Antibiotic Susceptibility Of Klebsiella pneumoniae Strains Isolated From Clinical Samples

Year 2020, , 319 - 326, 15.02.2020

Ahmet Ozan Özgen , Ozan Emre Eyüpoğlu

https://doi.org/10.18596/jotcsa.635088

Cited By: 2

Abstract

Antibiotic
resistance in bacteria has become a worrying phenomenon in today's world. K. pneumoniae is a member of the Enterobacteriaceae family, which causes
nosocomial infections as an opportunistic pathogen but inherently harboured as
a part of natural human microbiota. Carbapenem resistance of K. pneumoniae was a rare occurrence up
to ten years ago, but in recent years many types of carbapenemase producing K. pneumoniae have become common. The
aim of this retrospective study; analyzing susceptibility to various
antibiotics, commonly used in treatment, against K. pneumoniae strains isolated from various infection sites.
Antibiotic susceptibility tests were performed by using the VITEK 2 Compact ®
automated system. In this study, 502 K.
pneumoniae strains  isolated from
patients that treated at various services of a university hospital with 515 bed
capacity were examined. When compared to the data available with studies of
recent years in Turkey, especially in intensive care and inpatient services,
resistance of K. pneumoniae strains
to antibiotics, against most carbapenems, are rapidly increasing in the degree
of high concern. Therefore, in all hospitals, antibiotic management policies
should be implemented with a multidisciplinary approach.

Keywords

Enterobacteriaceae, Klebsiella pneumoniae, Drug Resistance, Bacterial, Retrospective Studies

Supporting Institution

İstanbul Medipol University

Thanks

The authors would like to thank Dr. Betül GİRAY for his help in supplying research materials and Ph.D. Meltem Ezgi DURGUN for her help in statistical data analysis. We also appreciate the support from the management of Clinical Microbiology Laboratory, Medipol University Hospital.

