Preparation and Cytotoxicity of Coriandrum Sativum L. Oil Loaded Chitosan Nanoparticles

Yıl 2018, Cilt: 5 Sayı: 1, 179 - 190, 01.09.2017

Yasemin Budama Kilinc Rabia Cakir Koc Zeynep Kaya

https://doi.org/10.18596/jotcsa.323798

Cited By: 3

Öz

Coriandrum sativum L.oil has antibacterial,
antifungal and antimicrobial activity. This
study aims to prepare and characterize chitosan nanoparticles loaded with Coriandrum sativum L., commonly known as coriander oil, using
ionic gelation method and to determine their cytotoxicity in cell culture.
Tripolyphosphate (TPP) was used as a cross-linker in ionic gelation method. The
characterization of the chitosan nanoparticles loaded with coriander (Coriandrum sativum L.) oil was performed
with Zeta-Sizer, Fourier transform Infrared spectrophotometer (FT-IR), scanning
electron microscopy (SEM) and electron dispersive X-ray Spectroscopy (EDS). It
was shown that chitosan nanoparticles loaded with coriander oil have an average
size of 113.5 nm, zeta potential of 16 mV and a polydispersity
index of 0.378. SEM analysis showed that they have spherical
morphology and EDS showed the element composition. Furthermore,
cytotoxicity analysis on L929 fibroblast cells was performed using XTT method
and it was observed that chitosan particles loaded with coriander oil, coriander
oil and empty chitosan nanoparticles did not provoke significant toxicity on
cells. 

Anahtar Kelimeler

chitosan, nanoparticle, Coriander (Coriandrum sativum L.), cell culture, cytotoxicity

Kaynakça

• 1. Laribi B, Kouki K, M'Hamdi M, Bettaieb T. Coriander (Coriandrum sativum L.) and its bioactive constituents. Fitoterapia. 2015;103:9-26.
• 2. Diederichsen A. Coriander: Coriandrum Sativum L: Bioversity International; 1996.
• 3. Lawrence BM. Progress in essential oils. Perfumer & flavorist. 1992;17(5):131-46.
• 4. Padmaa M. Coriandrum sativum linn: a review. Pharmacol online Newsl. 2009;3:561-73.
• 5. Matasyoh J, Maiyo Z, Ngure R, Chepkorir R. Chemical composition and antimicrobial activity of the essential oil of Coriandrum sativum. Food Chemistry. 2009;113(2):526-9.
• 6. Chang M-Y, Shen Y-L. Linalool exhibits cytotoxic effects by activating antitumor immunity. Molecules. 2014;19(5):6694-706.
• 7. Asgarpanah J, Kazemivash N. Phytochemistry, pharmacology and medicinal properties of Coriandrum sativum L. African Journal of Pharmacy and Pharmacology. 2012;6(31):2340-5.
• 8. Stashenko EE, Puertas MA, Martínez JR. SPME determination of volatile aldehydes for evaluation of in-vitro antioxidant activity. Analytical and bioanalytical chemistry. 2002;373(1):70-4.
• 9. Donsì F, Annunziata M, Sessa M, Ferrari G. Nanoencapsulation of essential oils to enhance their antimicrobial activity in foods. LWT-Food Science and Technology. 2011;44(9):1908-14.
• 10. Shukla SK, Mishra AK, Arotiba OA, Mamba BB. Chitosan-based nanomaterials: A state-of-the-art review. International journal of biological macromolecules. 2013;59:46-58. 11. Rinaudo M. Chitin and chitosan: properties and applications. Progress in polymer science. 2006;31(7):603-32.
• 12. Agnihotri SA, Mallikarjuna NN, Aminabhavi TM. Recent advances on chitosan-based micro-and nanoparticles in drug delivery. Journal of controlled release. 2004;100(1):5-28.
• 13. Uhrich KE, Cannizzaro SM, Langer RS, Shakesheff KM. Polymeric systems for controlled drug release. Chemical reviews. 1999;99(11):3181-98.
• 14. Duman F, Kaya M. Crayfish chitosan for microencapsulation of coriander (Coriandrum sativum L.) essential oil. International Journal of Biological Macromolecules. 2016;92:125-33.
• 15. Dima C, Pătraşcu L, Cantaragiu A, Alexe P, Dima Ş. The kinetics of the swelling process and the release mechanisms of Coriandrum sativum L. essential oil from chitosan/alginate/inulin microcapsules. Food chemistry. 2016;195:39-48.
• 16. Calvo P, Remunan‐Lopez C, Vila‐Jato JL, Alonso M. Novel hydrophilic chitosan‐polyethylene oxide nanoparticles as protein carriers. Journal of Applied Polymer Science. 1997;63(1):125-32.
• 17 .Reed SJB, Reed SJB. Electron microprobe analysis: Cambridge University Press Cambridge; 1975.
• 18. Kumar MNR. A review of chitin and chitosan applications. Reactive and functional polymers. 2000;46(1):1-27.
• 19. López-García J, Lehocký M, Humpolíček P, Sáha P. HaCaT keratinocytes response on antimicrobial atelocollagen substrates: extent of cytotoxicity, cell viability and proliferation. Journal of functional biomaterials. 2014;5(2):43-57.
• 20. ISO. 10993-5:2009 Biological Evaluation of Medical Devices. Part 5: Tests for In Vitro Cytotoxicity. International Organization for Standardization. Geneva, Switzerland. 2009.