Yıl 2021, Cilt 8 , Sayı 1, Sayfalar 69 - 78 2021-02-28

Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, cytotoxicity and genotoxicity investigations

Seda CEYLAN [1] , Burcu ALATEPELİ [2]


Cryogelation has become an advantageous method to obtain macro-porous materials with well-defined, interconnected pores for tissue engineering applications. Herein, polyvinyl alcohol and chitosan polymers (PVA-CHI) were used to produce cryogel scaffolds via cryogelation. Glutaraldehyde was used as a crosslinking agent and the effect of crosslinking amount on the properties of scaffolds investigated. Glutaraldehyde amount was divided into 5, 10, and 15% total amount of polymer concentration. The optimized pore morphology was obtained as a scaffold containing 5% glutaraldehyde amount. In addition to the FTIR, SEM, swelling, and degradation analyses, mechanical tests were performed to present the characterization properties of the cryogels. Direct and indirect cytotoxicity test and genotoxicity experiments were performed with Mouse Embryonic Fibroblasts (MEF). In addition, cell morphologies on scaffolds were analyzed with SEM. The results showed that PVA-CHI based cryogels had no genotoxic and cytotoxic effects on MEF cells and have a potential for tissue engineering applications.

chitosan, PVA, Cryogel, Genotoxicity
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Birincil Dil en
Konular Polimer Bilimi
Bölüm Makaleler
Yazarlar

Orcid: 0000-0002-1088-7886
Yazar: Seda CEYLAN (Sorumlu Yazar)
Kurum: ADANA SCIENCE AND TECHNOLOGY UNIVERSITY
Ülke: Turkey


Orcid: 0000-0003-3940-2085
Yazar: Burcu ALATEPELİ
Kurum: Çukurova Üniversitesi
Ülke: Turkey


Destekleyen Kurum Scientific Research Projects Unit of Adana AlparslanTürkeş Science and Technology University
Proje Numarası (BAP-20103004)
Tarihler

Başvuru Tarihi : 12 Kasım 2020
Kabul Tarihi : 17 Kasım 2020
Yayımlanma Tarihi : 28 Şubat 2021

Vancouver Ceylan S , Alatepeli B . Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, cytotoxicity and genotoxicity investigations. Journal of the Turkish Chemical Society Section A: Chemistry. 2021; 8(1): 69-78.