Health Risk Assessment of Some Heavy Metals in Lipsticks Sold in Local Markets in Iraq

Abstract

Heavy metals found in cosmetics are a safety threat to the health of consumers. Therefore, in this study, we evaluated the levels of heavy metals such as cadmium (Cd), chromium (Cr), copper (Cu), lead (Pb), zinc (Zn), and arsenic (As) in lipstick makeup. The level of heavy metals in lipstick was discovered by using an analytical technique with high selectivity and sensitivity, namely atomic absorption spectrometry. Twenty lipsticks were selected from the same brands, yet differing in price. Ten original (expensive) and ten knockoff (cheap) lipsticks were chosen from shops in Diyala, Iraq. The detection-limit (LOD) was in between 0.01 and 0.1, the quantification-limit (LOQ) was within 0.03 and 0.33, the recovery values (Rec.%) ranged from 100.17% to 101.1%, the RE values were 0.81%, and the RSD values were 1.33%. The results also revealed that the levels of metals are in the order of Pb > Cd > Zn > Cu > As > Cr. However, the levels of heavy metals that were estimated in this study were less than the permissible limit set by the executive authorities, so there seems to be no concern associated with these heavy metals. However, the daily and frequent use of lipstick by women exposes them to low levels of toxic metals as these metals accumulate over time and pose adverse effects on the health of the users. The results of the hazard quotient (HQ) and health risk index (HI) indicate there was no harmful effect on human health related to heavy metals present in lipstick. Whereas the results of the biological activity of the samples indicated that there was no bacterial growth in expensive samples, cheap samples were contaminated with some types of organisms; this indicates poor quality.

Keywords

• Cosmetics
• Heavy metals
• Toxicity effects
• Health risk assessment
• Biological activity.

Supporting Institution

Collage of science, University of Diyala, Iraq.

Project Number

1

References

1. 1. Balarastaghi S, Khashaiarmanesh Z, Makhdoumi P, Alavizadeh SH, Sameie Moghadam Z, Shirani K, Karimi G. Determination of toxic element levels (lead and cadmium) in commonly used cosmetic products in Iran. Toxin Reviews. 2018 Apr 3;37(2):117-22.
2. 2. Marinoa T, Iovinea A, Casellab P, Martinoc M, Chianesea S, Laroccac V, Musmarra D, Molino A. From Haematococcus pluvialis microalgae a powerful antioxidant for cosmetic applications. Chem. Eng. 2020;79:271-6.
3. 3. Sani A, Gaya MB, Abubakar FA. Determination of some heavy metals in selected cosmetic products sold in kano metropolis, Nigeria. Toxicology reports. 2016 Jan 1;3:866-9.
4. 4. Karanje PS, Doijad RC, Bhosale RR. Formulation and evaluation of herbal lipstick containing amaranthus cruentus linn. Int. J. Res. Anal. Rev. 2020 Mar;7:246-55.
5. 5. Gurrieri L, Drenten J. The feminist politics of choice: lipstick as a marketplace icon. Consumption Markets & Culture. 2021 May 4;24(3):225-40.
6. 6. Oklo AD, Enenche DE, Aondoakaa MA. Heavy metals in some lipstick products marketed in Makurdi metropolis, Benue state Nigeria. International Journal of Environment, Agriculture and Biotechnology. 2020 Mar 23;5(2).
7. 7. Shukri NM, Bashir NA, Shohaimi NA, Abdullah WN, Abdullah NH, Ab Halim AZ, Salleh NF. Assessment of permissible limits for heavy metals in various inspired and authentic lipsticks. Malaysian Journal of Chemistry. 2020.
8. 8. Batista ÉF, dos Santos Augusto A, Pereira-Filho ER. Chemometric evaluation of Cd, Co, Cr, Cu, Ni (inductively coupled plasma optical emission spectrometry) and Pb (graphite furnace atomic absorption spectrometry) concentrations in lipstick samples intended to be used by adults and children. Talanta. 2016 Apr 1;150:206-12.

