Poly(3-methylthiophene) modified gold sensor for determination of 3,4-dihydroxyphenylacetic acid, ascorbic acid and uric acid in biological fluids using voltammetric techniques

Year 2019, Volume: 23 Issue: 1, 25 - 33, 27.06.2025

Ebru Kuyumcu Savan , Gamze Erdoğdu

https://izlik.org/JA37UW27FY

Abstract

Electrochemical polymerization was carried out in a one-compartment cell containing deaerated
acetonitrile, and 100 mM tetrabutylammonium tetrafluoroborate monomer of 3-methylthiophene. The polymer films
were grown on a gold electrode by bulk electrolysis at 1.700 V (vs. Ag/Ag+). The modified sensor showed an excellent
electrocatalytic effect towards oxidation of the mixture of 3,4-dihydroxyphenylacetic acid (DOPAC), ascorbic acid and
uric acid, which appeared at a pH range of 2-11 by differential pulse voltammetry (DPV ) techniques. The best results
for determination of DOPAC were obtained by DPV in PBS (pH 7.00). A calibration curve was obtained in the DOPAC
concentration range of 1.0-50.0 μM (r = 0.9948). Limit of detection and limit of quantification were found as 0.066 μM
and 0.220 μM, respectively. The results of the experiments indicated that the sensor has good stability, sensitivity and
reproducibility for at least one month if stored in dry air.

Keywords

Poly (3-methylthiophene) , 3 , 4-dihydroxyphenylacetic acid , ascorbic acid , uric acid , voltammetry

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