Quercetin particles with lower inhibitory activity for α-glycosidase and negligible effects on blood clotting

Yıl 2021, Cilt: 8 Sayı: 2, 443 - 452, 31.05.2021

Mehtap Şahiner Selin Sagbas Suner

https://doi.org/10.18596/jotcsa.825868

Öz

Poly(quercetin) (p(QR)) particles was synthesis by using poly(ethylene glycol) diglycidyl ether (PEGGE) crosslinker in a single step via microemulsion system. The morphological, size and functional analysis of the prepared particles were carried by optic microscope, scanning electron microscope (SEM), dynamic light scattering (DLS) measurements and FT-IR spectroscopy. P(QR) particles were found to be in spherical shape with 372±9 nm size range by means of SEM images and DLS measurements. The zeta potential measurements, performed at different pH conditions and potentiometric titration of p(QR) particles were revealed that the isoelectric point and pKa values of as around pH 2.5 and 2.3, respectively. Ferric reducing antioxidant power (FRAP) was determined for QR and p(QR) particles at pH 3.6 and found as 9.4 and 0.43 µg reduced Fe(ll). The effects of QR and p(QR) particles on alfa-glycosidase enzyme activity at pH 6.9 were investigated and found that QR molecules and p(QR) particles were capable of inhibiting the α-glycosidase enzyme 89.3% and 24.7% respectively. The fluorescence spectroscopy of QR and p(QR) in fibrinogen showed that p(QR) particles do not induce clothing of blood.

Anahtar Kelimeler

Quercetin particles, alpha glucosidase inhibitor, fibrinogen binding, antioxidant

Destekleyen Kurum

Çanakale Onsekizmart Üniversitesi

Proje Numarası

COMU BAP, FBA-2018-2725

Teşekkür

This work is supported by the Scientific Research Commission of Canakkale Onsekiz Mart University (COMU BAP, FBA-2018-2725).

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