9. 9. Parizi MG, Sedaghat Z, Mazloomi M, Fararouei M. Serum level of lead and cadmium is linked to facial cosmetics use among Iranian young women. Environmental Science and Pollution Research. 2021 Mar;28(11):13913-8.
10. 10. Chophi R, Sharma S, Sharma S, Singh R. Trends in the forensic analysis of cosmetic evidence. Forensic Chemistry. 2019 Jun 1;14:100165.
11. 11. Ayenimo JG, Yusuf AM, Adekunle AS, Makinde OW. Heavy metal exposure from personal care products. Bulletin of environmental contamination and toxicology. 2010 Jan;84(1):8-14.
12. 12. Volpe MG, Nazzaro M, Coppola R, Rapuano F, Aquino RP. Determination and assessments of selected heavy metals in eye shadow cosmetics from China, Italy, and USA. Microchemical Journal. 2012 Mar 1;101:65-9.
13. 13. Al-Saleh I, Al-Enazi S, Shinwari N. Assessment of lead in cosmetic products. Regulatory toxicology and pharmacology. 2009 Jul 1;54(2):105-13.
14. 14. Sainio EL, Jolanki R, Hakala E, Kanerva L. Metals and arsenic in eye shadows. Contact dermatitis. 2000 Jan;42(1):5-10
15. 15. Ullah H, Noreen S, Rehman A, Waseem A, Zubair S, Adnan M, Ahmad I. Comparative study of heavy metals content in cosmetic products of different countries marketed in Khyber Pakhtunkhwa, Pakistan. Arabian Journal of Chemistry. 2017 Jan 1;10(1):10-8.
16. 16. Abdulkareem EA, Abdulsattar JO, Abdulsattar BO. Iron (II) Determination in Lipstick Samples using Spectrophotometric and Microfluidic Paper-based Analytical Device (µPADs) Platform via Complexation Reaction with Iron Chelator 1, 10-phenanthroline: A Comparative Study. Baghdad Sci. J. 2022;19(2):355-67.
17. 17. Zakaria A, Ho YB. Heavy metals contamination in lipsticks and their associated health risks to lipstick consumers. Regulatory toxicology and pharmacology. 2015 Oct 1;73(1):191-5.
18. 18. Zainy FM. Heavy metals in lipstick products marketed in Saudi Arabia. Journal of Cosmetics, Dermatological Sciences and Applications. 2017 Nov 9;7(4):336-48.
19. 19. Sharafi K, Fatahi N, Yarmohammadi H, Moradi M, Dargahi A. Determination of cadmium and Lead concentrations in cosmetics (lipstick and hair color) in Kermanshah markets. Journal of Health. 2017 May 10;8(2):143-50.
20. 20. Copat C, Grasso A, Fiore M, Cristaldi A, Zuccarello P, Santo Signorelli S, Conti GO, Ferrante M. Trace elements in seafood from the Mediterranean sea: An exposure risk assessment. Food and chemical toxicology. 2018 May 1;115:13-9.
21. 21. Saadatzadeh A, Afzalan S, Zadehdabagh R, Tishezan L, Najafi N, Seyedtabib M, Noori SM. Determination of heavy metals (lead, cadmium, arsenic, and mercury) in authorized and unauthorized cosmetics. Cutaneous and ocular toxicology. 2019 Jul 3;38(3):207-11.
22. 22. Arshad H, Mehmood MZ, Shah MH, Abbasi AM. Evaluation of heavy metals in cosmetic products and their health risk assessment. Saudi Pharmaceutical Journal. 2020 Jul 1;28(7):779-90.
23. 23. Świerczek L, Cieślik B, Matysiak A, Konieczka P. Determination of heavy metals in eyeshadows from China. Monatshefte für Chemie-Chemical Monthly. 2019 Sep;150(9):1675-80.
24. 24. Ghaderpoori M, Kamarehie B, Jafari A, Alinejad AA, Hashempour Y, Saghi MH, Yousefi M, Oliveri Conti G, Mohammadi AA, Ghaderpoury A, Ferrante M. Health risk assessment of heavy metals in cosmetic products sold in Iran: the Monte Carlo simulation. Environmental Science and Pollution Research. 2020 Mar;27(7):7588-95.
25. 25. Abdulkareem EA, Abdulsattar JO. Determination of Nickel and Cobalt in Cosmetic Products Marketed in Iraq Using Spectrophotometric and Microfluidic Paper-based Analytical Device (µPADs) Platform. Baghdad Science Journal. 2022:1286-.
26. 26. Pawlaczyk A, Gajek M, Balcerek M, Szynkowska-Jóźwik MI. Determination of Metallic Impurities by ICP-MS Technique in Eyeshadows Purchased in Poland. Part I. Molecules. 2021 Nov 8;26(21):6753.
