Preparation and in Vitro Adhesive Application of Visible Light-Activated Modified Sodium Alginate

Abstract

Materials that are responsive to visible light have been extensively used in biomedical applications, including tissue engineering. Tissue adhesives are among the most important applications of tissue engineering. In this study, different concentrations of Ruthenium (Ru) and sodium persulfate (SPS) photoinitiators (0.2/2, 0.5/5, and 1/10 mM) were prepared. Sodium alginate (Na-alginate) was modified with methacrylate (AlgMA) to render it photoactive. Photoactive materials prepared with different ratios of photoinitiators were physically, chemically, morphologically, and mechanically tested. The results of the different analyses supported each other. Ru/SPS concentrations of 0.2/2, 0.5/5, and 1/10 mM were used to evaluate the hydrogel structures. No physical, chemical, or mechanical differences were observed between the 0.5/5, and 1/10 mM. The in vitro adhesion properties of the hydrogels increased significantly from 0.2/2 mM to 1/10 mM Ru/SPS. As the Ru/SPS concentration was increased, a significant increase in cell viability was observed. In particular, 1/10 mM Ru/SPS showed the greatest effect and created the most statistically significant difference.

Keywords

• AlgMA
• Ru/SPS
• Light-activation
• visible light
• Tissue adhesives

References

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