Comparison of Major Bioactive Components of Various Solvent Extracts from Algerian Bunium incrassatum Umbellules and Antioxidant Activity

Year 2023, Volume: 10 Issue: 2, 505 - 512, 31.05.2023

Fethi Toul , Amina Djendar

https://doi.org/10.18596/jotcsa.1184342

Abstract

The present study was designed to examine the chemical composition and the antioxidant activity of Bunium incrassatum extracts prepared in five different solvents with increasing polarities. RP-HPLC analysis resulted in the identification of 12 phenolic compounds, including 8 phenolic acids (caffeic acid, ellagic acid, ferulic acid, gallic acid, rosmarinic acid, sinapic acid, syringic acid, and vanillic acid) and 4 flavonoids (catechin, hesperetin, luteolin, and quercetin). Two different test systems were used to assess the antioxidant activity: DPPH and β-carotene/linoleic acid assays. Methanol extract exhibited the highest DPPH scavenging potential and even low inhibitory effect against β-carotene bleaching, whereas chloroform extract showed the highest effect against β-carotene bleaching and a weak scavenging potential. As expected, methanol extract recorded the highest content of total phenolics (26.57±0.15 mg GAE/g E), while chloroform extract has been found to be the richest in flavonoids.

Keywords

Bunium incrassatum, Umbellules, Antioxidant activity, Phenolic compounds, RP-HPLC

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