Genişlemiş spektrumlu beta-laktamaz üreten ve üretmeyen Klebsiella spp. ve Escherichia coli suşlarında beta-laktam dışı antibiyotiklerin in vitro etkinliğinin değerlendirilmesi

Abstract

Amaç: Bakteriler birçok antibiyotiklere farklı mekanizmaları kullanarak direnç geliştirmektedir. Genişlemiş spektrumlu beta-laktamaz (GSBL)-üreten bakterilerin pek çok antibiyotiğe karşı dirençli olmaları tedavi seçeneklerimizi kısıtlamaktadır. Bu çalışmada in vitro olarakEscherichia coli (E. coli) ve Klebsiellaspp.suşlarında beta-laktamdışıantibiyotiklerinetkinliğiniaraştırdık. GereçveYöntem:Çalışmamızdakankültüründenizoleedilennozokomiyalkökenli97 GSBL-negatif (61E. coli, 36Klebsiellaspp.) ve 54 GSBL-pozitif (33E.coli, 21Klebsiellaspp.) suşunda GSBL varlığıfenotipikdoğrulamatestiilearaştırıldı. Aminoglikozidvekinolongrubuantibiyotiklerin in vitro etkinlikleri agar dilüsyonyöntemiilebelirlendi. Bulgular:GSBL-üretenveüretmeyensuşlarınduyarlılıkoranlarısırasıylasiprofloksasiniçin %81,4-48,1;levofloksasiniçin %85,5-50;ofloksasinvemoksifloksasiniçin %81,4-46,3;gentamisiniçin %99-37;netilmisiniçin %97,9-57,4 veamikasiniçinde %99-96,2 olarakbulundu. Sonuç: Çalışılmamızda E. coliveKlebsiellasppsuşlarındatümaminoglikozidvekinolongrubuantibiyotiklerinetkinliğinindüşükolduğu;amikasininenyüksek in vitro etkinliğe sahip olduğu görüldü

Keywords

• Genişlemiş spektrumlu beta-laktamaz
• aminoglikozid
• kinolon
• minimum inhibitor konsantrasyon

Evaluation of the in vitro efficacy of non-beta-lactam antibiotics in extended-spectrum beta-lactamase-producing and non-producing Klebsiella spp. and Escherichia coli strains

Abstract

Objective: Bacteria develop resistance to many antibiotics by using different mechanisms. The resistance of bacteria secreting extended-spectrum beta-lactamases to many antibiotics limits our treatment options. In this study, we investigated the in vitro efficacies of non-beta-lactam antibiotics in Escherichia coli (E. coli) and Klebsiella spp. strains. Material and Method: In our study, we investigated the presence of ESBL in 97 ESBL-negative (61 E. coli, 36 Klebsiella spp.) and 54 ESBL-positive (33 E. coli, 21 Klebsiella spp.) strains of nosocomial origin isolated from blood culture through a phenotypic confirmation test. We determined in vitro efficacies of aminoglycoside and quinolone group antibiotics by the agar dilution method. Results: The susceptibility rates of ESBL-producing and non-producing strains were 81.4% -48.1% to ciprofloxacin, 85.5%-50% to levofloxacin, 81.4%-46.3% to ofloxacin and moxifloxacin, 99%-37% to gentamicin, 97.9%-57.4% to netilmicin, and 99%-96.2% to amikacin, respectively. Conclusion: In our study, we found that all aminoglycoside and quinolone group antibiotics showed low efficacies.Amikacin had the highest in vitro activity in E. coli and Klebsiellaspp. strains.

Keywords

• Extended-spectrum beta-lactamase
• aminoglycoside
• quinolone
• minimal inhibitory concentration

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