Characterization and Lidocaine Release Behavior of Chitosan/ Sodium Alginate/ Clinoptiolite Nanocomposite Hydrogel

Year 2024, Volume: 11 Issue: 4, 1483 - 1494, 03.12.2024

Sarkis Sözkes , Betül Taşdelen , Sevil Erdogan , Nadide Gülşah Gülenç , Aslıhan Koruyucu

Abstract

In this study, the novel nanocomposites were prepared from the natural biopolymers, chitosan (CS), sodium alginate (SA) and clinoptiolite (CL) particles, and also having glutaraldehyde as a crosslinker by cryogelation technique. CS biopolymer was produced from crayfish Astacus leptodactylus. Characterization of the prepared CS, CS-co-SA (CS/SA) and drug loaded CS/SA/ CL nanocomposite were performed by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) analyses. The anesthetic drug release behavior of the prepared nanocomposite was investigated for the model drug lidocaine (LD) using UV-Vis spectrophotometry and High Performance Liquid Chromatography (HPLC) techniques. The effect of different LD and CL content on the drug release behavior of the prepared nanocomposite were studied. LD release data was fitted to various kinetic models to study the drug release behavior. The LD release from all the prepared nanocomposite hydrogels fitted the Korsmeyer-Peppas model. The swelling and drug release properties of the new CS-based nanocomposite hydrogels were improved with the inclusion of SA and CL in the gel structure.

Keywords

Chitosan, Clinoptiolite, Drug Realese, Lidocaine, Sodium alginate

Supporting Institution

Scientific Research Office of Tekirdağ Namık Kemal University

Project Number

NKUBAP.06.GA.21.330 ( Research Office of Tekirdağ Namık Kemal University)

Thanks

The authors would thank the Scientific Research Office of Tekirdağ Namık Kemal University (NKUBAP.06.GA.21.330) for funding this work.

