In Vitro Assessment of Picralima nitida Ethanol Leaf Extract's Ability to Inhibit alpha-Amylase and alpha-Glucosidase Enzymes.

Okeke Chibuzor Okechukwu; Ikenna Nnaoma; Rich Joseph; Robinson Nwabueze Oguebie

doi:10.54565/jphcfum.1808963

In Vitro Assessment of Picralima nitida Ethanol Leaf Extract's Ability to Inhibit alpha-Amylase and alpha-Glucosidase Enzymes.

Abstract

This study investigated the in vitro α-amylase and α-glucosidase inhibitory activities of the n-hexane and chloroform fractions of Picralima nitida ethanol leaf extract, comparing them with acarbose, a standard antidiabetic drug. The assays were conducted at concentrations of 5, 10, 50, and 100 mg/mL to determine the dose-dependent inhibitory potential of each fraction. Results revealed that the n-hexane fraction exhibited a progressive, concentration-dependent inhibition of α-amylase activity, increasing from 47.88% at 5 mg/mL to 69.04% at 100 mg/mL. In contrast, the chloroform fraction displayed a non-linear pattern with inhibition ranging from 46.11% to 54.36%. Acarbose maintained consistently higher inhibition values (79.38–84.79%) across all concentrations. Similarly, α-glucosidase inhibition increased with concentration for both fractions, with the n-hexane fraction rising from 39.89% to 59.64% and the chloroform fraction showing a variable but moderate activity (56.55–64.04%). Acarbose again showed superior inhibition (78.46–82.30%) at all concentrations. The observed inhibitory effects suggest that P. nitida fractions possess bioactive constituents capable of modulating carbohydrate-hydrolysing enzymes in a dose-dependent manner. Although less potent than acarbose, the fractions demonstrate promising therapeutic potential for managing postprandial hyperglycemia.

Keywords

References

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Details

Primary Language

English

Subjects

Molecular Docking, Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Okeke Chibuzor Okechukwu
0009-0007-7519-1947
Nigeria

Ikenna Nnaoma ^*
0000-0001-9785-0631
Nigeria

Rich Joseph
0000-0001-7497-8069
Nigeria

Robinson Nwabueze Oguebie
0009-0004-7559-4752
Nigeria

Publication Date

December 23, 2025

Submission Date

October 23, 2025

Acceptance Date

December 7, 2025

Published in Issue

Year 2025 Volume: 8 Number: 2

DOI

https://doi.org/10.54565/jphcfum.1808963

IZ

https://izlik.org/JA75KX25CH

Cite

RIS / Bibtex

APA

Chibuzor Okechukwu, O., Nnaoma, I., Joseph, R., & Oguebie, R. N. (2025). In Vitro Assessment of Picralima nitida Ethanol Leaf Extract’s Ability to Inhibit alpha-Amylase and alpha-Glucosidase Enzymes. Journal of Physical Chemistry and Functional Materials, 8(2), 179-183. https://doi.org/10.54565/jphcfum.1808963

AMA

1.Chibuzor Okechukwu O, Nnaoma I, Joseph R, Oguebie RN. In Vitro Assessment of Picralima nitida Ethanol Leaf Extract’s Ability to Inhibit alpha-Amylase and alpha-Glucosidase Enzymes. Journal of Physical Chemistry and Functional Materials. 2025;8(2):179-183. doi:10.54565/jphcfum.1808963

Chicago

Chibuzor Okechukwu, Okeke, Ikenna Nnaoma, Rich Joseph, and Robinson Nwabueze Oguebie. 2025. “In Vitro Assessment of Picralima Nitida Ethanol Leaf Extract’s Ability to Inhibit Alpha-Amylase and Alpha-Glucosidase Enzymes”. Journal of Physical Chemistry and Functional Materials 8 (2): 179-83. https://doi.org/10.54565/jphcfum.1808963.

EndNote

Chibuzor Okechukwu O, Nnaoma I, Joseph R, Oguebie RN (December 1, 2025) In Vitro Assessment of Picralima nitida Ethanol Leaf Extract’s Ability to Inhibit alpha-Amylase and alpha-Glucosidase Enzymes. Journal of Physical Chemistry and Functional Materials 8 2 179–183.

IEEE

[1]O. Chibuzor Okechukwu, I. Nnaoma, R. Joseph, and R. N. Oguebie, “In Vitro Assessment of Picralima nitida Ethanol Leaf Extract’s Ability to Inhibit alpha-Amylase and alpha-Glucosidase Enzymes”., Journal of Physical Chemistry and Functional Materials, vol. 8, no. 2, pp. 179–183, Dec. 2025, doi: 10.54565/jphcfum.1808963.

ISNAD

Chibuzor Okechukwu, Okeke - Nnaoma, Ikenna - Joseph, Rich - Oguebie, Robinson Nwabueze. “In Vitro Assessment of Picralima Nitida Ethanol Leaf Extract’s Ability to Inhibit Alpha-Amylase and Alpha-Glucosidase Enzymes”. Journal of Physical Chemistry and Functional Materials 8/2 (December 1, 2025): 179-183. https://doi.org/10.54565/jphcfum.1808963.

JAMA

1.Chibuzor Okechukwu O, Nnaoma I, Joseph R, Oguebie RN. In Vitro Assessment of Picralima nitida Ethanol Leaf Extract’s Ability to Inhibit alpha-Amylase and alpha-Glucosidase Enzymes. Journal of Physical Chemistry and Functional Materials. 2025;8:179–183.

MLA

Chibuzor Okechukwu, Okeke, et al. “In Vitro Assessment of Picralima Nitida Ethanol Leaf Extract’s Ability to Inhibit Alpha-Amylase and Alpha-Glucosidase Enzymes”. Journal of Physical Chemistry and Functional Materials, vol. 8, no. 2, Dec. 2025, pp. 179-83, doi:10.54565/jphcfum.1808963.

Vancouver

1.Okeke Chibuzor Okechukwu, Ikenna Nnaoma, Rich Joseph, Robinson Nwabueze Oguebie. In Vitro Assessment of Picralima nitida Ethanol Leaf Extract’s Ability to Inhibit alpha-Amylase and alpha-Glucosidase Enzymes. Journal of Physical Chemistry and Functional Materials. 2025 Dec. 1;8(2):179-83. doi:10.54565/jphcfum.1808963