Synthesis and in vitro anticancer activity of some new hydrazide-hydrazones derived from articaine

Year 2024, Volume: 28 Issue: 6, 1901 - 1910, 28.06.2025

Sevgi Karakuş , Fatih Tok , Burçin İrem Abas , Elif Tütüncü Nigar Kübra Kalkan Sevda Türk , Özge Çevik

https://doi.org/10.29228/jrp.863

https://izlik.org/JA42NU88JH

Abstract

In this study, synthesis of some new hydrazone derivatives based on articaine was carried out. MDA-MB 231 (triple negative human breast cancer cells) and HUVEC (human umbilical vein endothelial cells) cells were used to investigate the cytotoxic activity of hydrazone compounds. Induction of apoptosis and cell viability were assessed by AnnexinV-PI binding levels and Bax-Bcl2 gene expression levels. Inhibitory activities of the compounds against carbonic anhydrase enzyme were also evaluated. Compounds 2b and 2m exhibited the highest cytotoxic activity against MDA MB-231 cells with IC50 values of 16.62±1.18 µM and 18.56±2.36 µM, respectively. Similarly, the CA inhibition of 2b and 2m was also determined to be the highest in the series.

Keywords

Hydrazone , Articaine , breast cancer , carbonic anhydrase , bax

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