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Fabrication and Characterization of Gelatin/Chitosan Hydrogel Membranes

Yıl 2021, Cilt 8, Sayı 4, 1045 - 1056, 30.11.2021
https://doi.org/10.18596/jotcsa.942478

Öz

The gelatin/chitosan (GEL/CTS, GC) membranes have been fabricated well by a simple in-situ method. The as-prepared GC membranes were characterized by morphological surface (scanning electron microscopy, SEM), chemical (Fourier-transform infrared spectroscopy, FT-IR), crystallinity (X-ray diffraction, XRD), thermal (thermal gravimetric analysis, TGA), mechanical (tensile test), hydrophilic (water contact angle), and swelling properties to elucidate the changes in their chemical structures and morphologies. The morphological structure of the GC membranes was found to be very smooth, non-rough and homogeneous. The FT-IR and XRD studies manifest that the GC membranes have good interaction and compatibility between GEL and CTS in the hydrogel mixture. The prepared GC membranes also obtain better thermal, mechanical and swelling properties comparing to the raw CTS molecule. These results suggest that the nontoxic GC membrane can become a preferred hydrogel membrane in the field of wound dressing or tissue-engineering applications.

Kaynakça

  • 1. Vo TS, Vo TTBC, Suk JW, Kim K. Recycling performance of graphene oxide-chitosan hybrid hydrogels for removal of cationic and anionic dyes. Nano Convergence. 2020;7, 4:1–11.
  • 2. Vo TS, Hossain MM, Jeong HM, Kim K. Heavy metal removal applications using adsorptive membranes. Nano Convergence. 2020;7, 36:2–26.
  • 3. Vo TS, Vo TTBC. Preparation and Characterization of Bis-Propargyl-Succinate, and its Application in Preliminary Healing Ability of Crosslinked Polyurethane using “Azide-Alkyne” Click. Journal of Engineering Science and Technology Review. 2020;13(4):110–6.
  • 4. Vo TS, Vo TTBC, Tien TT, Singh NT. Enhancement of mechanical property of modified polyurethane with bis-butyl succinate. Journal of the Turkish Chemical Society Section A: Chemistry. 2021;8(2):519–26. DOI:
  • 5. Vo TS, Vo TTBC. A Self-Healing Material Based on Microcapsules of Poly(Urea-Formaldehyde)/Bis-Propargyl-Succinate Containing in Polyurethane Matrix. Journal of the Turkish Chemical Society Section A: Chemistry. 2021;8(3):787–802.
  • 6. Velmurugan N, Kumar GG, Han SS, Nahm KS, Lee YS. Synthesis and Characterization of Potential Fungicidal Silver Nano-Sized Particles and Chitosan Membrane Containing Silver Particles. Iranian Polymer Journal. 2009;18(5 (107)):383–92.
  • 7. Ti Feng J, Zhou J, Zhou J, Gao L, Xing Y, XuHui L. Synthesis and Characterization of Chitosan-based Schiff Base Compounds with Aromatic Substituent Groups. Iranian Polymer Journal. 2011;20(2):123–36.
  • 8. KSV Krishna R, K Madhusudana R, Kumar PN, Il-Doo C. Novel Chitosan-based pH Sensitive Micro-networks for the Controlled Release of 5-Fluorouracil. Iranian Polymer Journal. 2010;19(4):265–76.
  • 9. Gbenebor OP, Adeosun SO, Lawal GI, Jun S, Olaleye SA. Acetylation, Crystalline and Morphological Properties of Structural Polysaccharide from Shrimp Exoskeleton. Engineering Science and Technology, an International Journal. 2017;20(3):1155–65.
  • 10. Peng Z, Li Z, Shen Y. Influence of Chemical Cross-Linking on Properties of Gelatin/Chitosan Microspheres. Polymer-Plastics Technology and Engineering. 2012;51(4):381–5.
  • 11. Zamani A, Taherzadeh MJ. Effects of Partial Dehydration and Freezing Temperature on the Morphology and Water Binding Capacity of Carboxymethyl Chitosan-Based Superabsorbents. Industrial & Engineering Chemistry Research. 2010;49(17):8094–9.
  • 12. Adair A, Kaesaman A, Klinpituksa P. Superabsorbent materials derived from hydroxyethyl cellulose and bentonite: Preparation, characterization and swelling capacities. Polymer Testing. 2017;64:321–9.
  • 13. Olad A, Pourkhiyabi M, Gharekhani H, Doustdar F. Semi-IPN superabsorbent nanocomposite based on sodium alginate and montmorillonite: Reaction parameters and swelling characteristics. Carbohydrate Polymers. 2018;190:295–306.
  • 14. Rop K, Mbui D, Njomo N, Karuku GN, Michira I, Ajayi RF. Biodegradable water hyacinth cellulose-graft-poly(ammonium acrylate-co-acrylic acid) polymer hydrogel for potential agricultural application. Heliyon. 2019;5(3):e01416.