27. 27. Mrmošanin J, Pavlović A, Mitić S, Tošić S, Pecev-Marinković E, Krstić J, Nikolić M. The Evaluation of ICP OES for the Determination of Potentially Toxic Elements in Lipsticks: Health Risk Assessment. Acta Chimica Slovenica. 2019 Dec 18;66(4):802-13.
28. 28. Rehan I, Gondal MA, Rehan K, Sultana S, Khan S, Rehman MU, Waheed A, Salman SM. Nondestructive Determination of Chromium, Nickel, and Zinc in Neem Leaves and Facial Care Products by Laser Induced Breakdown Spectroscopy (LIBS). Analytical Letters. 2022 Apr 13;55(6):990-1003.
29. 29. Rawat K, Sharma N, Singh VK. X‐Ray Fluorescence and Comparison with Other Analytical Methods (AAS, ICP‐AES, LA‐ICP‐MS, IC, LIBS, SEM‐EDS, and XRD). X‐Ray Fluorescence in Biological Sciences: Principles, Instrumentation, and Applications. 2022 Apr 4:1-20.
30. 30. Kilic S, Kilic M, Soylak M. The determination of toxic metals in some traditional cosmetic products and health risk assessment. Biological Trace Element Research. 2021 Jun;199(6):2272-7.
31. 31. Zhong WS, Ren T, Zhao LJ. Determination of Pb (Lead), Cd (Cadmium), Cr (Chromium), Cu (Copper), and Ni (Nickel) in Chinese tea with high-resolution continuum source graphite furnace atomic absorption spectrometry. Journal of food and drug analysis. 2016 Jan 1;24(1):46-55.
32. 32. Fernández-Martínez R, Rucandio I, Gómez-Pinilla I, Borlaf F, García F, Larrea MT. Evaluation of different digestion systems for determination of trace mercury in seaweeds by cold vapour atomic fluorescence spectrometry. Journal of Food Composition and Analysis. 2015 Mar 1;38:7-12.
33. 33. Khalaf HS, Al-Haidari AM, Dikran SB, Mohammed AK. Spectrophotometric determination of sulfamethoxazole following simple diazotization and coupling with diphenylamine. Ibn AL-Haitham Journal For Pure and Applied Science. 2017 Apr 12;27(3):365-80.
34. 34. Kántor T, Bartha A, Ballók MI. Studies on chemical removal of excess nitric acid using formic acid in solutions analyzed by inductively coupled plasma optical emission spectrometry. Chemia analityczna. 2009 Jan 1;54(6):1265.
35. 35. El Haddad J, Canioni L, Bousquet B. Good practices in LIBS analysis: Review and advices. Spectrochimica Acta Part B: Atomic Spectroscopy. 2014 Nov 1;101:171-82.
36. 36. Ozer T, McMahon C, Henry CS. Advances in paper-based analytical devices. Annual Review of Analytical Chemistry. 2020 Jun 12;13:85-109.
37. 37. Lisowski P, Zarzycki PK. Microfluidic paper-based analytical devices (μPADs) and micro total analysis systems (μTAS): Development, applications and future trends. Chromatographia. 2013 Oct;76(19):1201-14.
38. 38. Alsaffar NM, Hussein HJ. Determination of heavy metals in some cosmetics available in locally markets. IOSR J Environ Sci Toxicol Food Technol. 2014;8(1):9-12.
39. 39. Nkansah MA, Owusu-Afriyie E, Opoku F. Determination of lead and cadmium contents in lipstick and their potential health risks to consumers. Journal of Consumer Protection and Food Safety. 2018 Dec;13(4):367-73.
40. 40. Naalbandi H, Saeedi M, Moharrami Moghanlou O, Akbari J, Morteza-Semnani K, Alizadeh R, Esfahani-Zadeh MH, Tajbakhsh M. Evaluation of heavy metal content of some lipsticks in Iran market. Pharmaceutical and Biomedical Research. 2016 Sep 10;2(3):31-7.
41. 41. Saleh FA, Saif RN, Murshed DM, Abdulmageed BA. Determination of Cadmium in some cosmetic products.
42. 42. Iwegbue CM, Bassey FI, Obi G, Tesi GO, Martincigh BS. Concentrations and exposure risks of some metals in facial cosmetics in Nigeria. Toxicology reports. 2016 Jan 1;3:464-72.
43. 43. Whitehouse L. Germany Reduces Heavy Metal Limits in Cosmetics.
