journal of mtu

Journal of Medical Topics and Updates

2822-4094

Malatya Turgut Özal Üniversitesi

10.58651/jomtu.1895674

Cancer Cell Biology

Kanser Hücre Biyolojisi

Doksorubisin ile Tedavi Edilen MCF-7 Meme Kanseri Hücrelerinde Melatonin ve Fukoidanın Oksidatif Stres ve Apoptoz Üzerindeki Farklılaştırıcı Etkileri

Differential Modulation of Oxidative Stress and Apoptosis by Melatonin and Fucoidan in Doxorubicin-Treated MCF-7 Breast Cancer Cells

https://orcid.org/0000-0003-2923-5869

Korkmaz

Oya

MALATYA TURGUT ÖZAL ÜNİVERSİTESİ, TIP FAKÜLTESİ

05 01 2026

5 1 1 9 02 23 2026 04 29 2026

2022

Journal of Medical Topics and Updates

Amaç: Doksorubisin (DOX), östrojen reseptörü (ER)-pozitif meme kanserinin tedavisinde kullanılan temel kemoterapötik ajanlardan biridir. Sitotoksik etkileri büyük ölçüde mitokondriyal reaktif oksijen türleri (ROS) üretimi ve mitokondriye bağımlı apoptozun aktivasyonu aracılığıyla gerçekleşir. Bununla birlikte, seçici redoks modülatörlerinin DOX ile tetiklenen mitokondriyal stres yanıtlarını nasıl etkilediği ve bu etkilerin ER-bağımlı sinyal yolaklarıyla kesişip kesişmediği tam olarak açıklığa kavuşturulmamıştır. Bu çalışmada, melatonin ve fukoidanın DOX kaynaklı oksidatif stres ve apoptoz üzerindeki etkilerinin ER-pozitif MCF-7 meme kanseri hücrelerinde karşılaştırılması amaçlanmıştır.Materyal ve Metot: MCF-7 hücreleri 24 saat boyunca DOX (1 µM), melatonin (1 mM), fukoidan (100 µg/mL) veya bunların kombinasyonlarına maruz bırakıldı. Hücre canlılığı MTT yöntemi ile değerlendirildi. ROS düzeyleri DCFH-DA floresansı ile ölçülerek kontrol grubuna göre normalize edildi. Bax, Bcl-2, aktif (cleaved) kaspaz-3 ve ERα protein ekspresyonları ELISA yöntemi ile kantitatif olarak analiz edildi.Bulgular: DOX uygulaması hücre canlılığını anlamlı düzeyde azaltırken ve ROS üretimini artırırken (p

Objective: Doxorubicin (DOX) is a primary chemotherapeutic agent for estrogen receptor (ER)-positive breast cancer. Its cytotoxic effects are largely mediated by mitochondrial reactive oxygen species (ROS) production and the activation of mitochondria-dependent apoptosis. However, it remains unclear how selective redox modulators influence DOX-triggered mitochondrial stress responses and whether these effects intersect with ER-dependent signaling. This study aimed to compare the impacts of melatonin and fucoidan on DOX-induced oxidative stress and apoptosis in ER-positive MCF-7 breast cancer cells.Materials and Methods: MCF-7 cells were exposed for 24 hours to DOX (1 µM), melatonin (1 mM), fucoidan (100 µg/mL), or their combinations. Cell viability was assessed using the MTT assay. ROS levels were measured via DCFH-DA fluorescence and normalized to controls. Protein expression of Bax, Bcl-2, cleaved caspase-3, and ERα was quantified by ELISA.Results: DOX significantly decreased cell viability and elevated ROS production (p

Doxorubicin Melatonin Fucoidan Oxidative Stress Estrogen Receptor Alpha

Doksorubisin Melatonin Fukoidan Oksidatif Stres Östrojen Reseptör Alfa

None.

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