A versatile water soluble ball-type phthalocyanine as potential antiproliferative drug: the interaction with G-quadruplex formed from Tel 21 and cMYC

Year 2017, Volume: 4 Issue: 2, 579 - 596, 02.05.2017

Efkan Bağda , Esra Bağda , Ebru Yabaş

https://doi.org/10.18596/jotcsa.288284

Abstract

G-quadruplexes are biologically
important DNA conformations exist generally in guanine rich segments of DNA,
such as telomere and proto-oncogene.
The formation of
these secondary structures is thought to inhibit the expression of certain
genes, such as the inhibition of telomerase. The
inhibition of telomerase and suppression of a specific gene expression are
important approaches for interruption of cancer cell's proliferation. In the
present study, the effect of a versatile water soluble ball-type phthalocyanine
on G-quadruplex formation and stabilization was investigated to demonstrate its
potential usage in cancer chemotherapy. Two important guanine rich oligomers,
cMYC and Tel 21 were used as G-quadruplex former sequence. To the best of our
knowledge, this is the first study about the interaction of a ball-type
phthalocyanine with G-quadruplex structures. 
The interactions of the compound with G-quadruplex molecules were
monitored spectrophotometrically. The structural differentiations of
G-quadruplex after binding were investigated with circular dichroism
spectrophotometry. The replacement ability of the molecule with a known DNA
binding molecule, ethidium bromide, was clarified fluorometrically. The
Stern-Volmer studies were conducted for determination of the quenching
mechanism. The strong interaction of the molecules (ball-type phthalocyanine
with oligomer) showed us the potential usage of these drug conjugates for
targeted photodynamic therapy in the future. 

Keywords

G-quadruplex, CD spectroscopic titration, telomere, oncogene

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