Phytochemical Profiling and Evaluation of Marrubium sivasense Aytaç, Akgül & Ekici for Antioxidant Activity and Inhibition Effects on α-Amylase, Lipoxygenase, Xanthine Oxidase and Tyrosinase Enzymes

Abstract

Methanol extract of Marrubium. sivasense Aytaç, Akgül & Ekici was analyzed with LC-MS/MS technique. Forsythoside B, verbascoside and leucoseptoside were identified as main compounds. Antioxidant properties of the extract was determined by DPPH free radical scavenging activity (IC50 0.34 mg/mL), trolox equivalent antioxidant capacity (TEAC) (2.3 mM±0.04), xanthine-xanthine oxidase(XO) inhibitory effect (16.54 %). and. B-carotene bleaching (Inh. 43±1.26 %) assays. The extract demonstrated hypoglycemic activity (Inh. 49.67±1.09 %.) via inhibition of porcine pancreatic α-amylase, the antiinflammatory effect of the extract tested via inhibition of 5-LOX was found as Inh 18.71±0.74 %, however no antityrosinase activity was found. 

Keywords

• Marrubium sivasense
• extract
• LC-MS/MS
• antioxidant
• α-Amylase
• xanthine oxidase
• tyrosinase

Supporting Institution

Anadolu University Scientific Research Department

Project Number

1705S171

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