Phytochemical screening and anticancer profiling of Artocarpus altilis (Breadfruit) leaves against colorectal cancer

Pattilthodika Suhail; Hanmant Vatre Ashwini; Kiliyamannil Asna; Kausthubham Bagyasree; Arunachalam Thanga Thirupathi; Jibin Joy

doi:10.12991/jrespharm.1658438

Phytochemical screening and anticancer profiling of Artocarpus altilis (Breadfruit) leaves against colorectal cancer

Abstract

Artocarpus altilis (A. altilis), a member of Moraceae family is a tropical tree, typically cultivated for its edible fruit. The study aimed to investigate the phytochemical composition and evaluation of in silico and in vitro anticancer activity of ethanolic extract of A. altilis leaves. LC-MS analysis was conducted to identify the presence of bioactive compounds, whereas a molecular docking study was used to find out the interaction between the phytochemicals and the DNA-metabolising enzyme topoisomerase II. MTT assay was used to assess the cytotoxicity and anticancer efficacy of the extract using HCT-116 cell lines. Through phytochemical analysis, various phytochemicals, which are known for their anticancer properties such as aminopterin, purpurin and oleuropein were identified. The molecular docking analysis revealed that the compounds, aminopterin and purpurin possess greater binding affinity with topoisomerase II, which is crucial for DNA replication and cell division in cancer cells. In MTT assay, the extract at various doses exhibited cytotoxic effects on HCT-116 cancer cell line, with an IC50 value of 153.8±0.58 µg/ml. The study reveals that the phytoconstituents of the ethanolic extract of A. altilis leaves inhibit cancer cell proliferation and their potential for use in colorectal cancer treatment.

Keywords

References

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Details

Primary Language

English

Subjects

Basic Pharmacology, Medical Pharmacology

Journal Section

Research Article

Authors

Pattilthodika Suhail ^*
0000-0002-9564-2158
India

Hanmant Vatre Ashwini This is me
0009-0004-3025-4287
India

Kiliyamannil Asna This is me
0009-0009-0409-0123
India

Kausthubham Bagyasree This is me
0009-0002-7319-9244
India

Arunachalam Thanga Thirupathi This is me
0009-0002-9786-3605
India

Jibin Joy This is me
0009-0002-5467-6623
India

Publication Date

January 11, 2026

Submission Date

March 15, 2025

Acceptance Date

April 29, 2025

Published in Issue

Year 2026 Volume: 30 Number: 1

DOI

https://doi.org/10.12991/jrespharm.1658438

IZ

https://izlik.org/JA46CE78MW

Cite

RIS / Bibtex

APA

Suhail, P., Ashwini, H. V., Asna, K., Bagyasree, K., Thanga Thirupathi, A., & Joy, J. (2026). Phytochemical screening and anticancer profiling of Artocarpus altilis (Breadfruit) leaves against colorectal cancer. Journal of Research in Pharmacy, 30(1), 94-105. https://doi.org/10.12991/jrespharm.1658438

AMA

1.Suhail P, Ashwini HV, Asna K, Bagyasree K, Thanga Thirupathi A, Joy J. Phytochemical screening and anticancer profiling of Artocarpus altilis (Breadfruit) leaves against colorectal cancer. J. Res. Pharm. 2026;30(1):94-105. doi:10.12991/jrespharm.1658438

Chicago

Suhail, Pattilthodika, Hanmant Vatre Ashwini, Kiliyamannil Asna, Kausthubham Bagyasree, Arunachalam Thanga Thirupathi, and Jibin Joy. 2026. “Phytochemical Screening and Anticancer Profiling of Artocarpus Altilis (Breadfruit) Leaves Against Colorectal Cancer”. Journal of Research in Pharmacy 30 (1): 94-105. https://doi.org/10.12991/jrespharm.1658438.

EndNote

Suhail P, Ashwini HV, Asna K, Bagyasree K, Thanga Thirupathi A, Joy J (January 1, 2026) Phytochemical screening and anticancer profiling of Artocarpus altilis (Breadfruit) leaves against colorectal cancer. Journal of Research in Pharmacy 30 1 94–105.

IEEE

[1]P. Suhail, H. V. Ashwini, K. Asna, K. Bagyasree, A. Thanga Thirupathi, and J. Joy, “Phytochemical screening and anticancer profiling of Artocarpus altilis (Breadfruit) leaves against colorectal cancer”, J. Res. Pharm., vol. 30, no. 1, pp. 94–105, Jan. 2026, doi: 10.12991/jrespharm.1658438.

ISNAD

Suhail, Pattilthodika - Ashwini, Hanmant Vatre - Asna, Kiliyamannil - Bagyasree, Kausthubham - Thanga Thirupathi, Arunachalam - Joy, Jibin. “Phytochemical Screening and Anticancer Profiling of Artocarpus Altilis (Breadfruit) Leaves Against Colorectal Cancer”. Journal of Research in Pharmacy 30/1 (January 1, 2026): 94-105. https://doi.org/10.12991/jrespharm.1658438.

JAMA

1.Suhail P, Ashwini HV, Asna K, Bagyasree K, Thanga Thirupathi A, Joy J. Phytochemical screening and anticancer profiling of Artocarpus altilis (Breadfruit) leaves against colorectal cancer. J. Res. Pharm. 2026;30:94–105.

MLA

Suhail, Pattilthodika, et al. “Phytochemical Screening and Anticancer Profiling of Artocarpus Altilis (Breadfruit) Leaves Against Colorectal Cancer”. Journal of Research in Pharmacy, vol. 30, no. 1, Jan. 2026, pp. 94-105, doi:10.12991/jrespharm.1658438.

Vancouver

1.Pattilthodika Suhail, Hanmant Vatre Ashwini, Kiliyamannil Asna, Kausthubham Bagyasree, Arunachalam Thanga Thirupathi, Jibin Joy. Phytochemical screening and anticancer profiling of Artocarpus altilis (Breadfruit) leaves against colorectal cancer. J. Res. Pharm. 2026 Jan. 1;30(1):94-105. doi:10.12991/jrespharm.1658438