References

• 1. Kevadiya BD, Joshi GV, Mody HM, Bajaj HC. Biopolymer–cl hydrogel composites as drug carrier: host–guest intercalation and in vitro release study of lidocaine hydrochloride. Applied Clay Science, 2011; 52: 364-367. Available from: <DOI>.
• 2. Fonseca JM, Medeiros SF, Alves GM, Santos DM, Campana-Filho SP, Santos AM. Chitosan microparticles embedded with multi-responsive poly (N-vinylcaprolactam-co-itaconic acid-co-ethylene-glycol dimethacrylate)-based hydrogel nanoparticles as a new carrier for delivery of hydrophobic drugs. Colloids Surfaces B: Biointerfaces, 2019; 175: 73-83. Available from: <DOI>.
• 3. Wu M, Lin M, Li P, Huang X, Tian K, Li C. Local anesthehtic effects of lidocaine loaded carboxymethyl chitosan cross-linked with sodium alginate hydrogels for drug delivery system, cell adhesion, and pain management. Journal of Drug Delivery and Science, 2023; 79: 104007. Available from: <DOI>.
• 4. Phan VHG, Mathiyalagan R, Nguyen MT, Tran TT, Murugesan M, Ho TN, Huong H, Li Y, Thambi T. Ionically cross-linked alginate-chitosan core-shell hydrogel beads for oral delivery of insulin. International Journal of Biological Macromolecules, 2022; 222: 262-272. Available from: <DOI>.
• 5. Kutlusoy T, Oktay B, Apohan NK, Süleymanoğlu M, Kuruca SE. Chitosan-co-hyaluronic acid porous hydrogels and their application in tissue engineering. International Journal of Biological Macromolecules, 2017; 103: 366-378. Available from: <DOI>.
• 6. Zhao F, Yao D, Guo R, Deng L, Dong A, Zhang J. Composites for polymer hydrogels and nanoparticulate systems for biomedical and pharmaceutical applications. Nanomaterials (Basel), 2015; 5: 2054-2130. Available from: <DOI>.
• 7. Zhang J, Lin X, Liu J, Zhao J, Dong H, Deng L, Liu J, Dong A. Sequential thermo-induced self-gelation and acid-triggered self-release process of drug-conjugated nanoparticles: A strategy for sustained and controlled drug delivery to tumor. Journal of Materials Chemistry B, 2013; 1: 4667–4677. Available from:<DOI>
• 8. Serati-Nouri H, Jafari A, Roshangar L, Dadashpour M, Pilehvar-Soltanahmadi Y, Vlierberghe SV, Sarghami N, Biomedical applications of zeolite-based materials: a review, Materials Science and Engineering: C Materials for Biological Applications, 116, 111225, (2020). Available from: <DOI>.
• 9. Moradi S, Barati A, Tonelli AE, Hamedi H. Effect of clinoptilolite on structure and drug release behavior of chitosan/thyme oil γ-Cyclodextrin inclusion compound hydrogels. Journal of Applied Polymer Science, 2021; 138: e49822. Available from: <DOI>.
• 10. Dragan ES, Dinu MV. Advances in porous chitosan-based composite hydrogels: Synthesis and applications. Reactive and Functional Monomers, 2020; 146: 104372. Available from: <DOI>.
• 11. Tondar M, Parsa MJ, Yousefpour Y, Sharifi AM. Shetaboushehri SV. Feasibility of clinoptilolite application as a microporous carrier for pH-controlled Sözkes S et al. JOTCSA. 2024; 11(4): 1483-1494 RESEARCH ARTICLE 1493 oral delivery of aspirin. Acta Chimica Slovenica, 2014; 61: 688-693. Available from: <DOI>.
• 12. Barbosa GP, Debone HS, Severino P, Souto EB, Silva CF. Design and characterization of chitosan/zeolite composite films — Effect of zeolite type and zeolite dose on the film properties. Materials Science and Engineering C, 2016; 60: 246-254. Available from: <DOI>.
• 13. Taşdelen B, Kayaman-Apohan N, Güven O, Baysal BM. Swelling and diffusion studies of poly (N‐isopropylacrylamide/itaconic acid) copolymeric hydrogels in water and aqueous solutions of drugs. Journal of Applied Polymer Science, 2004; 91: 911-915. Available from: <DOI>.
• 14. Lee SB, Seo SM, Lim YM, Cho SK, Lee YM. Preparation of alginate/poly (N-isopropylacrylamide) hydrogels using gamma-ray irradiation grafting. Macromolecular Research, 2004; 12: 269-275. Available from: <DOI>.
• 15. Taşdelen B, Kayaman-Apohan N, Güven O, Baysal BM. Preparation of poly (N-isopropylacrylamide/itaconic acid) copolymeric hydrogels and their drug release behavior. International Journal of Pharmaceutics, 2004; 278: 343-351. Available from: <DOI>.
• 16. Akkaya R, Ulusoy U. Preparation and characterization of polyacrylamide/maleic acid -based composites. Hacettepe Journal of Biology and Chemistry, 2011; 39, 359-370. Available from: <DOI>.
• 17. Francis NL, Hunger PM, Donius AE, Riblett BW, Zavaliangos A, Wegst UG, Wheatley MA. An ice‐templated, linearly aligned chitosan‐alginate scaffold for neural tissue engineering. Journal of Biomedical Materials Research Part A, 2013; 101: 3493-3503. Available from: <DOI>.
• 18. Higuchi T. Mechanism of sustained-action medication. theoretical analysis of rate of release of solid drugs dispersed in solid matrices. Journal of Pharmaceutical Sciences, 1963; 52: 1145-1149. Available from: <DOI>.
• 19. Ullah K, Sohail M, Mannan A, Rashid H, Shah A. Murtaza G, Khan SA. Facile synthesis of chitosan based-(AMPS-co-AA) semi-IPNs as a potential drug carrier: enzymatic degradation, cyto-toxicity, and preliminary safety evaluation. Current Drug Delivery, 2019; 16: 242–253. Available from: <DOI>.
• 20. Taşdelen B, Çiftçi Dİ, Meriç Pagano S. Preparation and characterization of chitosan/AMPS/kaolinite composite hydrogels for adsorption of methylene blue. Polymer Bulletin, 2022; 79: 9643-9662. Available from: <DOI>.
• 21. Taşdelen B, Çifçi Dİ, Meriç Pagano S. Preparation and characterization of chitosan/hyaluronic acid/itaconic acid hydrogel composite to remove manganese in aqueous solution. Desalination and Water Treatment, 2021; 209: 204-211. Available from: <DOI>.
• 22. Taşdelen B. Synthesis, swelling, diffusion and cationic dye adsorption studies of semi‐IPN sodium alginate/poly (HEMA‐co‐MA) hydrogels. ChemistrySelect, 2023; 8, e202300707. Available from: <DOI>.
• 23. Abukhadra MR, Adlii A, Khim JS, Ajarem JS, Allam AA. Insight into the Technical Qualification of the Sonocogreen CaO/ Clinoptilolite Nanocomposite (CaO(NP)/Clino) as an Advanced Delivery System for 5-Fluorouracil: Equilibrium and Cytotoxicity. ACS Omega, 2021; 6: 31982−31992. Available from: <DOI>.
• 24. Taşdelen B, Çiftçi Dİ, Meriç Pagano S. Preparation of N-isopropylacrylamide/itaconic acid/pumice highly swollen composite hydrogels to explore their removal capacity of methylene blue. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2017; 519: 245–253. Available from: <DOI>.
• 25. Thakur G, Singh A, Singh I. Chitosan-montmorillonite polymer composites: formulation and evaluation of sustained release tablets of aceclofenac. Scientia Pharmaceutica, 2016; 84: 603–618. Available from: <DOI>.
• 26. Basak SC, Kumar KS, Ramlingam M. Design and release characteristics of sustained release tablet containing metformin HCl. Revista Brasileira de Ciências Farmacêuticas, 2008; 44: 477-483. Available from: <DOI>.
• 27. Ful Y, Kao WJ. Drug release kinetics and transport mechanisms of nondegradable and degradable polymeric delivery systems. Expert Opinion on Drug Delivery, 2010; 7: 429-444. Available from: <DOI>
• 28. Ruocco CD, Acocella MR, Guerra G. Release of cationic drugs from charcoal. Materials, 2019; 12: 683-687. Available from: <DOI>.
• 29. García-Couce J, Vernhes M, Bada N, Agüero L, Valdés O, Alvarez-Barreto J, Fuentes G, Almirall A, Cruz L J. Synthesis and evaluation of AlgNa-g-Poly (QCL-co-HEMA) hydrogels as platform for chondrocyte proliferation and controlled release of betamethasone. International Journal of Molecular Sciences, 2021; 22: 5730. Available from: <