Microencapsulation of Lactobacillus plantarum FNCC 0026: Physical properties of microcapsules and antibacterial activity against multi-drug resistance organism (MDRO)

Safarini Marwah; Achmad Toto Poernomo; Nuzul Wahyuning Diyah; Ade Abiyyatun Mahdiyyah; Esti Hendradi

Microencapsulation of Lactobacillus plantarum FNCC 0026: Physical properties of microcapsules and antibacterial activity against multi-drug resistance organism (MDRO)

Abstract

This study aimed to evaluate the microparticle system of Lactobacillus plantarum FNCC 0026 in an optimal combination of a gelatin-alginate matrix. Four formulas with a variation of 2.5% alginate-gelatin polymer combination were encapsulated using the aerosolization technique. Microcapsule physical properties such as morphology, particle size distribution, swelling index, % mπoisture content, structural analysis by Fourier Transform Infrared (FTIR), as well as probiotic viability and antimicrobial activity in the matrix were investigated to evaluate the formulation. All formulations showed similarly uniform, spherical microparticles dispersed without agglomeration. The increased gelation concentration was accompanied by structural compactness as observed by scanning electron microscopy. Compared to free cells, the antibacterial activity increased against Methicillin Resistant Staphylococcus aureus (MRSA), and the inhibitory ability of microspheres against Extended Spectrum Beta Lactamase (ESBL) Escherichia coli. decreased. Under acidic conditions, gelatin can maintain the integrity of the capsule characterized by the absence of bacterial growth. Also, in alkaline conditions, the matrix swells well and has probiotic antibacterial activity. When stored, the microparticles could maintain probiotic function for up to 45 days. And from these studies, alginate gelatin matrix microspheres may form suitable capsules for Lactobacillus plantarum and probiotics could play an optimal role in inhibiting the growth of MRSA and ESBL E. coli.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry, Pharmaceutical Microbiology

Journal Section

Research Article

Authors

Safarini Marwah This is me
0000-0001-8491-5555
Indonesia

Achmad Toto Poernomo This is me
0000-0003-1845-2235
Indonesia

Nuzul Wahyuning Diyah This is me
0000-0001-6416-3982
Indonesia

Ade Abiyyatun Mahdiyyah This is me
0000-0001-9674-2858
Indonesia

Esti Hendradi ^*
0000-0002-8216-8549
Indonesia

Publication Date

June 28, 2025

Submission Date

November 4, 2022

Acceptance Date

March 19, 2023

Published in Issue

Year 2024 Volume: 28 Number: 2

IZ

https://izlik.org/JA69WY86HU

Cite

RIS / Bibtex

APA

Marwah, S., Poernomo, A. T., Diyah, N. W., Mahdiyyah, A. A., & Hendradi, E. (2025). Microencapsulation of Lactobacillus plantarum FNCC 0026: Physical properties of microcapsules and antibacterial activity against multi-drug resistance organism (MDRO). Journal of Research in Pharmacy, 28(2), 429-437. https://izlik.org/JA69WY86HU

AMA

1.Marwah S, Poernomo AT, Diyah NW, Mahdiyyah AA, Hendradi E. Microencapsulation of Lactobacillus plantarum FNCC 0026: Physical properties of microcapsules and antibacterial activity against multi-drug resistance organism (MDRO). J. Res. Pharm. 2025;28(2):429-437. https://izlik.org/JA69WY86HU

Chicago

Marwah, Safarini, Achmad Toto Poernomo, Nuzul Wahyuning Diyah, Ade Abiyyatun Mahdiyyah, and Esti Hendradi. 2025. “Microencapsulation of Lactobacillus Plantarum FNCC 0026: Physical Properties of Microcapsules and Antibacterial Activity Against Multi-Drug Resistance Organism (MDRO)”. Journal of Research in Pharmacy 28 (2): 429-37. https://izlik.org/JA69WY86HU.

EndNote

Marwah S, Poernomo AT, Diyah NW, Mahdiyyah AA, Hendradi E (June 1, 2025) Microencapsulation of Lactobacillus plantarum FNCC 0026: Physical properties of microcapsules and antibacterial activity against multi-drug resistance organism (MDRO). Journal of Research in Pharmacy 28 2 429–437.

IEEE

[1]S. Marwah, A. T. Poernomo, N. W. Diyah, A. A. Mahdiyyah, and E. Hendradi, “Microencapsulation of Lactobacillus plantarum FNCC 0026: Physical properties of microcapsules and antibacterial activity against multi-drug resistance organism (MDRO)”, J. Res. Pharm., vol. 28, no. 2, pp. 429–437, June 2025, [Online]. Available: https://izlik.org/JA69WY86HU

ISNAD

Marwah, Safarini - Poernomo, Achmad Toto - Diyah, Nuzul Wahyuning - Mahdiyyah, Ade Abiyyatun - Hendradi, Esti. “Microencapsulation of Lactobacillus Plantarum FNCC 0026: Physical Properties of Microcapsules and Antibacterial Activity Against Multi-Drug Resistance Organism (MDRO)”. Journal of Research in Pharmacy 28/2 (June 1, 2025): 429-437. https://izlik.org/JA69WY86HU.

JAMA

1.Marwah S, Poernomo AT, Diyah NW, Mahdiyyah AA, Hendradi E. Microencapsulation of Lactobacillus plantarum FNCC 0026: Physical properties of microcapsules and antibacterial activity against multi-drug resistance organism (MDRO). J. Res. Pharm. 2025;28:429–437.

MLA

Marwah, Safarini, et al. “Microencapsulation of Lactobacillus Plantarum FNCC 0026: Physical Properties of Microcapsules and Antibacterial Activity Against Multi-Drug Resistance Organism (MDRO)”. Journal of Research in Pharmacy, vol. 28, no. 2, June 2025, pp. 429-37, https://izlik.org/JA69WY86HU.

Vancouver

1.Safarini Marwah, Achmad Toto Poernomo, Nuzul Wahyuning Diyah, Ade Abiyyatun Mahdiyyah, Esti Hendradi. Microencapsulation of Lactobacillus plantarum FNCC 0026: Physical properties of microcapsules and antibacterial activity against multi-drug resistance organism (MDRO). J. Res. Pharm. [Internet]. 2025 Jun. 1;28(2):429-37. Available from: https://izlik.org/JA69WY86HU