Antiproliferative effect of Cherry laurel

Yıl 2016, Cilt: 3 Sayı: 3, 217 - 228, 08.01.2017

Ali Aydin , Ramazan Erenler , Bülent Yılmaz Şaban Tekin

https://doi.org/10.18596/jotcsa.21204

Cited By: 16

Öz

Cherry laurel (Prunus laurocerasus) fruits including phenolic and flavonoid contents are consumed as fresh, dried or prepared in marmalade and its leaves containing cyanogenic glycosides have healing activity of a well-known Anatolian folkloric remedy. Herein, Cherry laurel fruits were boiled in water for 2 h then extracted with hexane, EtOAc and BuOH successively. Antiproliferative activities of extracts were evaluated on HeLa (Human Cervix Carcinoma), HT29 (Human Colorectal Adenocarcinoma), C6 (Rat Brain Tumor Cells), and Vero (African Green Monkey Kidney) cell lines. All extracts exhibited slightly antiproliferative effects on various cancer cell lines at high concentration. We assessed the ability of extracts of cherry laurel fruit to devastate the membrane of cells. Results indicated that cherry laurel fruit extracts slightly destroys the cellular membrane in tumor cell lines at high concentration merely. The results of this study not support the efficacy of cherry laurel fruit extracts as an anticancer agent for cancer cells, but it suggests that cherry laurel fruit extracts may be used through reducing cytotoxicity a potential adjuvant therapy to current chemotherapeutic agents. Fatty acids of hexane extract were also determined by GC-MS analysis and found out that linoleic acid, palmitic acid and oleic acid were the major products. 

Kaynakça

• Elgorashi E. Screening of medicinal plants used in South African traditional medicine for genotoxic effects. Toxicol. Lett. 2003; 143, 195–207. doi:10.1016/S0378-4274(03)00176-0.
• Sithranga Boopathy N, Kathiresan K. Anticancer Drugs from Marine Flora: An Overview. J. Oncol. 2010; 1–18. doi:10.1155/2010/214186.
• Lee CC, Houghton P. Cytotoxicity of plants from Malaysia and Thailand used traditionally to treat cancer. J. Ethnopharmacol. 2005; 100, 237–243.
• Itharat A, Houghton PJ, Eno-Amooquaye E, Burke P, Sampson JH, Raman A. In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer. J. Ethnopharmacol. 2004; 90, 33–38.
• Elmastas M, Genc N, Demirtas I, Aksit H, Aboul-Enien HY. Isolation and Identification of Functional Components in Seed of Cherry Laurel (Laurocerasus officinalis Roem.) and Investigation of Their Antioxidant Capacity. J. Biol. Act. Prod. from Nat. 2013; 3, 115–120. doi:10.1080/22311866.2013.817736.
• Karabegović IT, Stojičević SS, Veličković DT, Todorović ZB, Nikolić NČ, Lazić ML. The effect of different extraction techniques on the composition and antioxidant activity of cherry laurel (Prunus laurocerasus) leaf and fruit extracts. Ind. Crops Prod. 2014; 54, 142–148. doi:10.1016/j.indcrop.2013.12.047.
• Türkan K, Kasim T, Bircan E, Murat K. DNA damage protecting activity and in vitro antioxidant potential of the methanol extract of Cherry (Prunus avium L). J. Med. Plants Res. 2014; 8, 715–726. doi:10.5897/JMPR2013.5350.
• Akkol EK, Kırmızıbekmez H, Kuçukboyacı N, Goren AC, Yesilada E. Isolation of active constituents from cherry laurel (Laurocerasus officinalis Roem.) leaves through bioassay-guided procedures. J. Ethnopharmacol. 2012; 139, 527–532.
• Alasalvar C, Al-Farsi M, Shahidi F. Compositional characteristics and antioxidant components of cherry laurel varieties and pekmez. J. Food Sci. 2005; 70(1), S47–S52.
• Ayaz FA, Kadioglu A, Reunanen M, Var M. Phenolic acid and fatty acid composition in the fruits of Laurocerasus officinalis Roem. and its cultivars. J. Food Compos. Anal. 1997; 10(4), 350–357.
• Karahalil FY, Sahin H. Phenolic composition and antioxidant capacity of cherry laurel (Laurocerasus officinalis Roem.) sampled from Trabzon region, Turkey. Afr. J. Biotechnol. 2011; 10(72), 16293–16299.
• Kolayli S, Kuçuk M, Duran C, Candan F, Dinçer B. Chemical and antioxidant properties of Laurocerasus officinalis Roem. (cherry laurel) fruit grown in the Black Sea region. J. Agric. Food Chem. 2003; 51(25), 7489–7494.
• Sahan Y, Cansev A, Celik G, Cinar A. Determination of various chemical properties, total phenolic contents, antioxidant capacity and organic acids in Laurocerasus officinalis fruits. Acta Hortic. 2012; 939, 359–366.
• Porstmann T, Ternynck T, Avrameas S. Quantitation of 5-bromo-2-deoxyuridine incorporation into DNA: an enzyme immunoassay for the assessment of the lymphoid cell proliferative response. J Immunol Methods 1985; 82(1): 169-79. doi:10.1016/0022-1759(85)90236-4.
• Weerapreeyakul N, Nonpunya A, Barusrux S, Thitimetharoch T, Sripanidkulchai B. Evaluation of the anticancer potential of six herbs against a hepatoma cell line. Chin. Med. 2012; 7, 15.
• Zheng S-Y. Anticancer effect and apoptosis induction by quercetin in the human lung cancer cell line A-549. Mol. Med. Rep. 2011.
• Niu G, Yin S, Xie S, Li Y, Nie D, Ma L, Wang X, Wu Y. Quercetin induces apoptosis by activating caspase-3 and regulating Bcl-2 and cyclooxygenase-2 pathways in human HL-60 cells. Acta Biochim. Biophys. Sin. (Shanghai). 2011; 43, 30–37.
• Ferry DR, Smith A, Malkhandi J, Fyfe DW, DeTakats PG, Anderson D, Baker J, Kerr J. Phase I clinical trial of the flavonoid quercetin: pharmacokinetics and evidence for in vivo tyrosine kinase inhibition. Clin. Cancer Res. 1996; 2, 659–68.
• Mandal AK, Das S, Mitra M, Chakrabarti RN, Chatterjee M, Das N. Vesicular flavonoid in combating diethylnitrosamine induced hepatocarcinoma in rat model. J Exp Ther Oncol 2008; 7, 123-133.
• C.T.E.B.PDQ Integrative, Alternative, Laetrile/Amygdalin (PDQ®): Health Professional Version. 2002.
• Halilova H, Ercisli S. Several Physico-Chemical Characteristics of Cherry Laurel (Laurocerasos Officinalis Roem.) Fruits. Biotechnol. Biotechnol. Equip. 2010; 24, 1970–1973.
• Stanisavljević IT, Lazić ML, Veljković VB, Stojičević SS, Veličković DT, Ristić MS. Kinetics of Hydrodistillation and Chemical Composition of Essential Oil from Cherry Laurel (Prunus laurocerasus L. var. serbica Pančić) Leaves. J. Essent. Oil Res. 2010; 22, 564–567.