• R. Japón-Luján, J. M. Luque-Rodríguez, M. L. De Castro, Dynamic ultrasound-assisted extraction of oleuropein and related biophenols from olive leaves, Journal of Chromatography A 1108 (1) (2006) 76–82.
• M. Y. Bektay, E. M. Güler, M. Gökçe, M. V. Kızıltaş, Investigation of the genotoxic, cytotoxic, apoptotic, and oxidant effects of olive leaf extracts on liver cancer cell lines, Turkish Journal of Pharmaceutical Sciences 18 (6) (2021) 781–789.
• A. Saija, D. Trombetta, A. Tomaino, R. L. Cascio, P. Princi, N. Uccella, F. Castelli, In vitro'evaluation of the antioxidant activity and biomembrane interaction of the plant phenols oleuropein and hydroxytyrosol, International Journal of Pharmaceutics 166 (2) (1998) 123–133.
• F. Visioli, A. Poli, C. Gall, Antioxidant and other biological activities of phenols from olives and olive oil, Medicinal Research Reviews 22 (1) (2002) 65–75.
• I. Andreadou, E. Mikros, K. Ioannidis, F. Sigala, K. Naka, S. Kostidis, E. K. Iliodromitis, Oleuropein prevents doxorubicin-induced cardiomyopathy interfering with signaling molecules and cardiomyocyte metabolism, Journal of Molecular and Cellular Cardiology 69 (2014) 4–16.
• P. Bogani, C. Galli, M. Villa, F. Visioli, Postprandial anti-inflammatory and antioxidant effects of extra virgin olive oil, Atherosclerosis 190 (1) (2007) 181–186.
• J. Casas-Sanchez, M. A. Alsina, M. K. Herrlein, C. Mestres, Interaction between the antibacterial compound, oleuropein, and model membranes, Colloid and Polymer Science 285 (2007) 1351–1360.
• Y. Liu, L. C. McKeever, N. S. Malik, Assessment of the antimicrobial activity of olive leaf extract against foodborne bacterial pathogens, Frontiers in Microbiology 8 (2017) Article Number 113 8 pages.
• A. Karković Marković, J. Torić, M. Barbarić, C. Jakobušić Brala, Hydroxytyrosol, tyrosol and derivatives and their potential effects on human health, Molecules 24 (10) (2019) 2001.
• M. D. Lavine, M. R. Strand, Insect hemocytes and their role in immunity, Insect Biochemistry and Molecular Biology 32 (10) (2002) 1295–1309.
• G. Wu, Y. Yi, Effects of dietary heavy metals on the immune and antioxidant systems of Galleria mellonella larvae, Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 167 (2015) 131–139.
• G. W. Felton, C. B. Summers, Antioxidant systems in insects, Archives of Insect Biochemistry and Physiology 29 (2) (1995) 187–197.
• Y. Wang, L. W. Oberley, D. W. Murhammer, Antioxidant defense systems of two lipidopteran insect cell lines, Free Radical Biology and Medicine 30 (11) (2001) 1254–1262.
• J. C. Lee, Y. O. Son, P. Pratheeshkumar, X. Shi, Oxidative stress and metal carcinogenesis, Free Radical Biology and Medicine 53 (4) (2012) 742–757.
• T. P. Dalton, H. G. Shertzer, A. Puga, Regulation of gene expression by reactive oxygen, Annual Review of Pharmacology and Toxicology 39 (1) (1999) 67–101.
• E. M. Ha, C. T. Oh, Y. S. Bae, W. J. Lee, A direct role for dual oxidase in Drosophila gut immunity, Science 310 (5749) (2005) 847–850.
• K. Kavanagh, J. P. Fallon, Galleria mellonella larvae as models for studying fungal virulence, Fungal Biology Reviews 24 (1-2) (2010) 79–83.
• G. Ménard, A. Rouillon, V. Cattoir, P. Y. Donnio, Galleria mellonella as a suitable model of bacterial infection: past, present and future, Frontiers in Cellular and Infection Microbiology 11 (2021) Article Number 782733 18 pages.
• M. Asai, Y. Li, S. M. Newton, B. D. Robertson, P. R. Langford, P.R. Galleria mellonella intracellular bacteria pathogen infection models: the ins and outs, FEMS Microbiology Reviews 47 (2) (2023) 1– 32.
• T. C. Pereira, P. P. De Barros, L. R. D. O. Fugisaki, R. D. Rossoni, F. D. C. Ribeiro, R. T. De Menezes, L. Scorzoni, Recent advances in the use of Galleria mellonella model to study immune responses against human pathogens, Journal of Fungi 4 (4) (2018) Article Number 128 19 Pages.
• R. D. Rossoni, F. de Camargo Ribeiro, H. F. S. Dos Santos, J. D. Dos Santos, N. de Sousa Oliveira, M. T. Dos Santos Dutra, J. C. Junqueira, Galleria mellonella as an experimental model to study human oral pathogens, Archives of Oral Biology 101 (2019) 13–22.
• I. M. Dubovskiy, V. V. Martemyanov, Y. L. Vorontsova, M. J. Rantala, E. V. Gryzanova, V. V. Glupov, Effect of bacterial infection on antioxidant activity and lipid peroxidation in the midgut of Galleria mellonella L. larvae (Lepidoptera, Pyralidae), Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 148 (1) (2008) 1–5.
