In vitro genotoxic and antigenotoxic effects of delphinidin chloride on human peripheral blood lymphocytes

Mehmet Tahir Hüsunet; Hasan Basri İla

In vitro genotoxic and antigenotoxic effects of delphinidin chloride on human peripheral blood lymphocytes

Abstract

Delphinidin is an anthocyanidin which is found in fruits and vegetables as a primary plant pigment used for various purposes. The purpose of this study was to investigate the in vitro genotoxic and antigenotoxic effects of delphinidin chloride (DC) in human peripheral blood lymphocytes. Final concentrations of 25 μM, 50 μM, 75 μM and 100 μM of DC were tested for 24 or 48 hours treatment periods. For detection of possible antigenotoxic potential of DC, human peripheral lymphocytes were co-treated with DC and a known clastogenic agent mitomycin-C (MMC). Genotoxic and antigenotoxic effects of DC were determined with the chromosome aberration (CA) and micronucleus (MN) tests. Cytotoxic effect of DC was determined by measuring the nuclear division index (NDI) and mitotic index (MI). Additionally, total oxidant and antioxidant values were determined by a spectrophotometric method. CA variations resulting from DC treatments did not reveal statistical significance as compared with controls. In tubes treated with DC and MMC together, DC significantly decreased the CA frequency caused by MMC (P≤0.01). This decrease was almost 50% as compared to the positive control MMC. In this study, DC alone did not lead to the CA and MN formation in all culture tubes. DC did not cause a significant oxidative stress. DC has an antigenotoxic effect against the mutagenic effects of MMC.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Mehmet Tahir Hüsunet ^*
0000-0003-1424-5132
Türkiye

Hasan Basri İla
0000-0002-3221-8587
Türkiye

Publication Date

June 27, 2025

Submission Date

September 14, 2017

Acceptance Date

January 6, 2018

Published in Issue

Year 2018 Volume: 22 Number: 2

IZ

https://izlik.org/JA26CB32EG

Cite

RIS / Bibtex

APA

Hüsunet, M. T., & İla, H. B. (2025). In vitro genotoxic and antigenotoxic effects of delphinidin chloride on human peripheral blood lymphocytes. Journal of Research in Pharmacy, 22(2), 209-217. https://izlik.org/JA26CB32EG

AMA

1.Hüsunet MT, İla HB. In vitro genotoxic and antigenotoxic effects of delphinidin chloride on human peripheral blood lymphocytes. J. Res. Pharm. 2025;22(2):209-217. https://izlik.org/JA26CB32EG

Chicago

Hüsunet, Mehmet Tahir, and Hasan Basri İla. 2025. “In Vitro Genotoxic and Antigenotoxic Effects of Delphinidin Chloride on Human Peripheral Blood Lymphocytes”. Journal of Research in Pharmacy 22 (2): 209-17. https://izlik.org/JA26CB32EG.

EndNote

Hüsunet MT, İla HB (June 1, 2025) In vitro genotoxic and antigenotoxic effects of delphinidin chloride on human peripheral blood lymphocytes. Journal of Research in Pharmacy 22 2 209–217.

IEEE

[1]M. T. Hüsunet and H. B. İla, “In vitro genotoxic and antigenotoxic effects of delphinidin chloride on human peripheral blood lymphocytes”, J. Res. Pharm., vol. 22, no. 2, pp. 209–217, June 2025, [Online]. Available: https://izlik.org/JA26CB32EG

ISNAD

Hüsunet, Mehmet Tahir - İla, Hasan Basri. “In Vitro Genotoxic and Antigenotoxic Effects of Delphinidin Chloride on Human Peripheral Blood Lymphocytes”. Journal of Research in Pharmacy 22/2 (June 1, 2025): 209-217. https://izlik.org/JA26CB32EG.

JAMA

1.Hüsunet MT, İla HB. In vitro genotoxic and antigenotoxic effects of delphinidin chloride on human peripheral blood lymphocytes. J. Res. Pharm. 2025;22:209–217.

MLA

Hüsunet, Mehmet Tahir, and Hasan Basri İla. “In Vitro Genotoxic and Antigenotoxic Effects of Delphinidin Chloride on Human Peripheral Blood Lymphocytes”. Journal of Research in Pharmacy, vol. 22, no. 2, June 2025, pp. 209-17, https://izlik.org/JA26CB32EG.

Vancouver

1.Mehmet Tahir Hüsunet, Hasan Basri İla. In vitro genotoxic and antigenotoxic effects of delphinidin chloride on human peripheral blood lymphocytes. J. Res. Pharm. [Internet]. 2025 Jun. 1;22(2):209-17. Available from: https://izlik.org/JA26CB32EG