  • 15. Pal K, Banthia AK, Majumdar DK. Preparation and characterization of polyvinyl alcohol-gelatin hydrogel membranes for biomedical applications. AAPS PharmSciTech. 2007;8(1):E1–5.
  • 16. Fan L, Yang H, Yang J, Peng M, Hu J. Preparation and characterization of chitosan/gelatin/PVA hydrogel for wound dressings. Carbohydrate Polymers. 2016;146:427–34.
  • 17. Gonzalez JS, Maiolo AS, Hoppe CE, Alvarez VA. Composite Gels Based on Poly (Vinyl alcohol) for Biomedical Uses. Procedia Materials Science. 2012;1:483–90.
  • 18. Dragan ES. Design and applications of interpenetrating polymer network hydrogels. A review. Chemical Engineering Journal. 2014;243:572–90.
  • 19. Tokura S, Ueno K, Miyazaki S, Nishi N. Molecular Weight Dependent Antimicrobial Activity by Chitosan. In: New Macromolecular Architecture and Functions. Berlin, Heidelberg: Springer Berlin Heidelberg; 1996. p. 199–207.
  • 20. Ceylan S, Alatepeli B. Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, cytotoxicity and genotoxicity investigations. Journal of the Turkish Chemical Society Section A: Chemistry. 2020;8(1):69–78.
  • 21. Dwivedi A, Bharti P, Shukla S. Surface assimilation and corrosion inhibition characteristic of water soluble Polyvinyl Alcohol on mild steel surface in 0.5M HCl solution. Journal of the Turkish Chemical Society Section A: Chemistry. 2021;8(1):217–28.
  • 22. Okuyama K, Noguchi K, Miyazawa T, Yui T, Ogawa K. Molecular and Crystal Structure of Hydrated Chitosan. Macromolecules. 1997;30(19):5849–55.
  • 23. Jayakumar R, Prabaharan M, Reis RL, Mano JF. Graft copolymerized chitosan—present status and applications. Carbohydrate Polymers. 2005;62(2):142–58.
  • 24. Jayakumar R, Reis RL, Mano JF. Chemistry and Applications of Phosphorylated Chitin and Chitosan. E-Polymers. 2006;6(1):1–16.
  • 25. Jayakumar R, Reis RL, Mano JF. Synthesis and Characterization of N ‐methylenephenyl Phosphonic Chitosan. Journal of Macromolecular Science, Part A. 2007;44(3):271–5.
  • 26. Kołodziejska I, Piotrowska B, Bulge M, Tylingo R. Effect of transglutaminase and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide on the solubility of fish gelatin–chitosan films. Carbohydrate Polymers. 2006;65(4):404–9.
  • 27. Sendemir-Urkmez A, Jamison RD. The addition of biphasic calcium phosphate to porous chitosan scaffolds enhances bone tissue developmentin vitro. Journal of Biomedical Materials Research Part A. 2007;81A(3):624–33.
  • 28. Arvanitoyannis IS, Nakayama A, Aiba S. Chitosan and gelatin based edible films: state diagrams, mechanical and permeation properties. Carbohydrate Polymers. 1998;37(4):371–82.
  • 29. Achet D, He XW. Determination of the renaturation level in gelatin films. Polymer. 1995;36(4):787–91.
  • 30. Fan M, Hu Q, Shen K. Preparation and structure of chitosan soluble in wide pH range. Carbohydrate Polymers. 2009;78(1):66–71.
  • 31. Lu S, Song X, Cao D, Chen Y, Yao K. Preparation of water-soluble chitosan. Journal of Applied Polymer Science. 2004;91(6):3497–503.
  • 32. Qin C, Li H, XIAO Q, Liu Y, Zhu J, Du Y. Water-solubility of chitosan and its antimicrobial activity. Carbohydrate Polymers. 2006;63(3):367–74.
  • 33. Borzacchiello A, Ambrosio L, Netti PA, Nicolais L, Peniche C, Gallardo A, et al. Chitosan-based hydrogels: Synthesis and characterization. Journal of Materials Science: Materials in Medicine. 2001;12(10):861–4.
  • 34. Guan YL, Shao L, De Yao K. A study on correlation between water state and swelling kinetics of chitosan-based hydrogels. Journal of Applied Polymer Science. 1996;61(13):2325–35.
  • 35. Xiao C, Lu Y, Gao S, Zhang L. Characterization of konjac glucomannan-gelatin blend films. Journal of Applied Polymer Science. 2001;79(9):1596–602.
  • 36. Cheng M, Deng J, Yang F, Gong Y, Zhao N, Zhang X. Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions. Biomaterials. 2003;24(17):2871–80.
  • 37. Zhai M, Zhao L, Yoshii F, Kume T. Study on antibacterial starch/chitosan blend film formed under the action of irradiation. Carbohydrate Polymers. 2004;57(1):83–8.
  • 38. Delmar K, Bianco-Peled H. The dramatic effect of small pH changes on the properties of chitosan hydrogels crosslinked with genipin. Carbohydrate Polymers. 2015;127:28–37.
  • 39. Tomić SL, Mićić MM, Dobić SN, Filipović JM, Suljovrujić EH. Smart poly(2-hydroxyethyl methacrylate/itaconic acid) hydrogels for biomedical application. Radiation Physics and Chemistry. 2010;79(5):643–9.

