ANTI-DIABETIC EFFICACY AND PHYTOCHEMICAL SCREENING OF METHANOLIC LEAF EXTRACT OF PAWPAW (Carica papaya) GROWN IN NORTH CENTRAL NIGERIA.

Year 2017, Volume: 4 Issue: 1, 99 - 114, 09.01.2017

Ayorinde Victor Ogundele Kabir Opeyemi Otun Abdulfatai Ajiboye , Bolatito Eunice Olanipekun Rasheed Bolaji Ibrahim

https://doi.org/10.18596/jotcsa.52813

Cited By: 3

Abstract

Carica papaya leaves samples (Green) were freshly harvested from Islamic village in Ilorin, Ilorin west local Government, Kwara State Nigeria. The leaves were extracted with methanol; the resulting extracts were screened for the phytochemical constituents using standard procedure. Phytochemical screening revealed the presence of bioactive compounds such as tannins, saponins, terpenoids, glycosides and alkaloids. The in-vitro anti-diabetic potential of the plant was also determined so as to justify the traditional usage of the plant in treating diabetes. The result of the present study confirmed that the methanolic extract of C.papaya leaves possess significant anti-diabetic activity in-vitro, this shows that the leaves has the potential for the development of drugs in combating diabetes.

Keywords

carica papaya leaves, phytochemical screening, bioactive, anti-diabetic, in-vitro