44. 44. Alam MF, Akhter M, Mazumder B, Ferdous A, Hossain MD, Dafader NC, Ahmed FT, Kundu SK, Taheri T, Atique Ullah AK. Assessment of some heavy metals in selected cosmetics commonly used in Bangladesh and human health risk. Journal of Analytical Science and Technology. 2019 Dec;10(1):1-8.
45. 45. Schroeder HA. The poisons around us: toxic metals in food, air, and water.
46. 46. Franken A, Eloff FC, Du Plessis J, Du Plessis JL. In vitro permeation of metals through human skin: a review and recommendations. Chemical research in toxicology. 2015 Dec 21;28(12):2237-49.
47. 47. Ouremi OI, Ayodele OE. Lipsticks and nail polishes: potential sources of heavy metal in human body. Int J Pharm Res Allied Sci. 2014;3(4):45.
48. 48. Brzóska MM, Galażyn-Sidorczuk M, Borowska S. Metals in cosmetics. Metal Allergy: Springer; 2018. p. 177-96.
49. 49. Corazza M, Baldo F, Pagnoni A, Miscioscia R, Virgili A. Measurement of nickel, cobalt and chromium in toy make-up by atomic absorption spectroscopy. Acta Derm Venereol. 2009 Mar 1;89(2):130-3.
50. 50. Khalid A, Bukhari IH, Riaz M, Rehman G, Ain QU, Bokhari TH, Rasool N, Zubair M, Munir S. Determination of lead, cadmium, chromium, and nickel in different brands of lipsticks. International Journal of Biology, Pharmacy and Allied Sciences. 2013;1(2):263-71.
51. 51. Engwa GA, Ferdinand PU, Nwalo FN, Unachukwu MN. Mechanism and health effects of heavy metal toxicity in humans. Poisoning in the modern world-new tricks for an old dog. 2019 Jun 19;10:70-90.
52. 52. Cha NR, Lee JK, Lee YR, Jeong HJ, Kim HK, Lee SY. Determination of iron, copper, zinc, lead, nickel and cadmium in cosmetic matrices by flame atomic absorption spectroscopy. Analytical Letters. 2010 Jan 12;43(2):259-68.
53. 53. Wang B, Su Y, Tian L, Peng S, Ji R. Heavy metals in face paints: Assessment of the health risks to Chinese opera actors. Science of The Total Environment. 2020 Jul 1;724:138163.
54. 54. Wirth JJ, Mijal RS. Adverse effects of low level heavy metal exposure on male reproductive function. Systems biology in reproductive medicine. 2010 Jan 1;56(2):147-67.
55. 55. Lim DS, Roh TH, Kim MK, Kwon YC, Choi SM, Kwack SJ, Kim KB, Yoon S, Kim HS, Lee BM. Non-cancer, cancer, and dermal sensitization risk assessment of heavy metals in cosmetics. Journal of Toxicology and Environmental Health, Part A. 2018 Jun 3;81(11):432-52.
56. 56. Soares AR, Nascentes CC. Development of a simple method for the determination of lead in lipstick using alkaline solubilization and graphite furnace atomic absorption spectrometry. Talanta. 2013 Feb 15;105:272-7.
57. 57. Łodyga-Chruścińska E, Sykuła A, Więdłocha M. Hidden metals in several brands of lipstick and face powder present on polish market. Cosmetics. 2018 Oct 1;5(4):57.
58. 58. Bartzatt R. Neurological impact of zinc excess and deficiency in vivo. European Journal of Nutrition & Food Safety (ISSN: 2347-5641). 2017;7(3):155.
59. 59. Radfard M, Yunesian M, Nabizadeh R, Biglari H, Nazmara S, Hadi M, Yousefi N, Yousefi M, Abbasnia A, Mahvi AH. Drinking water quality and arsenic health risk assessment in Sistan and Baluchestan, Southeastern Province, Iran. Human and ecological risk assessment: An International Journal. 2018 May 7.
60. 60. Bilal M, Mehmood S, Iqbal HM. The beast of beauty: Environmental and health concerns of toxic components in cosmetics. Cosmetics. 2020 Feb 28;7(1):13.
61. 61. Yu S, Liao WT, Lee CH, Chai CY, Yu CL, Yu HS. Immunological dysfunction in chronic arsenic exposure: From subclinical condition to skin cancer. The Journal of dermatology. 2018 Nov;45(11):1271-7.
62. 62. Papadopoulos A, Assimomytis N, Varvaresou A. Sample Preparation of Cosmetic Products for the Determination of Heavy Metals. Cosmetics. 2022 Feb 4;9(1):21.
