Preparation and in vitro evaluation of Tacrolimus loaded liposomal vesicles by two methods: A comparative study

Abstract

Psoriasis is an autoimmune disorder characterized by hyper proliferation of the epidermal layer at the typical body sites, including nails, scalps, cleft and knees. Liposomes are the best carrier for effective delivery of bioactives in the deep skin areas. In the present study, a comparative study of liposomes was carried out using thin film hydration and curvature tuned methods. Liposomes were prepared using cholesterol, soy lecithin and Span 80. The liposomes were evaluated for physical characterization, morphology, surface charge and release properties. Liposomes prepared by the thin film hydration method (formulation TACTL2) had better properties compared to liposomes prepared by the curvature tuned method (formulation TACCT2) in terms of entrapment efficiency (78.5±1.8% vs 53.4±5.6%), particle size (411.8 nm vs 630 nm), surface morphology(round and dispersed particles vs aggregates and lumps), and release profile (70.1% vs 32.87% in 12 h). Based on the results it is concluded that the thin film hydration method is better than a curvature tuned method for the preparation of liposomes.

Keywords

• Curvature tuned method
• film hydration method
• liposomes
• particle size
• psoriasis

References

1. [1] Ramanunny AK, Wadhwa S, Singh SK, Sharma DS, Khursheed R, Awasthi A. Treatment strategies against psoriasis: principle, perspectives and practices. Curr Drug Deliv. 2020; 17(1):52-73. [CrossRef]
2. [2] Jindal S, Awasthi R, Singhare D, Kulkarni GT. Topical delivery of Tacrolimus using liposome containing gel: An emerging and synergistic approach in management of psoriasis. Med Hypotheses. 2020; 142: 109838. [CrossRef]
3. [3] Deng Y, Chang C, Lu Q. Theinflammatoryresponse in psoriasis: a comprehensivereview. Clin Rev Allergy Immunol. 2016; 50(3):377-389. [CrossRef]
4. [4] Zeng J, Luo S, Huang Y, Lu Q. Critical role of environmental factors in the pathogenesis of psoriasis. J Dermatol. 2017; 44(8): 863-872. [CrossRef]
5. [5] Takeshita J, Grewal S, Langan SM, Mehta NN, Ogdie A, Van Voorhees AS, Gelfand JM. Psoriasis and comorbiddiseases part I. Epidemiology. J Am Acad Dermatol. 2017: 76(3); 377-390. [CrossRef]
6. [6] Badri W, Eddabra R, Fessi H, Elaissari A. Biodegradable polymer based nanoparticles: Dermal and transdermal drug delivery. J Colloid SciBiotechnol.2014; 3(2): 141–149. [CrossRef]
7. [7] Ramos Campos EV, Proença PL, Doretto-Silva L, Andrade-Oliveira V, Fraceto LF, de Araujo DR. Trends in nanoformulations for atopic dermatitis treatment. Expert Opin Drug Deliv. 2020. [CrossRef]
8. [8] Ingebrigtsen SG, Škalko-Basnet N, Holsæter AM. Development and optimization of a new processing approach for manufacturing topical liposomes-in-hydrogel drug formulations by dual asymmetric centrifugation. Drug Dev Ind Pharm. 2016; 42(9): 1375-1383. [CrossRef]