Yıl 2021, Cilt 8, Sayı 4, 1045 - 1056, 30.11.2021
https://doi.org/10.18596/jotcsa.942478

Öz

Kaynakça

  • 1. Vo TS, Vo TTBC, Suk JW, Kim K. Recycling performance of graphene oxide-chitosan hybrid hydrogels for removal of cationic and anionic dyes. Nano Convergence. 2020;7, 4:1–11.
  • 2. Vo TS, Hossain MM, Jeong HM, Kim K. Heavy metal removal applications using adsorptive membranes. Nano Convergence. 2020;7, 36:2–26.
  • 3. Vo TS, Vo TTBC. Preparation and Characterization of Bis-Propargyl-Succinate, and its Application in Preliminary Healing Ability of Crosslinked Polyurethane using “Azide-Alkyne” Click. Journal of Engineering Science and Technology Review. 2020;13(4):110–6.
  • 4. Vo TS, Vo TTBC, Tien TT, Singh NT. Enhancement of mechanical property of modified polyurethane with bis-butyl succinate. Journal of the Turkish Chemical Society Section A: Chemistry. 2021;8(2):519–26. DOI:
  • 5. Vo TS, Vo TTBC. A Self-Healing Material Based on Microcapsules of Poly(Urea-Formaldehyde)/Bis-Propargyl-Succinate Containing in Polyurethane Matrix. Journal of the Turkish Chemical Society Section A: Chemistry. 2021;8(3):787–802.
  • 6. Velmurugan N, Kumar GG, Han SS, Nahm KS, Lee YS. Synthesis and Characterization of Potential Fungicidal Silver Nano-Sized Particles and Chitosan Membrane Containing Silver Particles. Iranian Polymer Journal. 2009;18(5 (107)):383–92.
  • 7. Ti Feng J, Zhou J, Zhou J, Gao L, Xing Y, XuHui L. Synthesis and Characterization of Chitosan-based Schiff Base Compounds with Aromatic Substituent Groups. Iranian Polymer Journal. 2011;20(2):123–36.
  • 8. KSV Krishna R, K Madhusudana R, Kumar PN, Il-Doo C. Novel Chitosan-based pH Sensitive Micro-networks for the Controlled Release of 5-Fluorouracil. Iranian Polymer Journal. 2010;19(4):265–76.
  • 9. Gbenebor OP, Adeosun SO, Lawal GI, Jun S, Olaleye SA. Acetylation, Crystalline and Morphological Properties of Structural Polysaccharide from Shrimp Exoskeleton. Engineering Science and Technology, an International Journal. 2017;20(3):1155–65.
  • 10. Peng Z, Li Z, Shen Y. Influence of Chemical Cross-Linking on Properties of Gelatin/Chitosan Microspheres. Polymer-Plastics Technology and Engineering. 2012;51(4):381–5.
  • 11. Zamani A, Taherzadeh MJ. Effects of Partial Dehydration and Freezing Temperature on the Morphology and Water Binding Capacity of Carboxymethyl Chitosan-Based Superabsorbents. Industrial & Engineering Chemistry Research. 2010;49(17):8094–9.
  • 12. Adair A, Kaesaman A, Klinpituksa P. Superabsorbent materials derived from hydroxyethyl cellulose and bentonite: Preparation, characterization and swelling capacities. Polymer Testing. 2017;64:321–9.
  • 13. Olad A, Pourkhiyabi M, Gharekhani H, Doustdar F. Semi-IPN superabsorbent nanocomposite based on sodium alginate and montmorillonite: Reaction parameters and swelling characteristics. Carbohydrate Polymers. 2018;190:295–306.
