The Effects of Thiosemicarbazone-based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues

Refiye Yanardağ; Onur Ertik; Tülay Bal Demirci; Bahri Ülküseven; Sevim Tunalı

doi:10.18596/jotcsa.1734840

The Effects of Thiosemicarbazone-based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues

Abstract

A vanadium compound, 2,4-dihydroxybenzylidene-N(4)-2-hydroxybenzylidene-S-methyl-isothiosemicarbazidato-oxidovanadium(IV) (VOL), was investigated for its possible benefits in the treatment of diabetes-related symptoms. Male Swiss albino rats aged 3 to 3.5 months were used in the study. The animals were randomly assigned to four groups. Experimental diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ) at a dose of 65 mg/kg. The groups were as follows: Group I – healthy control (no treatment); Group II – healthy control rats administered VOL; Group III – STZ-induced diabetic rats; Group IV – STZ-induced diabetic rats treated with VOL. After diabetes was induced, VOL was administered to the rats in Groups II and IV via gavage at a daily dose of 0.2 mM/kg for 12 consecutive days. Based on biochemical results, in lens and skin tissues, reduced glutathione levels, catalase, and superoxide dismutase activities were increased, whereas lipid peroxidation and non-enzymatic glycosylated levels were decreased in VOL-treated diabetic rats. Besides that, enzyme activities in the polyol pathway decreased in the lens tissues of diabetic animals given VOL. The binding affinities of these two enzymes (AR and SDH) to VOL were also investigated using molecular docking based on the conformational state. The results revealed that the use of VOL can be effective in preventing or at least retarding the development of some diabetic ocular and dermal complications.

Keywords

Project Number

Research Fund of Istanbul University financed this study (Project No: BYP/3864 and Project No: BYP/37135).

Ethical Statement

All experimental stages in the study were reviewed and approved by the Istanbul University Animal Care and Use Committee.

Thanks

Research Fund of Istanbul University financed this study (Project No: BYP/3864 and Project No: BYP/37135).

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Refiye Yanardağ ^*
0000-0003-4185-4363
Türkiye

Onur Ertik
0000-0001-5437-3513
Türkiye

Tülay Bal Demirci
0000-0003-4663-2209
Türkiye

Bahri Ülküseven
0000-0001-6342-1505
Türkiye

Sevim Tunalı
0000-0003-3363-1290
Türkiye

Publication Date

December 1, 2025

Submission Date

July 5, 2025

Acceptance Date

October 13, 2025

Published in Issue

Year 2025 Volume: 12 Number: 4

DOI

https://doi.org/10.18596/jotcsa.1734840

IZ

https://izlik.org/JA29EB59BS

Cite

RIS / Bibtex

APA

Yanardağ, R., Ertik, O., Demirci, T. B., Ülküseven, B., & Tunalı, S. (2025). The Effects of Thiosemicarbazone-based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues. Journal of the Turkish Chemical Society Section A: Chemistry, 12(4), 221-234. https://doi.org/10.18596/jotcsa.1734840

AMA

1.Yanardağ R, Ertik O, Demirci TB, Ülküseven B, Tunalı S. The Effects of Thiosemicarbazone-based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues. JOTCSA. 2025;12(4):221-234. doi:10.18596/jotcsa.1734840

Chicago

Yanardağ, Refiye, Onur Ertik, Tülay Bal Demirci, Bahri Ülküseven, and Sevim Tunalı. 2025. “The Effects of Thiosemicarbazone-Based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues”. Journal of the Turkish Chemical Society Section A: Chemistry 12 (4): 221-34. https://doi.org/10.18596/jotcsa.1734840.

EndNote

Yanardağ R, Ertik O, Demirci TB, Ülküseven B, Tunalı S (December 1, 2025) The Effects of Thiosemicarbazone-based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues. Journal of the Turkish Chemical Society Section A: Chemistry 12 4 221–234.

IEEE

[1]R. Yanardağ, O. Ertik, T. B. Demirci, B. Ülküseven, and S. Tunalı, “The Effects of Thiosemicarbazone-based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues”, JOTCSA, vol. 12, no. 4, pp. 221–234, Dec. 2025, doi: 10.18596/jotcsa.1734840.

ISNAD

Yanardağ, Refiye - Ertik, Onur - Demirci, Tülay Bal - Ülküseven, Bahri - Tunalı, Sevim. “The Effects of Thiosemicarbazone-Based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues”. Journal of the Turkish Chemical Society Section A: Chemistry 12/4 (December 1, 2025): 221-234. https://doi.org/10.18596/jotcsa.1734840.

JAMA

1.Yanardağ R, Ertik O, Demirci TB, Ülküseven B, Tunalı S. The Effects of Thiosemicarbazone-based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues. JOTCSA. 2025;12:221–234.

MLA

Yanardağ, Refiye, et al. “The Effects of Thiosemicarbazone-Based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues”. Journal of the Turkish Chemical Society Section A: Chemistry, vol. 12, no. 4, Dec. 2025, pp. 221-34, doi:10.18596/jotcsa.1734840.

Vancouver

1.Refiye Yanardağ, Onur Ertik, Tülay Bal Demirci, Bahri Ülküseven, Sevim Tunalı. The Effects of Thiosemicarbazone-based Oxovanadium (IV) Complex on the Lens and Skin Tissues in Streptozotocin-Induced Diabetic Rats and Computational Studies for the Key Target Proteins of the Lens Tissues. JOTCSA. 2025 Dec. 1;12(4):221-34. doi:10.18596/jotcsa.1734840