Synthesis, antiproliferative activity and plasmid DNA interaction studies of [Pt(1-substituted-1H-1,3-diazole)2Cl2] and [Pt(1-substituted-1H-1,3-diazole)2I2] analogs

Özge Eryiğit; Elif Ergin; Nebahat Aytuna Çerçi; Leyla Açık; Semra Utku

doi:10.12991/jrespharm.1633032

Synthesis, antiproliferative activity and plasmid DNA interaction studies of [Pt(1-substituted-1H-1,3-diazole)2Cl2] and [Pt(1-substituted-1H-1,3-diazole)2I2] analogs

Abstract

Researchers have accelerated subject their research on platinum complexes since the serendipitous discovery of cisplatin's anticancer properties. Their goal is to synthesize more potent platinum complexes by replacing the carrier ammonia ligands in the structure of cisplatin with biologically active heterocyclic compounds. 1H-1,3-Diazoles are heterocyclic compounds with a five-member ring structure well-known to biological systems. Their exceptional pharmacological activities have led to a remarkable place in recent years. Their exceptional pharmacological activities have led to a remarkable place in the past few decades. This approach is a key strategy for increasing the efficacy of cancer treatments and better-targeting cancer types that develop resistance to current therapies. In this study, six novel platinum(II) complexes with the structures of [Pt(L1)2Cl2] (1a), [Pt(L2)2Cl2] (1b), [Pt(L3)2Cl2] (1c), [Pt(L1)2I2] (2a), [Pt(L2)2I2] (2b), and [Pt(L3)2I2] (2c) (L1= 1-(4-methoxyphenyl)-1H-1,3-diazole, L2= 1-phenyl-1H-1,3-diazole and L3= 1-benzyl-1H-1,3-diazole) were synthesized for cisplatin analogues. The chemical structures of 1a-1c and 2a-2c were elucidated by Fourier Transform Infrared (FT-IR), Proton Nuclear Magnetic Resonance (1H-NMR), Electrospray Ionization Mass Spectrometry (ESI-MS), and elemental analysis. The interactions of the complexes with pBR322 plasmid DNA and BamHI and HindIII restriction endonuclease enzymes were investigated by agarose gel electrophoresis. The cytotoxic effects of 1a-1c and 2a-2c against human non-small cell lung cancer (A549), human colon adenocarcinoma (CaCo-2), and mouse fibroblast cells (L929) cells were tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Compound 2c was as effective as cisplatin against the CaCo-2 cell line with 17.27 IC50 values.

Keywords

Supporting Institution

Mersin University Scientific Research Projects Coordination Unit

Project Number

2016-2-TP2-1524

References

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Details

Primary Language

English

Subjects

Pharmaceutical Chemistry

Journal Section

Research Article

Authors

Özge Eryiğit
0009-0004-0366-7801
Türkiye

Elif Ergin
0000-0001-5014-9143
Türkiye

Nebahat Aytuna Çerçi
0000-0002-7864-7213
Türkiye

Leyla Açık
0000-0002-3672-8429
Türkiye

Semra Utku ^*
0000-0003-3181-9134
Türkiye

Publication Date

January 11, 2026

Submission Date

February 5, 2025

Acceptance Date

March 14, 2025

Published in Issue

Year 2026 Volume: 30 Number: 1

DOI

https://doi.org/10.12991/jrespharm.1633032

IZ

https://izlik.org/JA49NU29KG

Cite

RIS / Bibtex

APA

Eryiğit, Ö., Ergin, E., Çerçi, N. A., Açık, L., & Utku, S. (2026). Synthesis, antiproliferative activity and plasmid DNA interaction studies of [Pt(1-substituted-1H-1,3-diazole)2Cl2] and [Pt(1-substituted-1H-1,3-diazole)2I2] analogs. Journal of Research in Pharmacy, 30(1), 28-38. https://doi.org/10.12991/jrespharm.1633032

AMA

1.Eryiğit Ö, Ergin E, Çerçi NA, Açık L, Utku S. Synthesis, antiproliferative activity and plasmid DNA interaction studies of [Pt(1-substituted-1H-1,3-diazole)2Cl2] and [Pt(1-substituted-1H-1,3-diazole)2I2] analogs. J. Res. Pharm. 2026;30(1):28-38. doi:10.12991/jrespharm.1633032

Chicago

Eryiğit, Özge, Elif Ergin, Nebahat Aytuna Çerçi, Leyla Açık, and Semra Utku. 2026. “Synthesis, Antiproliferative Activity and Plasmid DNA Interaction Studies of [Pt(1-Substituted-1H-1,3-Diazole)2Cl2] and [Pt(1-Substituted-1H-1,3-Diazole)2I2] Analogs”. Journal of Research in Pharmacy 30 (1): 28-38. https://doi.org/10.12991/jrespharm.1633032.

EndNote

Eryiğit Ö, Ergin E, Çerçi NA, Açık L, Utku S (January 1, 2026) Synthesis, antiproliferative activity and plasmid DNA interaction studies of [Pt(1-substituted-1H-1,3-diazole)2Cl2] and [Pt(1-substituted-1H-1,3-diazole)2I2] analogs. Journal of Research in Pharmacy 30 1 28–38.

IEEE

[1]Ö. Eryiğit, E. Ergin, N. A. Çerçi, L. Açık, and S. Utku, “Synthesis, antiproliferative activity and plasmid DNA interaction studies of [Pt(1-substituted-1H-1,3-diazole)2Cl2] and [Pt(1-substituted-1H-1,3-diazole)2I2] analogs”, J. Res. Pharm., vol. 30, no. 1, pp. 28–38, Jan. 2026, doi: 10.12991/jrespharm.1633032.

ISNAD

Eryiğit, Özge - Ergin, Elif - Çerçi, Nebahat Aytuna - Açık, Leyla - Utku, Semra. “Synthesis, Antiproliferative Activity and Plasmid DNA Interaction Studies of [Pt(1-Substituted-1H-1,3-Diazole)2Cl2] and [Pt(1-Substituted-1H-1,3-Diazole)2I2] Analogs”. Journal of Research in Pharmacy 30/1 (January 1, 2026): 28-38. https://doi.org/10.12991/jrespharm.1633032.

JAMA

1.Eryiğit Ö, Ergin E, Çerçi NA, Açık L, Utku S. Synthesis, antiproliferative activity and plasmid DNA interaction studies of [Pt(1-substituted-1H-1,3-diazole)2Cl2] and [Pt(1-substituted-1H-1,3-diazole)2I2] analogs. J. Res. Pharm. 2026;30:28–38.

MLA

Eryiğit, Özge, et al. “Synthesis, Antiproliferative Activity and Plasmid DNA Interaction Studies of [Pt(1-Substituted-1H-1,3-Diazole)2Cl2] and [Pt(1-Substituted-1H-1,3-Diazole)2I2] Analogs”. Journal of Research in Pharmacy, vol. 30, no. 1, Jan. 2026, pp. 28-38, doi:10.12991/jrespharm.1633032.

Vancouver

1.Özge Eryiğit, Elif Ergin, Nebahat Aytuna Çerçi, Leyla Açık, Semra Utku. Synthesis, antiproliferative activity and plasmid DNA interaction studies of [Pt(1-substituted-1H-1,3-diazole)2Cl2] and [Pt(1-substituted-1H-1,3-diazole)2I2] analogs. J. Res. Pharm. 2026 Jan. 1;30(1):28-3. doi:10.12991/jrespharm.1633032