  • 14. Rop K, Mbui D, Njomo N, Karuku GN, Michira I, Ajayi RF. Biodegradable water hyacinth cellulose-graft-poly(ammonium acrylate-co-acrylic acid) polymer hydrogel for potential agricultural application. Heliyon. 2019;5(3):e01416.
  • 15. Pal K, Banthia AK, Majumdar DK. Preparation and characterization of polyvinyl alcohol-gelatin hydrogel membranes for biomedical applications. AAPS PharmSciTech. 2007;8(1):E1–5.
  • 16. Fan L, Yang H, Yang J, Peng M, Hu J. Preparation and characterization of chitosan/gelatin/PVA hydrogel for wound dressings. Carbohydrate Polymers. 2016;146:427–34.
  • 17. Gonzalez JS, Maiolo AS, Hoppe CE, Alvarez VA. Composite Gels Based on Poly (Vinyl alcohol) for Biomedical Uses. Procedia Materials Science. 2012;1:483–90.
  • 18. Dragan ES. Design and applications of interpenetrating polymer network hydrogels. A review. Chemical Engineering Journal. 2014;243:572–90.
  • 19. Tokura S, Ueno K, Miyazaki S, Nishi N. Molecular Weight Dependent Antimicrobial Activity by Chitosan. In: New Macromolecular Architecture and Functions. Berlin, Heidelberg: Springer Berlin Heidelberg; 1996. p. 199–207.
  • 20. Ceylan S, Alatepeli B. Evaluation of PVA/Chitosan Cryogels as Potential Tissue Engineering Scaffolds; Synthesis, cytotoxicity and genotoxicity investigations. Journal of the Turkish Chemical Society Section A: Chemistry. 2020;8(1):69–78.
  • 21. Dwivedi A, Bharti P, Shukla S. Surface assimilation and corrosion inhibition characteristic of water soluble Polyvinyl Alcohol on mild steel surface in 0.5M HCl solution. Journal of the Turkish Chemical Society Section A: Chemistry. 2021;8(1):217–28.
  • 22. Okuyama K, Noguchi K, Miyazawa T, Yui T, Ogawa K. Molecular and Crystal Structure of Hydrated Chitosan. Macromolecules. 1997;30(19):5849–55.
  • 23. Jayakumar R, Prabaharan M, Reis RL, Mano JF. Graft copolymerized chitosan—present status and applications. Carbohydrate Polymers. 2005;62(2):142–58.
  • 24. Jayakumar R, Reis RL, Mano JF. Chemistry and Applications of Phosphorylated Chitin and Chitosan. E-Polymers. 2006;6(1):1–16.
  • 25. Jayakumar R, Reis RL, Mano JF. Synthesis and Characterization of N ‐methylenephenyl Phosphonic Chitosan. Journal of Macromolecular Science, Part A. 2007;44(3):271–5.
  • 26. Kołodziejska I, Piotrowska B, Bulge M, Tylingo R. Effect of transglutaminase and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide on the solubility of fish gelatin–chitosan films. Carbohydrate Polymers. 2006;65(4):404–9.
  • 27. Sendemir-Urkmez A, Jamison RD. The addition of biphasic calcium phosphate to porous chitosan scaffolds enhances bone tissue developmentin vitro. Journal of Biomedical Materials Research Part A. 2007;81A(3):624–33.