• I. M. Dubovskii, E. V. Grizanova, E. A. Chertkova, I. A. Slepneva, D. A. Komarov, Y. L. Vorontsova, V. V. Glupov, Generation of reactive oxygen species and activity of antioxidants in hemolymph of the moth larvae Galleria mellonella (L.) (Lepidoptera: Piralidae) at development of the process of encapsulation, Journal of Evolutionary Biochemistry and Physiology 46 (2010) 35–43.
• H. . Altuntaş, Determination of gibberellic acid (GA3)-induced oxidative stress in a model organism Galleria mellonella L. (Lepidoptera: Pyralidae), Environmental Entomology 44 (1) (2015) 100–105.
• H. Altuntaş, E. Duman, G. Kılıç, Juglone induced oxidative and genotoxic stress in the model insect Galleria mellonella L. (Pyralidae: Lepidoptera), International Journal of Tropical Insect Science 40 (2020) 611–619.
• B. Dere, H. Altuntaş, Z. U. Nurullahoğlu, Insecticidal and oxidative effects of azadirachtin on the model organism Galleria mellonella L. (Lepidoptera: Pyralidae), Archives of Insect Biochemistry and Physiology 89 (3) (2015) 138–152.
• S. Kaya, Assessment of the effect of thymbra capitata ethanolic extract on galleria mellonella hemolymph antioxidant enzymes, Commagene Journal of Biology 6 (2) (2022) 178–183.
• S. Kaya, N. Demir, Effects of olive (olea europaea) leaf extract on hemocyte-mediated immune responses of the model organism galleria mellonella. Turkish Journal of Agricultural and Natural Sciences 7 (3) (2020) 646–653.
• S. Kaya, S. Türkdoğan, The olive leaf extract induced phenoloxidase activity changes in galleria mellonella hemolymph, Turkish Journal of Agricultural and Natural Sciences 8 (4) (2021) 934-939.
• J. Bronskill, A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pyralidae). Journal of the Lepidopterists' Society 15 (2) (1961) 102–104.
• M. M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analytical Biochemistry 72 (1-2) (1976) 248–254.
• L. Flöhe F. Ötting, Superoxide dismutase assays, Methods of Enzymology 105 (1984) 93–104.
• H. Aebi, Catalase in vitro, Methods in Enzymology 105 (1984) 121–126.
• J. A. Buege, S. D. Aust, Microsomal lipid peroxidation, In Methods in Enzymology 52 (1978) 302–310.
• S. Kaya, T. S. Açar, Zenodo (2023), https://zenodo.org/records/8167215, Accessed 20 July 2023.
• P. M. Furneri, A. Marino, A. Saija, N. Uccella, G. Bisignano, In vitro antimycoplasmal activity of oleuropein, International Journal of Antimicrobial Agents 20 (4) (2002) 293–296.
• R. Pennisi, I. Ben Amor, B. Gargouri, H. Attia, R. Zaabi, A. B. Chira, M. T. Sciortino, Analysis of antioxidant and antiviral effects of olive (olea europaea l.) leaf extracts and pure compound using cancer cell model, Biomolecules 13 (2) (2023) Article Number 238 17 Pages.
• A. Gholamhosseini, M. R. Kheirandish, N. Shiry, M. Akhlaghi, S. Soltanian, H. Roshanpour, M. Banaee, Use of a methanolic olive leaf extract (Olea europaea) against white spot virus syndrome in Penaeus vannamei: Comparing the biochemical, hematological and immunological changes, Aquaculture 528 (2020) Article Number 735556 10 pages.
• H. F. M. Al-Azzawie, S. S. Alhamdani, Hypoglycemic and antioxidant effect of oleuropein in alloxan-diabetic rabbits, Life Sciences 78 (12) (2006) 1371–1377.
• H. Jemai, A. El Feki, S. Sayadi, Antidiabetic and antioxidant effects of hydroxytyrosol and oleuropein from olive leaves in alloxan-diabetic rats, Journal of Agricultural and Food Chemistry 57 (19) (2009) 8798–8804.
• I. Fki, M. Bouaziz, Z. Sahnoun, S. Sayadi, Hypocholesterolemic effects of phenolic-rich extracts of Chemlali olive cultivar in rats fed a cholesterol-rich diet, Bioorganic & Medicinal Chemistry 13(18) (2005) 5362–5370.
• S. Lockyer, P. Yaqoob, J. P. Spencer, I. Rowland, Olive leaf phenolics and cardiovascular risk reduction: physiological effects and mechanisms of action, Nutrition and Aging 1(2) (2012) 125–140.
• H. Rajabiesterabadi, A. Ghelichi, S. Jorjani, S. M. Hoseini, R. Akrami, Dietary olive (Olea europaea) leaf extract suppresses oxidative stress and modulates intestinal expression of antioxidant-and tight junction-related genes in common carp (Cyprinus carpio), Aquaculture 520 (2020) Article Number 734676 8 pages.
• Y. Wang, S. Wang, W. Cui, J. He, Z. Wang, X. Yang, Olive leaf extract inhibits lead poisoning-induced brain injury, Neural Regeneration Research 8 (22) (2013) 2021–2029.
• S. C. Edgecombe, G. L. Stretch, P. J. Hayball, Oleuropein, an antioxidant polyphenol from olive oil, is poorly absorbed from isolated perfused rat intestine, The Journal of Nutrition 130 (12) (2000) 2996–3002.
• S. C. Edgecombe, G. L. Stretch, P. J. Hayball, Oleuropein, an antioxidant polyphenol from olive oil, is poorly absorbed from isolated perfused rat intestine, The Journal of Nutrition 130 (12) (2000) 2996–3002.