Voltammetric Analysis of Cephalexin and Cefazoline in Pharmaceutical Formulation and Biological Samples

Year 2019, Volume: 6 Issue: 2, 217 - 224, 15.06.2019

Sabriye Perçin Özkorucuklu , Besnik Uka Gizem Yıldırım Baştemur

https://doi.org/10.18596/jotcsa.469028

Cited By: 7

Abstract

In this study, the electrochemical behaviors of cephalexin
and cefazoline on disposable pencil graphite electrode were investigated in phosphate
buffer at pH of 4.5 to 6.0 by differential pulse voltammetry. Sample analysis
was performed in phosphate buffer at the optimum medium determined for each
sample. Validation parameters were studied to show the correctness,
sensitivity, and consistency of the method developed for the cephalosporins. It
was concluded that disposable pencil graphite electrode could be used
effectively in the determination of cephalosporins. The limits of detection (S/N=3)
were found to be 0.115 mM and 0.303 mM, for cephalexin and cefazoline,
respectively. The analysis of these compounds in pharmaceutical formulations
and biological samples was carried out at defined optimum conditions. The
recovery values were around 100%. 

Keywords

electrochemical biosensors, pencil graphite electrode, voltammetric methods, Beta-lactam antibiotics

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