  • 28. Arvanitoyannis IS, Nakayama A, Aiba S. Chitosan and gelatin based edible films: state diagrams, mechanical and permeation properties. Carbohydrate Polymers. 1998;37(4):371–82.
  • 29. Achet D, He XW. Determination of the renaturation level in gelatin films. Polymer. 1995;36(4):787–91.
  • 30. Fan M, Hu Q, Shen K. Preparation and structure of chitosan soluble in wide pH range. Carbohydrate Polymers. 2009;78(1):66–71.
  • 31. Lu S, Song X, Cao D, Chen Y, Yao K. Preparation of water-soluble chitosan. Journal of Applied Polymer Science. 2004;91(6):3497–503.
  • 32. Qin C, Li H, XIAO Q, Liu Y, Zhu J, Du Y. Water-solubility of chitosan and its antimicrobial activity. Carbohydrate Polymers. 2006;63(3):367–74.
  • 33. Borzacchiello A, Ambrosio L, Netti PA, Nicolais L, Peniche C, Gallardo A, et al. Chitosan-based hydrogels: Synthesis and characterization. Journal of Materials Science: Materials in Medicine. 2001;12(10):861–4.
  • 34. Guan YL, Shao L, De Yao K. A study on correlation between water state and swelling kinetics of chitosan-based hydrogels. Journal of Applied Polymer Science. 1996;61(13):2325–35.
  • 35. Xiao C, Lu Y, Gao S, Zhang L. Characterization of konjac glucomannan-gelatin blend films. Journal of Applied Polymer Science. 2001;79(9):1596–602.
  • 36. Cheng M, Deng J, Yang F, Gong Y, Zhao N, Zhang X. Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions. Biomaterials. 2003;24(17):2871–80.
  • 37. Zhai M, Zhao L, Yoshii F, Kume T. Study on antibacterial starch/chitosan blend film formed under the action of irradiation. Carbohydrate Polymers. 2004;57(1):83–8.
  • 38. Delmar K, Bianco-Peled H. The dramatic effect of small pH changes on the properties of chitosan hydrogels crosslinked with genipin. Carbohydrate Polymers. 2015;127:28–37.
  • 39. Tomić SL, Mićić MM, Dobić SN, Filipović JM, Suljovrujić EH. Smart poly(2-hydroxyethyl methacrylate/itaconic acid) hydrogels for biomedical application. Radiation Physics and Chemistry. 2010;79(5):643–9.

Ayrıntılar

Birincil Dil İngilizce
Konular Polimer Bilimi
Bölüm Makaleler
Yazarlar

Thi Sinh VO (Sorumlu Yazar)
School of Mechanical Engineering, Sungkyunkwan University
0000-0003-3830-0474
South Korea


Tran Thi Bich Chau VO
Can Tho University
0000-0002-3049-2080
Vietnam


Truong Sinh NGUYEN Bu kişi benim
Sungkyunkwan University
South Korea


Tran Trung TİEN Bu kişi benim
Sungkyunkwan University
South Korea

Destekleyen Kurum No
Proje Numarası No
Yayımlanma Tarihi 30 Kasım 2021
Başvuru Tarihi 25 Mayıs 2021
Kabul Tarihi 20 Eylül 2021
Yayınlandığı Sayı Yıl 2021, Cilt 8, Sayı 4

Kaynak Göster

Vancouver Vo T. S. , Vo T. T. B. C. , Nguyen T. S. , Tien T. T. Fabrication and Characterization of Gelatin/Chitosan Hydrogel Membranes. Journal of the Turkish Chemical Society Section A: Chemistry. 2021; 8(4): 1045-1056.
J. Turk. Chem. Soc., Sect. A: Chem.