References

• 1. Sirijatuphat R, Sripanidkulchai K, Boonyasiri A, Rattanaumpawan P, Supapueng O, Kiratisin P, et al. Implementation of global antimicrobial resistance surveillance system (GLASS) in patients with bacteremia. PLOS ONE. 2018 Oca;13(1):e0190132.
• 2. Leung E, Weil DE, Raviglione M, Nakatani H. The WHO policy package to combat antimicrobial resistance. Bull World Health Organ. 2011 May;89:390–2.
• 3. Reçetesiz ilaç satılmıyor [Internet]. CNN Türk. [cited 2020 Jan 20]. Available from: https://www.cnnturk.com/turkiye/recetesiz-ilac-satilmiyor
• 4. Murray PR. Aerobic Fermentative Gram-Negative Rods. In: Basic Medical Microbiology. Philadelphia, USA: Elsevier; 2018. p. 85–9.
• 5. Paterson DL. Resistance in gram-negative bacteria: Enterobacteriaceae. Am J Infect Control. 2006 Jun 1;34(5, Supplement):S20–8.
• 6. Bassetti M, Giacobbe DR, Giamarellou H, Viscoli C, Daikos GL, Dimopoulos G, et al. Management of KPC-producing Klebsiella pneumoniae infections. Clin Microbiol Infect. 2018 Feb;24(2):133–44.
• 7. Giakkoupi P, Xanthaki A, Kanelopoulou M, Vlahaki A, Miriagou V, Kontou S, et al. VIM-1 Metallo- -Lactamase-Producing Klebsiella pneumoniae Strains in Greek Hospitals. J Clin Microbiol. 2003 Aug 1;41(8):3893–6.
• 8. Pereira EC, Shaw KM, Vagnone PMS, Harper J, Lynfield R. A review of multidrug-resistant Enterobacteriaceae. Minn Med. 2011 Oct;94(10):44–8.
• 9. Leclercq R, Cantón R, Brown DFJ, Giske CG, Heisig P, MacGowan AP, et al. EUCAST expert rules in antimicrobial susceptibility testing. Clin Microbiol Infect. 2013 Feb 1;19(2):141–60.
• 10. Liu C, Guo J. Hypervirulent Klebsiella pneumoniae (hypermucoviscous and aerobactin positive) infection over 6 years in the elderly in China: antimicrobial resistance patterns, molecular epidemiology and risk factor. Ann Clin Microbiol Antimicrob. 2019 Jan 21;18(1):4.
• 11. Dasgupta S, Das S, Chawan NS, Hazra A. Nosocomial infections in the intensive care unit: Incidence, risk factors, outcome and associated pathogens in a public tertiary teaching hospital of Eastern India. Indian J Crit Care Med Peer-Rev Off Publ Indian Soc Crit Care Med. 2015 Jan;19(1):14–20.
• 12. Taitt CR, Leski TA, Erwin DP, Odundo EA, Kipkemoi NC, Ndonye JN, et al. Antimicrobial resistance of Klebsiella pneumoniae stool isolates circulating in Kenya. Butaye P, editor. PLOS ONE. 2017 Jun 2;12(6):e0178880.
• 13. Moghnieh R, Estaitieh N, Mugharbil A, Jisr T, Abdallah DI, Ziade F, et al. Third generation cephalosporin resistant Enterobacteriaceae and multidrug resistant gram-negative bacteria causing bacteremia in febrile neutropenia adult cancer patients in Lebanon, broad spectrum antibiotics use as a major risk factor, and correlation with poor prognosis. Front Cell Infect Microbiol [Internet]. 2015 Feb 12 [cited 2020 Jan 19];5. Available from: http://journal.frontiersin.org/Article/10.3389/fcimb.2015.00011/abstract
• 14. Shao Y, Lu R, Yang Y, Xu Q, Wang B, Ye G. Antibiotic resistance of Helicobacter pylori to 16 antibiotics in clinical patients. J Clin Lab Anal. 2018 May;32(4):e22339.
• 15. Liu D-S, Wang Y-H, Zeng Z-R, Zhang Z-Y, Lu H, Xu J-M, et al. Primary antibiotic resistance of Helicobacter pylori in Chinese patients: a multiregion prospective 7-year study. Clin Microbiol Infect. 2018 Jul;24(7):780.e5-780.e8.
• 16. Sainani KL. Clinical Versus Statistical Significance. PM&R. 2012;4(6):442–5.
• 17. Aykan ŞB, Çi̇Ftci̇ İH. Türkiye’de İdrar Kültürlerinden İzole Edilen Escherichia coli Suşlarının Antibiyotiklere Direnç Durumu: Bir Meta-Analiz. :16.
• 18. Hardin M, Foreman M, Dransfield MT, Hansel N, Han MK, Cho MH, et al. Sex-specific features of emphysema among current and former smokers with COPD. Eur Respir J. 2016 Jan 1;47(1):104–12.
• 19. Fethers KA, Fairley CK, Hocking JS, Gurrin LC, Bradshaw CS. Sexual Risk Factors and Bacterial Vaginosis: A Systematic Review and Meta-Analysis. Clin Infect Dis. 2008 Dec 1;47(11):1426–35.
• 20. Balikçi H, Açikgöz ZC, Güvenman S, Çel N, Özdem B. Escherichia coli ve Klebsiella spp. İzolatlarında Plazmid Kaynaklı AmpC Beta-Laktamaz Üretiminin Araştırılması. MİKROBİYOLOJİ Bül. :13.
• 21. Eraksoy H, Basustaoglu A, Korten V, Kurt H, Ozturk R, Ulusoy S, et al. Susceptibility of Bacterial Isolates From Turkey - A Report From the Meropenem Yearly Susceptibility Test Information Collection (MYSTIC) Program. J Chemother. 2007 Dec 1;19(6):650–7.
• 22. Kahraman EP, Karakeçe E, Erdoğan F, Uluyurt H, Köroğlu M, Çiftci İH. Klebsiella pneumoniae izolatlarının antibiyotiklere direnç durumlarının değerlendirilmesi. Ortadoğu Tıp Derg. 2017 Mar 2;9(1):12–8.
• 23. Al-Muhtaseb M, Kaygusuz A. Kan kültürlerinden izole edilen Escherichia coli ve Klebsiella pneumoniae suşlarında genişletilmiş spektrumlu betalaktamaz (GSBL) sıklığı. ANKEM Dergi. 2008;22(4):175–82.
• 24. Samasti M, Koçoğlu ME, Davarci İ, Vahaboğlu H, Çaşkurlu H. Investigation of Carbapenemase Genes and Clonal Relationship in Carbapenem Resistant Klebsiella pneumoniae Strains. Bezmialem Sci. 2019 Jul 26;7(3):186–90.
• 25. Darby L. Antibiotic-Resistant Plagues Are Coming and We’re Wildly Unprepared [Internet]. [cited 2019 Oct 20]. Available from: https://www.gq.com/story/antibiotic-resistant-plagues-are-coming