Evaluation of antimicrobial and antibiofilm activities of some Phytolacca americana L. extracts

Year 2026, Volume: 30 Issue: 1, 51 - 59, 11.01.2026

Gülbeyaz Dökmeci , Kevser Taban , İpek Süntar , Osman Tugay , Berrin Özçelik

https://doi.org/10.12991/jrespharm.1629289

Abstract

Antimicrobial resistance remains a significant global challenge across various fields. To solve this problem, researchers are working on developing antimicrobial agents, antiseptics, and disinfectants with different formulations. In this study, methanol (MeOH) extracts of Phytolacca americana L. fruits and leaves, were evaluated for their both antimicrobial and antibiofilm activities using microdilution method and antibiofilm assay against bacteria and fungi including Staphylococcus aureus, Streptococcus mutans, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Candida albicans, C. parapsilosis, C. krusei, Trichophyton rubrum, Epidermophyton floccosum, and Microsporum gypseum.. According to the activity studies, extracts displayed 32-128 µg/mL MICs against tested bacteria and fungi. In the microduliton method; inhibition curves of IC50 obtained by the resazurin microplate assay for the extracts showed results in the range of 12-41 μg/mL.

Keywords

Antimicrobial , antibiofilm , broth microdilution , colorimetric microdilution , Phytolacca , disinfectant , pharmaceutical agents

Supporting Institution

Gazi University

Project Number

TYL-2024-9449

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