Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations

Emre Şefik Çağlar; Neslihan Üstündağ Okur; Yesim Karasulu

Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations

Abstract

It is an analytical requirement to identify and determine high molecular weight APIs in pharmaceutical forms. For this reason, it is aimed to develop and validate an analytical method for the determination of hyaluronic acid in pharmaceutical form in this study. 0.1 M Na2SO4 was prepared as mobile phase. The separation of compound was performed with a OHpak Shodex SB-806M HQ (13 μm, 8,0 x 300mm, Japan) analytical column. Guard column which is OHpak SB-G, (8 μm, 100 Å, 6.0 x 50 mm, Japan) was integrated to the system before the analytical column. UV detection at 198 nm was used to monitor the eluent and flow rate of the mobile phase was set to 1 mL/min. The method has been validated in terms of system suitability, linearity, limits of detection (LOD) and quantity (LOQ), precision, accuracy, specificity, selectivity, and stability. The obtained findings showed that the analytical method has linearity higher than 0.99, accuracy, precision, selectivity and stability. The method was found to be precise, accurate and specific during the study.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Sciences

Journal Section

Research Article

Authors

Emre Şefik Çağlar
Türkiye

Neslihan Üstündağ Okur ^*
Türkiye

Yesim Karasulu
Türkiye

Publication Date

June 28, 2025

Submission Date

March 3, 2021

Acceptance Date

September 7, 2021

Published in Issue

Year 2022 Volume: 26 Number: 1

IZ

https://izlik.org/JA86PR56ZL

Cite

RIS / Bibtex

APA

Çağlar, E. Ş., Üstündağ Okur, N., & Karasulu, Y. (2025). Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations. Journal of Research in Pharmacy, 26(1), 112-122. https://izlik.org/JA86PR56ZL

AMA

1.Çağlar EŞ, Üstündağ Okur N, Karasulu Y. Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations. J. Res. Pharm. 2025;26(1):112-122. https://izlik.org/JA86PR56ZL

Chicago

Çağlar, Emre Şefik, Neslihan Üstündağ Okur, and Yesim Karasulu. 2025. “Development and Validation of an HPLC Method for the Determination of Hyaluronic Acid Active Substance in Pharmaceutical Formulations”. Journal of Research in Pharmacy 26 (1): 112-22. https://izlik.org/JA86PR56ZL.

EndNote

Çağlar EŞ, Üstündağ Okur N, Karasulu Y (June 1, 2025) Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations. Journal of Research in Pharmacy 26 1 112–122.

IEEE

[1]E. Ş. Çağlar, N. Üstündağ Okur, and Y. Karasulu, “Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations”, J. Res. Pharm., vol. 26, no. 1, pp. 112–122, June 2025, [Online]. Available: https://izlik.org/JA86PR56ZL

ISNAD

Çağlar, Emre Şefik - Üstündağ Okur, Neslihan - Karasulu, Yesim. “Development and Validation of an HPLC Method for the Determination of Hyaluronic Acid Active Substance in Pharmaceutical Formulations”. Journal of Research in Pharmacy 26/1 (June 1, 2025): 112-122. https://izlik.org/JA86PR56ZL.

JAMA

1.Çağlar EŞ, Üstündağ Okur N, Karasulu Y. Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations. J. Res. Pharm. 2025;26:112–122.

MLA

Çağlar, Emre Şefik, et al. “Development and Validation of an HPLC Method for the Determination of Hyaluronic Acid Active Substance in Pharmaceutical Formulations”. Journal of Research in Pharmacy, vol. 26, no. 1, June 2025, pp. 112-2, https://izlik.org/JA86PR56ZL.

Vancouver

1.Emre Şefik Çağlar, Neslihan Üstündağ Okur, Yesim Karasulu. Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(1):112-2. Available from: https://izlik.org/JA86PR56ZL