63. 63. Abd El-Aziz R, Abbassy MM, Hosny G. A comparative study on health risk Assessments of some heavy metals in cosmetics commonly used in Alexandria, Egypt. Int J Environ Sci Toxic Res. 2017;5(3):53-62.
64. 64. Parekh J, Chanda S. Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. African Journal of Biomedical Research. 2007;10(2).
65. 65. Mohammadi M, Riyahi Bakhtiari A, Khodabandeh S. Determination of cadmium and lead concentration in cosmetics (sunscreen, lipstick and hair color). Iranian Journal of Health and Environment. 2014 Mar 10;6(4):481-90.
66. 66. Ghaderpoori M, Kamarehie B, Jafari A, Alinejad AA, Hashempour Y, Saghi MH, Yousefi M, Oliveri Conti G, Mohammadi AA, Ghaderpoury A, Ferrante M. Health risk assessment of heavy metals in cosmetic products sold in Iran: the Monte Carlo simulation. Environmental Science and Pollution Research. 2020 Mar;27(7):7588-95.
67. 67. Malvandi H, Sancholi F. Assessments of some metals contamination in lipsticks and their associated health risks to lipstick consumers in Iran. Environmental monitoring and assessment. 2018 Nov;190(11):1-8.
68. 68. Pinto E, Paiva K, Carvalhido A, Almeida A. Elemental impurities in lipsticks: results from a survey of the Portuguese and Brazilian markets. Regulatory Toxicology and Pharmacology. 2018 Jun 1;95:307-13.
69. 69. Chauhan SB, Chandak A, Agrawal SS. Evaluation of heavy metals contamination in marketed lipsticks. International Journal of Advanced Research. 2014;2(4):257-62.
70. 70. Nourmoradi H, Foroghi M, Farhadkhani M, Vahid Dastjerdi M. Assessment of lead and cadmium levels in frequently used cosmetic products in Iran. Journal of environmental and public health. 2013 Jan 1;2013.
71. 71. Benzeid H, Mojemmi B, Chentoufi MA, Benabbes M, Rahali Y, Cheikh A, Touré HA, Draoui M. Determination of Lead and Cadmium in Synthetic and Natural Hair Dyes in Morocco Using Differential Pulse Polarography. Portugaliae Electrochimica Acta. 2021;39(1):37-44.
72. 72. Ghaderpoori M, Kamarehie B, Jafari A, Alinejad AA, Hashempour Y, Saghi MH, Yousefi M, Oliveri Conti G, Mohammadi AA, Ghaderpoury A, Ferrante M. Health risk assessment of heavy metals in cosmetic products sold in Iran: the Monte Carlo simulation. Environmental Science and Pollution Research. 2020 Mar;27(7):7588-95.
73. 73. Munir A, Hayyat MU, Shahzad L, Sharif F, Farhan M, Ghafoor GZ. Assessment of heavy metals concentrations in commercially available lipsticks in Pakistan. Environmental Forensics. 2020 Nov 7;21(3-4):259-66.
74. 74. Nasirudeen MB, Amaechi AU. Spectrophotometric determination of heavy metals in cosmetics sourced from Kaduna Metropolis, Nigeria. Science World Journal. 2015;10(3):1-5.
75. 75. Kilic S, Kilic M, Soylak M. The determination of toxic metals in some traditional cosmetic products and health risk assessment. Biological Trace Element Research. 2021 Jun;199(6):2272-7.
76. 76. Lee BM, Kwon S, Cho YM, Kim KB, Seo K, Min CS, Kim K. Perspectives on trace chemical safety and chemophobia: risk communication and risk management. Journal of Toxicology and Environmental Health, Part A. 2019 Feb 1;82(3):186-99.
77. 77. Stewart S, Parker M, Amézquita Stewart SE, Parker MD, Amézquita A, Pitt TL. Microbiological risk assessment for personal care products. International journal of cosmetic science. 2016 Dec;38(6):634-45. Stewart SE, Parker MD, Amézquita A, Pitt TL. Microbiological risk assessment for personal care products. International journal of cosmetic science. 2016 Dec;38(6):634-45.
78. 78. Siya K, Thomas J, Kumar RV, Saji AM, Iype AK, Akhil S. Lipsticks: The microbial cellar: An original study. Journal of microscopy and ultrastructure. 2019 Oct;7(4):194.
79. 79. Vassoler M, Tonial F, Fagundes SC, Fagundes MA, Zortéa NB, Rossato-Grando LG, Bertol CD. Microbiological Contamination of In-Store Lipstick Testers Available to the Consumer.