Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment

Gülşah Erel Akbaba; Hasan Akbaba; A. Gülten Kantarcı

Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment

Abstract

In this study, we aimed to develop a novel positive charged nucleic acid delivery system for the treatment of glioblastoma. For this purpose, Epidermal Growth Factor Receptor (EGFR) which plays a prominent role in glioblastoma was selected as a target. Cationic solid lipid nanoparticles (cSLN) were developed by microemulsion dilution method using cetyl palmitate as matrix lipid, Cremephor RH40 and Peceol as surfactants, and ethanol as cosurfactant. Characterization studies showed that obtained nanoparticles are positively charged and has an appropriate particle size for nucleic acid delivery (<20 nm). Gel retardation assay revealed that cSLNs have complexation ability with siRNA EGFR (cSLN:siRNAEGFR) and this complex is able to protect siRNA from serum‐ mediated degradation up to 6 h. The cytotoxicity evaluation of nanoparticles performed on U87 Human Glioblastoma Cell Line. Furthermore, in vitro delivery of siRNA-EGFR via cSLN inhibited EGFR expression significantly at 50nM siRNA dose compared to free siRNA-EGFR at the same dose on U87 cell line (p<0.05). Based on these findings, we propose that the developed cSLN system may has a potential as a siRNA delivery system for glioblastoma.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biotechnology

Journal Section

Research Article

Authors

Gülşah Erel Akbaba
0000-0003-3287-5277
Türkiye

Hasan Akbaba
0000-0001-9273-6346
Türkiye

A. Gülten Kantarcı ^* This is me
0000-0001-8813-5353
Türkiye

Publication Date

June 27, 2025

Submission Date

November 21, 2017

Acceptance Date

January 2, 2018

Published in Issue

Year 2018 Volume: 22 Number: 2

IZ

https://izlik.org/JA58GX47JP

Cite

RIS / Bibtex

APA

Erel Akbaba, G., Akbaba, H., & Kantarcı, A. G. (2025). Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment. Journal of Research in Pharmacy, 22(2), 299-306. https://izlik.org/JA58GX47JP

AMA

1.Erel Akbaba G, Akbaba H, Kantarcı AG. Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment. J. Res. Pharm. 2025;22(2):299-306. https://izlik.org/JA58GX47JP

Chicago

Erel Akbaba, Gülşah, Hasan Akbaba, and A. Gülten Kantarcı. 2025. “Development and in Vitro Evaluation of Positive-Charged Solid Lipid Nanoparticles As Nucleic Acid Delivery System in Glioblastoma Treatment”. Journal of Research in Pharmacy 22 (2): 299-306. https://izlik.org/JA58GX47JP.

EndNote

Erel Akbaba G, Akbaba H, Kantarcı AG (June 1, 2025) Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment. Journal of Research in Pharmacy 22 2 299–306.

IEEE

[1]G. Erel Akbaba, H. Akbaba, and A. G. Kantarcı, “Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment”, J. Res. Pharm., vol. 22, no. 2, pp. 299–306, June 2025, [Online]. Available: https://izlik.org/JA58GX47JP

ISNAD

Erel Akbaba, Gülşah - Akbaba, Hasan - Kantarcı, A. Gülten. “Development and in Vitro Evaluation of Positive-Charged Solid Lipid Nanoparticles As Nucleic Acid Delivery System in Glioblastoma Treatment”. Journal of Research in Pharmacy 22/2 (June 1, 2025): 299-306. https://izlik.org/JA58GX47JP.

JAMA

1.Erel Akbaba G, Akbaba H, Kantarcı AG. Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment. J. Res. Pharm. 2025;22:299–306.

MLA

Erel Akbaba, Gülşah, et al. “Development and in Vitro Evaluation of Positive-Charged Solid Lipid Nanoparticles As Nucleic Acid Delivery System in Glioblastoma Treatment”. Journal of Research in Pharmacy, vol. 22, no. 2, June 2025, pp. 299-06, https://izlik.org/JA58GX47JP.

Vancouver

1.Gülşah Erel Akbaba, Hasan Akbaba, A. Gülten Kantarcı. Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment. J. Res. Pharm. [Internet]. 2025 Jun. 1;22(2):299-306. Available from: https://izlik.org/JA58GX47JP