Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma

Fulchan Ali; Sk Habibullah; Yashwant Giri; Amulyaratna Behera; Biswaranjan Mohanty

Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma

Abstract

The present research work was proposed to develop a pH triggered in-situ gel formulation for the ocular delivery of acetazolamide to treat glaucoma. Carbopol-934, Carbopol 940, Hydroxypropyl methylcellulose (HPMC) K4M, and Sodium Alginate were chosen for the development of in-situ gel. Formulation with Carbopol 940 and sodium alginate was optimized by assessing the gelling capacity. The prepared in-situ gel formulations were thoroughly characterized for gelling time, gelation temperature, Fourier-transform infrared (FTIR) spectroscopy studies, rheological study, sterility testing, corneal drug permeation, ocular irritation test, and accelerated stability study. Ex-vivo corneal permeation study was performed using goat cornea. The result of transcorneal permeation of acetazolamide followed the Fickian diffusion process. The optimized formulations showed satisfactory gelling time (4.17-5.17 sec) and dissolution time (120 min). FTIR study confirms the compatibility between the polymers and acetazolamide. The sterility study showed a satisfactory result. After 72 h observation in the in-vivo rabbit eye irritation study, the eyes appeared normal. There were no significant changes in pH and drug content in the accelerated stability studies of the formulations.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Delivery Technologies

Journal Section

Research Article

Authors

Fulchan Ali This is me
0000-0001-9320-6961
India

Sk Habibullah
0000-0002-3646-7177
India

Yashwant Giri This is me
0000-0002-3162-6853
India

Amulyaratna Behera This is me
0000-0003-1779-4364
India

Biswaranjan Mohanty ^* This is me
0000-0003-3793-7668
India

Publication Date

June 28, 2025

Submission Date

May 23, 2022

Acceptance Date

August 14, 2022

Published in Issue

Year 2023 Volume: 27 Number: 1

IZ

https://izlik.org/JA28DX34WB

Cite

RIS / Bibtex

APA

Ali, F., Habibullah, S., Giri, Y., Behera, A., & Mohanty, B. (2025). Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma. Journal of Research in Pharmacy, 27(1), 74-85. https://izlik.org/JA28DX34WB

AMA

1.Ali F, Habibullah S, Giri Y, Behera A, Mohanty B. Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma. J. Res. Pharm. 2025;27(1):74-85. https://izlik.org/JA28DX34WB

Chicago

Ali, Fulchan, Sk Habibullah, Yashwant Giri, Amulyaratna Behera, and Biswaranjan Mohanty. 2025. “Formulation and Evaluation of Acetazolamide Loaded In–situ Gel for the Treatment of Glaucoma”. Journal of Research in Pharmacy 27 (1): 74-85. https://izlik.org/JA28DX34WB.

EndNote

Ali F, Habibullah S, Giri Y, Behera A, Mohanty B (June 1, 2025) Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma. Journal of Research in Pharmacy 27 1 74–85.

IEEE

[1]F. Ali, S. Habibullah, Y. Giri, A. Behera, and B. Mohanty, “Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma”, J. Res. Pharm., vol. 27, no. 1, pp. 74–85, June 2025, [Online]. Available: https://izlik.org/JA28DX34WB

ISNAD

Ali, Fulchan - Habibullah, Sk - Giri, Yashwant - Behera, Amulyaratna - Mohanty, Biswaranjan. “Formulation and Evaluation of Acetazolamide Loaded In–situ Gel for the Treatment of Glaucoma”. Journal of Research in Pharmacy 27/1 (June 1, 2025): 74-85. https://izlik.org/JA28DX34WB.

JAMA

1.Ali F, Habibullah S, Giri Y, Behera A, Mohanty B. Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma. J. Res. Pharm. 2025;27:74–85.

MLA

Ali, Fulchan, et al. “Formulation and Evaluation of Acetazolamide Loaded In–situ Gel for the Treatment of Glaucoma”. Journal of Research in Pharmacy, vol. 27, no. 1, June 2025, pp. 74-85, https://izlik.org/JA28DX34WB.

Vancouver

1.Fulchan Ali, Sk Habibullah, Yashwant Giri, Amulyaratna Behera, Biswaranjan Mohanty. Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(1):74-85. Available from: https://izlik.org/JA28DX34WB