Revolutionizing Technique to Control Mycoplasma gallisepticum in Hatching Eggs Using Zinc oxide, Antibiotic nano-particles

Year 2025, Volume: 22 Issue: 2, 61 - 67, 28.12.2025

Hussein Kaoud

Abstract

Zinc oxide (ZnO), Flumequine, and Tiamulin nanoparticles were designed to target Mycoplasma gallisepticum (MG) without adversely affecting egg-hatching potential. 3,000 eggs were selected from 3,500 of Hy-Line breeder flock which were divided into 5 equal groups (600 eggs each) as detailed below: Group A (infected by MG and sanitized by using ZnO nanoparticles); Group Bf (infected by MG and sanitized by using Flumequine nanoparticles); Group Bt (infection by MG and sanitized by Tiamulin nanoparticles); Group C (infected by MG, serving as the positive control); Group D (uninfected and untreated, serving as the negative control). The findings revealed a statistically significant reduction (P<0.05) in colony-forming units per millilitre (CFU/mL) of MG and Total Bacterial Colony Count (TBCC) in groups: A, Bf and Bt after 5 days of treatment as compared to group C. The treatments exhibited notable
bactericidal properties while allowing for normal embryo development, hatching, and mortality rates. Eggs treated with these antibacterial agents experienced a significant decrease (P < 0.001) in embryonic mortality during incubation. ZnO NOs, Flumequine,
and Tiamulin nanoparticles exhibit promise in preventing Mycoplasma gallisepticum -induced hatchery infection.

Keywords

Flumeqine NPs , Tiamulin NPs , Vertical transmission , Mycoplasma gallisepticum , Ultra-Nano-Fog

Ethical Statement

The animal experiment was conducted in strict accordance with and adherence to the relevant policies regarding animal handling as mandated under international, national, and/or institutional guide lines for the care of animals and was approved by the Research Ethical Committee at the National Research Centre, Cairo, Egypt.

Supporting Institution

Cairo University

Thanks

The author acknowledges the financial support provided by Cairo University-Egypt.

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