9. [9] Sala M, Diab R, Elaissari A, Fessi H. Lipid nanocarriers as skin drug delivery systems: Properties, mechanisms of skin interactions and medical applications. Int J Pharm. 2018; 535(1-2): 1-7. [CrossRef]
10. [10] Jada A.A special issue on inorganic colloidal particles, synthesis, surface properties and applications. J Colloid SciBiotechnol.2014; 3: 1-2. [CrossRef]
11. [11] Tiwari G, Tiwari R, Sriwastawa B, Bhati L, Pandey S, Pandey P, Bannerjee SK. Drug delivery systems: An updated review.Int J Pharm Investig. 2012; 2(1): 2–11. [CrossRef]
12. [12] Sherry M, Charcosset C, Fessi H, Greige-Gerges H. Essential oils encapsulated in liposomes: A review. J Liposome Res. 2013; 23(4): 268–275. [CrossRef]
13. [13] Sercombe L, Veerati T, Moheimani F, Wu SY, Sood AK, Hua S. Advances and challenges of liposome assisted drug delivery. Front Pharmacol. 2015; 1(6):286. [CrossRef]
14. [14] Bangham AD, Standish MM, Watkins JC. Diffusion of univalent ions across the lamellae of swollen phospholipids. J Mol Biol. 1965; 13(1):238–252. [CrossRef]
15. [15] Woodbury DJ, Richardson ES, Grigg AW, Welling RD, Knudson BH. Reducing liposome size with ultrasound: Bimodal size distributions. J Liposome Res. 2006; 16(1): 57–80. [CrossRef]
16. [16] Ong SGG, Chitneni M, Lee KS, Ming LC, Yuen KH. Evaluation of extrusion technique for nanosizing liposomes. Pharmaceutics. 2016; 8(4): 36. [CrossRef]
17. [17] Patil YP, Jadhav S. Novel methods for liposome preparation. Chem Phys Lipids. 2014; 177: 8–18. [CrossRef]
18. [18] Genc R, Ortiz M, O Sullivan CK. Curvature-tuned preparation of nanoliposomes. Langmuir. 2009; 25(21): 12604-12613. [CrossRef]
19. [19] Sharma A, Sharma US. Liposomes in drug delivery: progress and limitations. Int J Pharm. 1997; 154(2): 123-140.
20. [20] Awasthi R, Kumar S, Kulkarni GT. Frontier lipid-based carrier systems for drug targeting: A laconic review on niosomes. Pharm Nanotechnol. 2014; 2(3): 116-128.
21. [21] Varona S, Martin A, Cocero MJ. Liposomal incorporation of lavandin essential oil by a thin-film hydration method and by particles from gas-saturated solutions. IndEngChem Res. 2011; 50(4): 2088-2097. [CrossRef]
22. [22] Verma DD, Verma S, Blume G, Fahr A. Particle size of liposomes influences dermal delivery of substances into skin. Int J Pharm. 2003; 258(1-2):141-151. [CrossRef]
23. [23] Ola H, Yahiya SA, El-Gazayerly ON. Effect of formulation design and freeze-drying on properties of fluconazole multilamellar liposomes. Saudi Pharm J. 2010; 18(4): 217–224. [CrossRef]
24. [24] Fang JY, Hong CT, Chiu WT, Wang YY. Effect of liposomes and niosomes on skin permeation of enoxacin. Int J Pharm. 2019; 219(1-2):61-72. [CrossRef]
25. [25] Dragicevic-Curic N, Winter S, Stupar M, Milic J, Krajišnik D, Gitter B, Fahr A. Temoporfin-loaded liposomal gels: Viscoelastic properties and in vitro skin penetration. Int J Pharm. 2009; 373(1-2): 77–84. [CrossRef]
26. [26] Lasic DD. The mechanism of vesicle formation. Biochem J. 1988; 256(1):1-11. [CrossRef]
27. [27] Laouini A, Jaafar-Maalej C, Limayem-Blouza I, Sfar S, Charcosset C, Fessi H. Preparation, characterization and applications of liposomes: State of the art. J Colloid Sci. 2012: 1(2): 147-168. [CrossRef]
28. [28] Katiyar SS, Muntimadugu E, Rafeeqi TA, Domb AJ, Khan W. Co-delivery of rapamycin-and piperine-loaded polymeric nanoparticles for breast cancer treatment. Drug Deliv. 2016; 23(7): 2608-2616. [CrossRef]
29. [29] Khan W, Kumar N. Drug targeting to macrophages using paromomycin-loaded albumin microspheres for treatment of visceral leishmaniasis: An in vitro evaluation. J Drug Target. 2011; 19(4): 239-250. [CrossRef]