Phytochemical Evaluation of Morus alba Seeds and Cold Pressed Oil

Year 2019, Volume: 6 Issue: 1, 41 - 50, 15.05.2019

Zeliha Üstün Argon , Nihal İlhan Ali Gökyer Sıdıka Büyükhelvacıgil Öztürk Beril Koparal

https://doi.org/10.18596/jotcsa.470279

Cited By: 2

Abstract

This study has focused on  Morus alba,  which is grown in Turkey mostly for  fruit production. M. alba seeds’ analysis results showed that oil, protein, ash,  ash insoluble in hydrochloric acid, total carbohydrate were  21%, 21.58±0.13g, 3.99±0.13g,  0.9±0.00g, 54.76±2.42 g/100g respectively,  main minerals were calcium, phosphorus and potassium. Dominated fatty acids were linoleic acid (80.56±0.22), palmitic acid (7.96±0.06), oleic acid (7.11±0.05).  The primary volatiles were l-limonene, 2,2-dimethyldecane, hexanal. Sterol components were found as beta-sitosterol>delta-5-avenasterol>campesterol>cholestanol with 5501.49±44.26 mg/kg total sterols. Tocopherols were δ-tocopherol, γ-tocopherol, β-tocopherol, α-tocopherol, total phenolic content, the radical scavenging activity were 257.67±4.51, 18.23±0.11, 6.71±0.13, 3.23±0.06 mg/kg, 137.1±0.36 mgGAE/100goil,  19.9±0.46% respectively.  Other physicochemical properties  were free fatty acid   (1.55±0.89% oleic acid), peroxide value  (3.23±0.55 meqO₂/kg oil), p-anisidine value (1.18±0.55) and refractive index (40⁰C) (1.4687±0.00). As a result, M. alba seeds and oil  with its fatty acid, tocopherol, sterol and mineral composition can be used as nutritional supplements. 

Keywords

Morus alba, phytochemical properties, volatile oils, fatty acid composition, sterols, tocopherols

