Evaluation of the association of anti-thyroid peroxidase with antinuclear antibodies and different antinuclear antibodies patterns

Yıl 2023, , 311 - 314, 30.08.2023

Alper Togay , Banu Isbilen Basok , Ayfer Çolak , Nisel Yılmaz

https://doi.org/10.47582/jompac.1317134

Öz

Aims: To investigate the relationship between anti-thyroid peroxidase (TPO), a marker for Hashimoto’s thyroiditis, and antinuclear antibodies (ANA) and ANA patterns, biomarkers for systemic autoimmune diseases.
Methods: In this study, ANA and anti-TPO test results, obtained in our hospital laboratory between 2019 and 2022, were retrospectively evaluated. ANA was detected by the indirect immunofluorescence antibody method using commercial HEp-20-10 cell substrates and anti-TPO was determined by the sandwich immunoassay method using a commercial immunoassay analyzer.
Results: A total of 1750 patients’ results were analyzed. ANA was positive in 28.7% of anti-TPO-positive patients and 19% of anti-TPO-negative patients. While 6.4% (112/1750) of patients were positive for both ANA and anti-TPO, both test results were negative in 62.85% of patients (p<0.001). When the ANA patterns’ distribution in patients with ANA and anti-TPO positivity examined together, the homogeneous pattern was statistically significantly higher than the other patterns (p=0.043).
Conclusion: Individuals with autoimmune thyroid disease had a higher rate of autoantibodies not only to thyroid-specific antigens but also to non-thyroid-specific antigens. Further studies are needed on how epigenetic changes, such as histone modifications might cause other autoimmune diseases and increase their frequency.

Anahtar Kelimeler

Autoimmunity, antinuclear antibodies, autoimmune disease, thyroid diseases, Hashimoto disease

Anti-tiroid peroksidazın antinükleer antikorlar ve farklı antinükleer antikor paternleri ile ilişkisinin değerlendirilmesi

Öz

Arka plan: Otoimmün hastalıkların birlikteliği iyi bilinmektedir, ancak bunlara neden olan mekanizmalar henüz tam olarak anlaşılamamıştır. Histon modifikasyonları gibi çekirdekteki değişikliklerin otoimmün tiroid hastalıklarının nedeni olabileceğine dair artan kanıtlar vardır. Histon ve diğer nükleer antijenlere karşı oluşan antikorların sistemik otoimmün hastalıklara neden olabildiği bilinmektedir.
Amacımız, Hashimoto tiroiditi için bir belirteç olan anti-tiroid peroksidaz (TPO) ile sistemik otoimmün hastalıkların biyobelirteçleri olan antinükleer antikorlar (ANA) ve ANA paternleri arasındaki ilişkiyi araştırmaktır.
Yöntemler: Bu çalışmada 2019-2022 yılları arasında hastanemiz laboratuvarında elde edilen ANA ve anti-TPO test sonuçları retrospektif olarak değerlendirildi. ANA, ticari HEp-20-10 hücre substratları kullanılarak dolaylı immünofloresan antikor yöntemiyle tespit edildi ve anti-TPO, ticari bir immünoassay analiz cihazı kullanılarak sandviç immünoassay yöntemiyle belirlendi.
Bulgular: Toplam 1750 hastanın sonuçları analiz edildi. ANA, anti-TPO pozitif hastaların %28,7'sinde ve anti-TPO negatif hastaların %19'unda pozitifti. Hastaların %6,4'ü (112/1750) hem ANA hem de anti-TPO için pozitif bulunurken, hastaların %62,85'inde her iki test negatifti (p <0,001). ANA ve anti-TPO pozitifliği olan hastalarda ANA paternlerinin dağılımı birlikte incelendiğinde, homojen patern diğer paternlere göre istatistiksel olarak anlamlı derecede yüksekti (p=0,043).
Sonuçlar: Otoimmün tiroid hastalığı olan bireylerde, yalnızca tiroide özgü antijenlere karşı değil, aynı zamanda tiroide özgü olmayan antijenlere karşı da daha yüksek oranda otoantikorlar vardır. Histon modifikasyonları gibi değişikliklerin diğer otoimmün hastalıklara nasıl yol açabileceği ve sıklığını artırabileceği konusunda daha fazla çalışmaya ihtiyaç vardır.

Anahtar Kelimeler

ANA, Anti-TPO, otoimmün hastalık, otoimmün tiroid hastalığı

Kaynakça

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