Hypoxic mesenchymal stem cells reduce collagen loss caused by UVB exposure by activating Nrf2 and GPx

Vira Avianti Gunawan; Titiek Sumarawati; Agung Putra; Eko Setiawan; Setyo Trisnadi; Chodijah Chodijah; Nur Dina Amalina

doi:10.12991/jrespharm.1684891

Hypoxic mesenchymal stem cells reduce collagen loss caused by UVB exposure by activating Nrf2 and GPx

Abstract

UVB radiation causes collagen loss due to increased oxidative stress, suppressing GPx levels through Nrf2. This study aims to analyze the effect of H-MSCs on Nrf2 gene expression and GPx levels in male Wistar rats with UVB-induced collagen loss. This experimental study employed a post-test-only control group design with a completely randomized arrangement. Twenty-five male Wistar rats were divided into 5 groups. Group 1 received no treatment, Group 2 was exposed to UVB and given 200μL NaCl, Group 3 was exposed to UVB and given 200μL HA, while Groups 4 and 5 were exposed to UVB and treated with H-MSCs at doses of 2.5x10^5 and 5x10^5 cells in 200μL NaCl, respectively. Groups 2 through 5 received UVB irradiation at a dose of 160 mJ/cm2, 5 times a week for 2 weeks on the dorsal skin. Treatments were administered on day 15, and Nrf2 gene expression was analyzed using qRT-PCR, while GPx levels were measured by ELISA on day 22. Data analysis included normality and homogeneity tests, followed by difference tests using SPSS. Administration of H-MSCs at doses 2.5x10^5 and 5x10^5 cells significantly induced Nrf2 gene expression ratios to 1.41±0.21 and 1.61±0.19, respectively, compared to the negative control group (0.80±0.21). The Nrf2 gene expression was increased on corresponded with elevated GPx levels, with the 5x10^5 cell H-MSCs group exhibiting the highest GPx level at 94.88±5.34 pg/mL, significantly different from the negative control group, which had the lowest GPx level at 36.88±3.22 pg/mL. H-MSCs administration can ameliorate UVB-induced collagen loss by increasing Nrf2 gene expression and GPx levels.

Keywords

Project Number

None

Ethical Statement

The study was approved by the ethics committee of the Medical Faculty, Universitas Islam Sultan Agung, Semarang, Indonesia, under No. 407/X/024/Komisi Bioetik, dated October 30, 2024.

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Details

Primary Language

English

Subjects

Medical Pharmacology

Journal Section

Research Article

Authors

Vira Avianti Gunawan This is me
Indonesia

Titiek Sumarawati This is me
Indonesia

Agung Putra This is me
Indonesia

Eko Setiawan This is me
Indonesia

Setyo Trisnadi This is me
Indonesia

Chodijah Chodijah This is me
Indonesia

Nur Dina Amalina ^*
Indonesia

Publication Date

October 6, 2026

Submission Date

April 27, 2025

Acceptance Date

June 25, 2026

Published in Issue

Year 2026 Volume: 30 Number: 5

DOI

https://doi.org/10.12991/jrespharm.1684891

IZ

https://izlik.org/JA77HH82UY

Cite

RIS / Bibtex

APA

Avianti Gunawan, V., Sumarawati, T., Putra, A., Setiawan, E., Trisnadi, S., Chodijah, C., & Amalina, N. D. (2026). Hypoxic mesenchymal stem cells reduce collagen loss caused by UVB exposure by activating Nrf2 and GPx. Journal of Research in Pharmacy, 30(5), 1396-1409. https://doi.org/10.12991/jrespharm.1684891

AMA

1.Avianti Gunawan V, Sumarawati T, Putra A, et al. Hypoxic mesenchymal stem cells reduce collagen loss caused by UVB exposure by activating Nrf2 and GPx. J. Res. Pharm. 2026;30(5):1396-1409. doi:10.12991/jrespharm.1684891

Chicago

Avianti Gunawan, Vira, Titiek Sumarawati, Agung Putra, et al. 2026. “Hypoxic Mesenchymal Stem Cells Reduce Collagen Loss Caused by UVB Exposure by Activating Nrf2 and GPx”. Journal of Research in Pharmacy 30 (5): 1396-1409. https://doi.org/10.12991/jrespharm.1684891.

EndNote

Avianti Gunawan V, Sumarawati T, Putra A, Setiawan E, Trisnadi S, Chodijah C, Amalina ND (October 1, 2026) Hypoxic mesenchymal stem cells reduce collagen loss caused by UVB exposure by activating Nrf2 and GPx. Journal of Research in Pharmacy 30 5 1396–1409.

IEEE

[1]V. Avianti Gunawan et al., “Hypoxic mesenchymal stem cells reduce collagen loss caused by UVB exposure by activating Nrf2 and GPx”, J. Res. Pharm., vol. 30, no. 5, pp. 1396–1409, Oct. 2026, doi: 10.12991/jrespharm.1684891.

ISNAD

Avianti Gunawan, Vira - Sumarawati, Titiek - Putra, Agung - Setiawan, Eko - Trisnadi, Setyo - Chodijah, Chodijah - Amalina, Nur Dina. “Hypoxic Mesenchymal Stem Cells Reduce Collagen Loss Caused by UVB Exposure by Activating Nrf2 and GPx”. Journal of Research in Pharmacy 30/5 (October 1, 2026): 1396-1409. https://doi.org/10.12991/jrespharm.1684891.

JAMA

1.Avianti Gunawan V, Sumarawati T, Putra A, Setiawan E, Trisnadi S, Chodijah C, Amalina ND. Hypoxic mesenchymal stem cells reduce collagen loss caused by UVB exposure by activating Nrf2 and GPx. J. Res. Pharm. 2026;30:1396–1409.

MLA

Avianti Gunawan, Vira, et al. “Hypoxic Mesenchymal Stem Cells Reduce Collagen Loss Caused by UVB Exposure by Activating Nrf2 and GPx”. Journal of Research in Pharmacy, vol. 30, no. 5, Oct. 2026, pp. 1396-09, doi:10.12991/jrespharm.1684891.

Vancouver

1.Vira Avianti Gunawan, Titiek Sumarawati, Agung Putra, Eko Setiawan, Setyo Trisnadi, Chodijah Chodijah, Nur Dina Amalina. Hypoxic mesenchymal stem cells reduce collagen loss caused by UVB exposure by activating Nrf2 and GPx. J. Res. Pharm. 2026 Oct. 1;30(5):1396-409. doi:10.12991/jrespharm.1684891