Determination of protonation constant value of thiabendazole in ethanol-water binary mixtures by green RPLC method

Sema Berfin Kaplan; İlkay Konçe; Ebru Çubuk Demiralay

Determination of protonation constant value of thiabendazole in ethanol-water binary mixtures by green RPLC method

Abstract

In this study, an environmentally friendly analysis of an anti-infective/anthelmintic drug thiabendazole was carried out by liquid chromatography method using ethanol solvent that is not harmful to human health and the environment. Protonation constant ( 𝑝𝐾𝑎 𝑠 ) values of thiabendazole in 15, 20, 25, and 30% (v/v) ethanol-water binary 𝑠 mixtures at 37 oC were obtained by using the green reverse phase liquid chromatography method. The method is based on a model that relates the chromatographic behavior of the determined analytes to pH. The linear relationship between the solvatochromic parameter 𝐸𝑇 𝑁 and the retention values of the compound was determined depending on the variation of the organic solvent in the mobile phase. The relationship between the calculated 𝑠 value and the macroscopic 𝑠 𝑝𝐾𝑎 properties of ethanol was investigated. Compatibility was observed between the pKa ( 𝑝𝐾𝑎 𝑤 ) values in the aqueous 𝑤 medium determined by this linear approach. By using the 𝑤 value calculated for thiabendazole, the ionization 𝑤 𝑝𝐾𝑎 percentages were calculated at different pH (1-12) values with the Henderson-Hasselbalch equation.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Sema Berfin Kaplan This is me
0009-0004-1872-0484
Türkiye

İlkay Konçe
0000-0003-3542-7090
Türkiye

Ebru Çubuk Demiralay ^*
0000-0002-6270-7509
Türkiye

Publication Date

June 28, 2025

Submission Date

April 27, 2023

Acceptance Date

June 9, 2023

Published in Issue

Year 2024 Volume: 28 Number: 1

IZ

https://izlik.org/JA45RW38SZ

Cite

RIS / Bibtex

APA

Kaplan, S. B., Konçe, İ., & Çubuk Demiralay, E. (2025). Determination of protonation constant value of thiabendazole in ethanol-water binary mixtures by green RPLC method. Journal of Research in Pharmacy, 28(1), 40-50. https://izlik.org/JA45RW38SZ

AMA

1.Kaplan SB, Konçe İ, Çubuk Demiralay E. Determination of protonation constant value of thiabendazole in ethanol-water binary mixtures by green RPLC method. J. Res. Pharm. 2025;28(1):40-50. https://izlik.org/JA45RW38SZ

Chicago

Kaplan, Sema Berfin, İlkay Konçe, and Ebru Çubuk Demiralay. 2025. “Determination of Protonation Constant Value of Thiabendazole in Ethanol-Water Binary Mixtures by Green RPLC Method”. Journal of Research in Pharmacy 28 (1): 40-50. https://izlik.org/JA45RW38SZ.

EndNote

Kaplan SB, Konçe İ, Çubuk Demiralay E (June 1, 2025) Determination of protonation constant value of thiabendazole in ethanol-water binary mixtures by green RPLC method. Journal of Research in Pharmacy 28 1 40–50.

IEEE

[1]S. B. Kaplan, İ. Konçe, and E. Çubuk Demiralay, “Determination of protonation constant value of thiabendazole in ethanol-water binary mixtures by green RPLC method”, J. Res. Pharm., vol. 28, no. 1, pp. 40–50, June 2025, [Online]. Available: https://izlik.org/JA45RW38SZ

ISNAD

Kaplan, Sema Berfin - Konçe, İlkay - Çubuk Demiralay, Ebru. “Determination of Protonation Constant Value of Thiabendazole in Ethanol-Water Binary Mixtures by Green RPLC Method”. Journal of Research in Pharmacy 28/1 (June 1, 2025): 40-50. https://izlik.org/JA45RW38SZ.

JAMA

1.Kaplan SB, Konçe İ, Çubuk Demiralay E. Determination of protonation constant value of thiabendazole in ethanol-water binary mixtures by green RPLC method. J. Res. Pharm. 2025;28:40–50.

MLA

Kaplan, Sema Berfin, et al. “Determination of Protonation Constant Value of Thiabendazole in Ethanol-Water Binary Mixtures by Green RPLC Method”. Journal of Research in Pharmacy, vol. 28, no. 1, June 2025, pp. 40-50, https://izlik.org/JA45RW38SZ.

Vancouver

1.Sema Berfin Kaplan, İlkay Konçe, Ebru Çubuk Demiralay. Determination of protonation constant value of thiabendazole in ethanol-water binary mixtures by green RPLC method. J. Res. Pharm. [Internet]. 2025 Jun. 1;28(1):40-5. Available from: https://izlik.org/JA45RW38SZ