Influence of Peptide Bioregulators on Indicators of Hemostasis in Blood of Irradiated Experimental Animals at Low Altitude Conditions

Year 2023, Volume: 10 Issue: 4, 1001 - 1008, 11.11.2023

Gülbübü Kurmanbekova , Salkyn Beishenalieva , Nurjamal Omurzakova , Bermet Kıdıralıyeva

https://doi.org/10.18596/jotcsa.1141531

Abstract

In modern biology and medicine, much attention is paid to the study of the hemostasis regulation of some cell populations by substances of a peptide nature. They have the ability to regulate the functional and proliferative activity of cells and ensure communication in normal and pathological conditions. Research data showed that significant violations of the hemostatic system also occur with radiation damage to the body. One of the first radiation responses involves the coagulation cascade's activation, which leads to the breakdown of fibrinogen and the formation of fibrin clots. The potential threat of this is quite evident due to the existence of uranium mining tailings in several regions of the Kyrgyz Republic. Considering that significant disturbances of the hemostatic system occur during radiation damage to the body, and effective means are clearly not enough, the study of the effect of bioregulatory peptides in these conditions is of great importance. In the study, we carried out the total X-ray irradiation of laboratory animals on the X-ray therapeutic apparatus RUM-17. Peptide bioregulators peptide-1 and peptide-2 were administered intramuscularly to irradiated animals. Throughout the research, we used methods characterizing all links of hemostasis: vascular-platelet hemostasis, coagulation hemostasis, and fibrinolysis. The study has shown that the use of the peptide bioregulators in the background of acute radiation pathology leads to a decrease in the intensity of the chronic course of post-radiation thrombo hemorrhagic syndrome, contributing to an increase in the content of antithrombin III in the blood and modulating the versatile effects of endogenous heparin, which undoubtedly have a beneficial effect on the pathogenesis of radiation sickness.

Keywords

experimental animals, irradiation, hemostasis., low altitude, peptide bioregulators

References

• Rehfeld, J. F. and Bundgaard, J. R. (2010). Results and Problems in Cell Differentiation. Cellular Peptide Hormone Synthesis and Secretory Pathways. Berlin, Germany. Springer
• Favaloro, E. J., & Lippi, G. (Eds.). (n.d.). Hemostasis and Thrombosis: Methods and Protocols (2017th ed.). Humana; Softcover reprint of the original 1st ed. 2017 edition (August 12, 2018).
• Ivanov, V. T. (2010). Peptides as Universal Biological Regulators. In HERALD OF THE RUSSIAN ACADEMY OF SCIENCES (Vol. 80, Issue 5).
• Kennedy, A. R., Maity, A., & Sanzari, J. K. (2016). A Review of Radiation-Induced Coagulopathy and New Findings to Support Potential Prevention Strategies and Treatments. Radiation Research, 186(2), 121–140. https://doi.org/10.1667/RR14406.1
• Kurmanbekova, G., Beishenalieva, S., Rachkov, I., Chekirov, K., & Omurzakova, N. (2014). Advances in Environmental Biology Bioregulatory Basic Peptides Liberated from Gall-Bladder Inflammatory Wall of People Who are Sick with Acute Cholecystitis after Laparatomy Cholecystectomy. In AENSI Journals Adv. Environ. Biol (Vol. 8, Issue 10). http://www.aensiweb.com/aeb.html
• Rachkov, A. (1990). Trombogemorragicheksii sindrom pri ostroi krovopotere i luchevi bolezni v usloviyakh vysokogor’ya:Avtoref.diss. ...dokt.med.nauk, -M., 1990, -Р. 38.
• Rehfeld, J. F., & Bundgaard, J. R. (Eds.). (n.d.). Cellular Peptide Hormone Synthesis and Secretory Pathways. In Results and Problems in Cell Differentiation. Springer; 2010th edition (May 4, 2012).
• Shpakov, A. (2011). Peptides derived from the extracellular loop of receptors: structure, mechanisms of action and application in physiology and medicine. Ross Fiziol Zh Im I M Sechenova ., 97(5), 441–458.
• Stanton A.G. Biostatistics. Danilov A. translated from English; Buzikashvili NE, Smoilova DV. editor. McGraw-Hill, Inc – 1998. – 459 с.
• Treml, B., Wallner, B., Blank, C., Fries, D., & Schobersberger, W. (2022). The Influence of Environmental Hypoxia on Hemostasis—A Systematic Review. Frontiers in Cardiovascular Medicine, 9. https://doi.org/10.3389/fcvm.2022.813550