Araştırma Makalesi
BibTex RIS Kaynak Göster

Effect of GLYMO/TEOS/MTEOS Sol-Gel Film Thickness on the Responses of Glucose Biosensor

Yıl 2017, Özel Sayı 1, 17 - 24, 20.10.2017

Öz

In the present study, the effect of the film thickness on the responses of a glucose biosensor was investigated. The modified platinum electrodes used in the study were constructed by immobilization of glucose oxidase under a film layer of the sol-gel coating solution. The sol-gel coating solutions were prepared by using (3-Glycidoxylpropyl) trimethoxysilane (GLYMO), tetraethoxysilane (TEOS), and methyltriethoxysilane (MTEOS). Electrochemical measurements were carried out amperometrically by determining hydrogen peroxide released by the enzymatic reaction between glucose and glucose oxidase. It was found that the amperometric responses of the sensor decreased with increasing the volume of coating solution dropped on the adsorbed enzyme. It was determined that the film thickness has a significantly influence on the sensor responses. It was observed that the amperometric responses decreased by 85% when the volume of the coating solution dropped on the electrode increased from 4 µL to 12 µL.

Kaynakça

  • 1. Li J, Chia L.S., Goh N.K., Tan S.N., Ge H. Mediated amperometric glucose sensor modified by the sol-gel method, Sens Actuators B,1997;40:135-141. DOI:10.1016/S0925-4005(97)80252-3
  • 2. Chaubey A, Malhotra B.D. Mediated biosensors, Biosens Bioelectron, 2002; 17:441-456. DOI:10.1016/S0956-5663(01)00313-X
  • 3. Freire R.S., Pessoa C.A., Mello L.D., Kubota L.T. Direct electron transfer:An approach for electrochemical biosensors with higher selectivity and sensitivity, J Braz Chem Soc, 2003; 14:230-243. http://jbcs.sbq.org.br/imageBank/PDF/v14n2a08.pdf
  • 4. Wang J. Electrochemical glucose biosensors, Chem Rev, 2008;108:814-825. DOI:10.1021/cr068123a
  • 5. Collinson M.M. Analytical applications of organically modified silicates, Mikrochim Acta, 1998; 129:149-165. DOI:10.1007/BF01244737
  • 6. Jin W, Brennan J.D., Properties and application of proteins encapsulated within sol-gel derived materials, Anal Chim Acta, 2002;461:1-36. DOI:10.1016/S0003-2670(02)00229-5
  • 7. Thenmozhi K, Narayanan S.S. Electrochemical sensor for H2O2 based on thionin immobilized 3-aminopropyltrimethoxy silane derived sol-gel thin film electrode, Sens Actuators B, 2007; 125:195-201. DOI:10.1016/j.snb.2007.02.006
  • 8. Tu Y.F., Di J.W., Chen X.J. Study of the nano-size silica sol-gel film as the matrix of chemically modified electrode, J Sol-Gel Sci Technol, 2005;33:187-191. DOI:10.1007/s10971-005-5613-6
  • 9. Pandey P.C., Upadhyay S, Pathak, H.C. A new glucose sensor based on encapsulated glucose oxidase within organically modified sol-gel glass, Sens Actuators B, 1999;60:83-89. DOI:10.1016/S0925-4005(99)00246-4
  • 10. Pauliukaite R, Brett C.M.A. Characterization of novel glucose oxysilane sol-gel electrochemical biosensors with copper hexacynoferrate mediator, Electrochim Acta, 2005;50:4973-4980. DOI:10.1016/j.electacta.2005.01.060
  • 11. Demirkıran N, Ekinci E. Immobilization of glucose oxidase in GLYMO/MTEOS sol-gel film for glucose biosensor application, Acta Chim Slov, 2012; 59:302-306. http://acta-arhiv.chem-soc.si/59/59-2-302.pdf
Yıl 2017, Özel Sayı 1, 17 - 24, 20.10.2017

Öz

Kaynakça

  • 1. Li J, Chia L.S., Goh N.K., Tan S.N., Ge H. Mediated amperometric glucose sensor modified by the sol-gel method, Sens Actuators B,1997;40:135-141. DOI:10.1016/S0925-4005(97)80252-3
  • 2. Chaubey A, Malhotra B.D. Mediated biosensors, Biosens Bioelectron, 2002; 17:441-456. DOI:10.1016/S0956-5663(01)00313-X
  • 3. Freire R.S., Pessoa C.A., Mello L.D., Kubota L.T. Direct electron transfer:An approach for electrochemical biosensors with higher selectivity and sensitivity, J Braz Chem Soc, 2003; 14:230-243. http://jbcs.sbq.org.br/imageBank/PDF/v14n2a08.pdf
  • 4. Wang J. Electrochemical glucose biosensors, Chem Rev, 2008;108:814-825. DOI:10.1021/cr068123a
  • 5. Collinson M.M. Analytical applications of organically modified silicates, Mikrochim Acta, 1998; 129:149-165. DOI:10.1007/BF01244737
  • 6. Jin W, Brennan J.D., Properties and application of proteins encapsulated within sol-gel derived materials, Anal Chim Acta, 2002;461:1-36. DOI:10.1016/S0003-2670(02)00229-5
  • 7. Thenmozhi K, Narayanan S.S. Electrochemical sensor for H2O2 based on thionin immobilized 3-aminopropyltrimethoxy silane derived sol-gel thin film electrode, Sens Actuators B, 2007; 125:195-201. DOI:10.1016/j.snb.2007.02.006
  • 8. Tu Y.F., Di J.W., Chen X.J. Study of the nano-size silica sol-gel film as the matrix of chemically modified electrode, J Sol-Gel Sci Technol, 2005;33:187-191. DOI:10.1007/s10971-005-5613-6
  • 9. Pandey P.C., Upadhyay S, Pathak, H.C. A new glucose sensor based on encapsulated glucose oxidase within organically modified sol-gel glass, Sens Actuators B, 1999;60:83-89. DOI:10.1016/S0925-4005(99)00246-4
  • 10. Pauliukaite R, Brett C.M.A. Characterization of novel glucose oxysilane sol-gel electrochemical biosensors with copper hexacynoferrate mediator, Electrochim Acta, 2005;50:4973-4980. DOI:10.1016/j.electacta.2005.01.060
  • 11. Demirkıran N, Ekinci E. Immobilization of glucose oxidase in GLYMO/MTEOS sol-gel film for glucose biosensor application, Acta Chim Slov, 2012; 59:302-306. http://acta-arhiv.chem-soc.si/59/59-2-302.pdf
Toplam 11 adet kaynakça vardır.

Ayrıntılar

Konular Mühendislik
Bölüm Makaleler
Yazarlar

Nizamettin Demirkıran

Ergun Ekinci Bu kişi benim

Yayımlanma Tarihi 20 Ekim 2017
Gönderilme Tarihi 20 Ekim 2017
Kabul Tarihi 19 Ekim 2017
Yayımlandığı Sayı Yıl 2017 Özel Sayı 1

Kaynak Göster

APA Demirkıran, N., & Ekinci, E. (2017). Effect of GLYMO/TEOS/MTEOS Sol-Gel Film Thickness on the Responses of Glucose Biosensor. Journal of the Turkish Chemical Society Section B: Chemical Engineering, 1(Sp. is. 1), 17-24.

Creative Commons Lisansı
This piece of scholarly information is licensed under Creative Commons Atıf-GayriTicari-AynıLisanslaPaylaş 4.0 Uluslararası Lisansı.

J. Turk. Chem. Soc., Sect. B: Chem. Eng. (JOTCSB)