References

• World Health Forum; Controller of Publication, United States of America, Ministry of Health Sciences forum, WHO, 1993;14(6): 390-396
• Narkhede, M.B., Ajimire, P.V., Wagh, A.E., Manoj, M. and Shivashanmugam, A.T. In vitro antidiabetic activity of Caesalpina digyna (R.) methanol root extract. Asian J. Plant Sci. Res., 2011; 1(2): 101-106
• Polonsky, K.S.,. The past 200 years in diabetes. N. Engl. J. Med. 2012; 367, 1332-1340.
• DK Patel, SK Prasad, R Kumar, S Hemalatha.. An overview on antidiabetic medicinal plants having insulin mimetics property Asian Pac J Trop Biomed, 2012; 2 (4), pp. 320–330
• Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK. Anti-diabetic agents from medicinal plants. Curr med chem. 2006; 13(10): 1203-1218.
• Modak M, Dixit P, Londhe J, Ghaskadbi S, Paul A, Devasagayam T. Indian herbs and herbal drugs used for the treatment of diabetes. J. Clin Biochem Nutri. 2007; 40 (3): 163-173.
• Jarald E, Joshi SB, Jain DC. Diabetes and herbal medicines. Iran J Pharmacol Ther. 2008;7(1): 97-106.
• Simon JE (1995). Basil New crop Factsheet Centre for New Crops and Plant Products, Purdue University
• Grover JK, Yadav S, Vats V. Medicinal plants of india with anti-diabetic potential. J Ethnopharmacol. 2002; 81: 81-100
• Brai BIC, Odetola AA, Agomo PU. Anti-hyperglycemic activity of Cassia kleinii leaf extract in normal rats and alloxan-induced diabetic rats. Indian J. Pharmcol. 2007; 34: 409-415.
• Gondwe M, Okoro. PK, Juta R. Effect of diabetes on kidney. Diabetes.2008; 52: 283-291
• Ojiako AO, Chikezie PC, Ogbuji CA (2015) Blood glucose level and lipid profile of alloxan-induced hyperglycaemic rats treated with single and combinatorial herbal formulations J.Tradit Compl
• Ojiako AO, Chikezie PC. Comparative proximate composition and hypoglycaemic properties of three medicinal plants (Verononia amygdalina, Azadirachata indica and Moringa oleifera). Pharmacogn commun .2014;4: 40-48.
• Ugochukwu NH, Fafunso PJ, Boba AT, Babady NE. The various medicinal effects of Gongronema latifolium. Phytother Res.2005;12:46-52,
• Ojewole JAO. Analgesic, anti-inflammatory and hypoglycaemic effects of Securidaca longepedunculata (Fresen.) [Polygalaceae] root-bark aqueous extracts inflammo-pharmacol. 2008;16: 174-181
• Ibegbulem CO, Chikezie PC. Hypoglycaemic properties of ethanolic extracts of Gongronema latifolium, Aloe perryi, Viscum album and Allium sativum administered to alloxan-induced diabetic albino rats (Rattus norvegicus). Pharmacogn commun.2013;3:12-16
• Chikezie PC, Uwakwe AA. Activities of three erythrocyte enzymes of hyperglycaemic rats (Rattus norvegicus) treated with Allium sativa extract. J Diabetes Meta Discord.2014;13:50.
• Sathasivam K, Ramanathan S, Mansor S, Haris M, Wernsdorfer W.“Thrombocytecount in mice after administration of papaya leaf suspension”, The Middle European Journal of Medicine, 2009; 121(3), 19-22.).
• Yogiraj Vijay, Pradeep Kumar Goyal, Chetan Singh Chauhan, Anju Goyal, Bhupendra Vyas. Carica papaya Linn: An Overview. International Journal of Herbal Medicine, 2014;2 (5): 01-08
• Adeneye, A.A., & Olagunju, I.A. Preliminary hypoglycemic and hypolipidemic activities of the aqueous seed extract of Carica papaya Linn. in Wistar rats. Biology and Medicine.2009;1:1–10
• Ahmad, N., Fazal, H., Ayaz, M., Abbasi, B.H., Mohammad, I., Fazal, L.. Dengue fever treatment with Carica papaya leaves extracts. Asian Pac. J. Trop. Biomed.2011;1:330-333
• Owoyele, B.V., Adebukola, O.M., Funmilayo, A.A., Soladoye, A.O. Anti-inflammatory activities of ethanolic extract of Carica papaya leaves. Inflammopharmacol. 2008;16: 168-173.
• Aruoma, O.I., Hayashi, Y., Marotta, F., Mantello, P., Rachmilewitz, E., Montagnier, L. Applications and bioefficacy of the functional food supplement fermented papaya preparation. Toxicology. 2010;278: 6-16
• William PJ, Douglas K. Extraction of plant secondary metabolites in natural products isolation. 2nd ed. Humana Press, New Jersey.2006;20: 323-351
• Gupta D, Kondongala S.C, Girish P. In vitro antidiabetic activity of pentacyclic tritrprnoida and fatty acid ester from bauhinia purpurea. Inter. J. Pharmacol. Pharmaceut. Tech. ISSN-2277- 3436.2013;Vol-2, Iss-1,
• Shekib L.A, Iraq E.I, Abo Baker S.M. "Studies on amylase inhibitors in some Egyptian legume seeds" Plant Foods Human Nutr; 1988;38, 325–332.).
• Rajesh P. Dinesh Kumar Damiki Laloo, Siva Hemalatha. Evaluation of phytochemical and antioxidant activities of the different fractions of Hybanthus enneaspermus Linn F Muell Violaceae. Asian Pacific J of Tropical Medicine: 2009;39 :1-6.)
• Akhila S., and N. G. Vijayalakshmi., “Phytochemical Studies On Carica Papaya Leaf Juice”, IJPSR, 2015; 6 (2), 880883.
• Sheikh Fauziya and R Krishnamurthy. “Papaya (carica papaya): source material for anticancer”, CIBTech Journal of Pharmaceutical Sciences, 2013;2(1), 25-34
• Ayoola P.B. and A. Adeyeye. “Phytochemical and nutrient evaluation of Carica papaya (pawpaw) leaves”, IJRRAS, 2010; 5 (3), 325-328.
• Baskaran C., V. Ratha bai, S.Velu, Kubendiran Kumaran. “The efficacy of Carica Papaya leaf extract on some bacterial and a fungal strain by well diffusion method”, Asian Pacific Journal of Tropical Disease, 2012;2 (2), S658-S662
• Okwu, D.E. and M.E. Okwu. Chemical Composition of Spondia Momb in plants J. Sustain. Agri. Environ., 2004;6: 140-147
• Roman-Ramos, R., Flores-Saenz, J.L., and Alarcon-Aguilar, F.J. Anti-hyperglycemic effect of some edible plants. Journal of Ethnopharmacology.1995; 48, 25- 32.)
• Abirami N, Natarajan B, Sagadevan E.Phytochemical Investigation and In Vitro Evaluation of Hypoglycemic potential of Grewia Hirsuta. Inter. J. Pharm. Bio. Sci.; 2014;5(1): 76 – 83
• Clifford JB and Caroline D. Review article: Traditional Plant Medicines as Treatments for Diabetes, Diabetes care; 1989; 12(8).
• Megha G, Chaudhari Bhoomi B, Joshi Kinnari N, Mistry Brijesh Dabhi, Sanjay lal. In Vitro Antimicrobial Activity of Stem Bark of Bauhinia Purpurea. J Int Sci Press (India) 2013; 4:29-35.