References

• 1. Ercisli S, Orhan E. Chemical composition of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry fruits. Food Chemistry. 2007;103(4):1380–4.
• 2. Liang L, Zhu M, Li F, Yang L, Wu X, Zhao W, et al. Chemical composition, nutritional value, and antioxidant activities of eight mulberry cultivars from China. Pharmacognosy Magazine [Internet]. 2012;8(31):215. Available from: http://www.phcog.com/text.asp?2012/8/31/215/99287
• 3. TUIK TSI. Other fruits , 1988-2017 [Internet]. 2018. Available from: http://www.tuik.gov.tr/PreTablo.do?alt_id=1001
• 4. Ercisli S. A short review of the fruit germplasm resources of Turkey. Genetic Resources and Crop Evolution. 2004;51(4):419–35.
• 5. Gecgel U, Velioglu SD, Velioglu HM. Investigating some physicochemical properties and fatty acid composition of native black mulberry (Morus nigra L.) seed oil. JAOCS, Journal of the American Oil Chemists’ Society. 2011;88(8):1179–87.
• 6. Baytop T. Therapy with medicinal plants in Turkey (Past and Present). Second. İstanbul: Publications of the Istanbul University; 1999.
• 7. Elmacı Y, Altug T. Flavour evaluation of three black mulberry (Morus nigra) cultivars using GC/MS, chemical and sensory data. Journal of the Science of Food and Agriculture. 2002;82(6):632–5.
• 8. Rahman M, Akther A, Yeasmin S, Rahman S, Ferdousi S, Das K, et al. Comparative Studies on the Physicochemical Characteristics, Nutritional Value and Antimicrobial Activities of Two Varieties Indigenous Mulberry (Morus alba L. and Morus nigra L.) Seeds. International Journal of Current Research in Chemistry and Pharmaceutical Sciences. 2014;1(7):44–50.
• 9. Shukla RK, Painuly D, Shukla A, Kumar V, Singh J, Porval A, et al. Physical evaluation, proximate analysis and antimicrobial activity of Morus Nigra seeds. International Journal of Pharmacy and Pharmaceutical Sciences. 2015;7(1):191–7.
• 10. Jha S, Srivastava AK. Antibacterial , Antifungal and Pesticidal Activity of Plant Morus Alba-a Novel Approach in Po .... International Journal of Agricultural Science and Research. 2013;3(1):157–62.
• 11. Katsube T, Yamasaki M, Shiwaku K, Ishijima T, Matsumoto I, Abe K, et al. Effect of flavonol glycoside in mulberry (Morus alba L.) leaf on glucose metabolism and oxidative stress in liver in diet-induced obese mice. Journal of the Science of Food and Agriculture. 2010;90(14):2386–92.
• 12. El-Shurafa MY, Ahmed HS, Abou-Naji SE. Organic and inorganic constituents of date palm pit (seed). Date Palm Journal. 1982;1(2):275–84.
• 13. Gumus ZP, Ertas H, Yasar E, Gumus O. Classification of olive oils using chromatography, principal component analysis and artificial neural network modelling. Journal of Food Measurement and Characterization [Internet]. 2018;12(2):1325–33. Available from: http://dx.doi.org/10.1007/s11694-018-9746-z
• 14. Ferhat R, Lekbir A, Ouadah H, Kahoul MA, Khlalfa L, Larouni S, et al. Effect of extraction solvent on total phenolic content, total flavonoid content, and antioxidant activities of Algerian pomace olive oil. Ifrj. 2017;24(6):2295–303.
• 15. AğŞeleci̇ D, Gümüş ZP, Yavuz M, ŞELECİ M, Bongartz R, Stahl F, et al. A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil. Turkish Journal of Chemistry. 2015;39:801–12.
• 16. U. Celenk V, Gumus ZP, Ustun Argon Z, Buyukhelvacigil M, Karasulu E. Analysis of Chemical Compositions of 15 Different Cold-Pressed Oils Produced in Turkey : A Case Study of Tocopherol and Fatty Acid Analysis. Journal of the Turkish Chemical Society. 2018;5(1):1–18.
• 17. Absar N, Yeasmin T, Raza MS, Sarkar SK, Arisaka F. Single step purification, characterization and N-terminal sequences of a mannose specific lectin from mulberry seeds. The Protein Journal. 2005;24(6):369–77.
• 18. Imran M, Khan H, Shah M, Khan R, Khan F. Chemical composition and antioxidant activity of certain Morus species. Journal of Zhejiang University SCIENCE B [Internet]. 2010;11(12):973–80. Available from: http://www.springerlink.com/index/10.1631/jzus.B1000173
• 19. Fennema OR. Food Chemistry [Internet]. 3rd ed. New York: Marcel Dekker Inc.; 1996. 632-650 p. Available from: http://linkinghub.elsevier.com/retrieve/pii/0260877488900556
• 20. Rao Y, Xiang B. Determination of total ash and acid-insoluble ash of Chinese herbal medicine Prunellae spica by near infrared spectroscopy. Journal of the Pharmaceutical Society of Japan. 2009;129(7):881–6.
• 21. Sardesai V. Introduction to Clinical Nutrition. Third. Boca Raton, FL: CRC Press Taylor & Francis Group; 2012.
• 22. Dini I, Tenore GC, Dini A. Chemical composition, nutritional value and antioxidant properties of Allium caepa L. Var. tropeana (red onion) seeds. Food Chemistry. 2008;107(2):613–21.
• 23. Ustun Argon Z, Gokyer A. Determination of Physicochemical Properties of Nigella sativa Seed Oil from Balıkesir Region , Turkey. Chemical and Process Engineering Research. 2016;41:43–6.
• 24. Rahman MM, Akhter A, Mounuddin M, Yeasmin SM, Rahman M, Rahman S, et al. Investigation Some Physicochemical Properties, Lipids, Glycerides and Fatty acid Composition of Mulberry (Morus Alba L) Seed oil of three Different Regions of Bangladesh. American Journal of Applied Chemistry [Internet]. 2014;2(3):38–41. Available from: http://www.sciencepublishinggroup.com/journal/paperinfo.aspx?journalid=227&doi=10.11648/j.ajac.20140203.11
• 25. Harris WS, Mozaffarian D, Rimm E, Kris-Etherton P, Rudel LL, Appel LJ, et al. Omega-6 fatty acids and risk for cardiovascular disease: A science advisory from the American Heart Association nutrition subcommittee of the council on nutrition, physical activity, and metabolism; council on cardiovascular nursing; and council on epidem. Circulation. 2009;119(6):902–7.
• 26. Yilmaz MA, Durmaz G. Mulberry seed oil: A rich source of δ-Tocopherol. JAOCS, Journal of the American Oil Chemists’ Society. 2015;92(4):553–9.
• 27. Oomah BD, Ladet S, Godfrey D V., Liang J, Girard B. Characteristics of raspberry (Rubus idaeus L.) seed oil. Food Chemistry. 2000;69(2):187–93.
• 28. Parry Jr. JW. Value-adding factors in cold-pressed edible seed oils and flours. University of Maryland; 2006.
• 29. Kamal-eldin A. Effect of fatty acids and tocopherols on the oxidative stability of vegetable oils. Eur J Lipid Sci Technol. 2006;58:1051–61.
• 30. Chen Q, McGillivray D, Wen J, Zhong F, Quek SY. Co-encapsulation of fish oil with phytosterol esters and limonene by milk proteins. Journal of Food Engineering [Internet]. 2013;117(4):505–12. Available from: http://dx.doi.org/10.1016/j.jfoodeng.2013.01.011
• 31. Rathore SS, Saxena SN, Singh B. Potential health benefits of major seed spices. International Journal of Seed Spices [Internet]. 2013;3(2):1–12. Available from: http://isss.ind.in/pdf/2013volume/1.pdf
• 32. Gould MN. Cancer chemoprevention and therapy by monoterpenes. Environmental Health Perspectives. 1997;105(SUPPL. 4):977–9.