Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell

Demet Çelebi; Özgür Çelebi; Sümeyye Başer; Ali Taghizadehghalehjoughi

doi:10.29228/jrp.516

Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell

Abstract

Liver abscesses are most commonly attributed to Escherichia coli and Klebsiella pneumoniae, but because Streptococcus mitis is a commensal bacterium with low virulence activity, it is overlooked by its antimicrobial resistance and virulence factors. In this study, we tried to evaluate the synergistic effect of boron compounds and the effect on biofilm in the infection model we created with Streptococcus mitis NCTC 12261 on the HepG2 liver cell line. Minimal inhibitory concentration (MIC) values were determined on Streptococcus mitis at doses of 31.25 µg/ml of Sodium Perborate Metahydrate (SPM), Zinc Borate (ZB) 62.5 µg/ml and Etidote 125 µg/ml. Etidote+SPM, Etidote+ZB, and ZB+SPM combinations showed the most effective fractional inhibitor concentration value synergistic effect at minimum doses of 32 µg/ml+32 µg/ml in all combinations, while it showed an effect on biofilm at doses of 1028 µg/ml+ 1028 µg/ml HepG2 Cell culture.

Keywords

References

[1] Marron A, Carratalà J, González-Barca E, Fernández-Sevilla A, Alcaide F, Gudiol F. Serious complications of bacteremia caused by viridans streptococci in neutropenic patients with cancer. Clin Infect Dis. 2000; 31: 1126–1130. https://doi.org/10.1086/317460.
[2] Ahmed R, Hassall T, Morland B, Gray J. Viridans streptococcus bacteremia in children on chemotherapy for cancer: an underestimated problem. Pediatr Hematol Oncol. 2003; 20: 439–444. https://doi.org/10.1080/08880010390220144.
[3] Hoshino T, Fujiwara T, Kilian M. Use of phylogenetic and phenotypic analyses to identify nonhemolytic streptococci isolated from bacteremic patients. J Clin Microbiol. 2005; 43: 6073–6085. https://doi.org/10.1128/JCM.43.12.6073-6085.2005.
[4] Husain E, Whitehead S, Castell A, Thomas EE, Speert DP. Viridans streptococci bacteremia in children with malignancy: relevance of species identification and penicillin susceptibility. Pediatr Infect Dis J. 2005; 24: 563–566. https://doi.org/10.1097/01.inf.0000164708.21464.03.
[5] Han XY, Kamana M, Rolston KV. Viridans streptococci isolated by culture from blood of cancer patients: clinical and microbiologic analysis of 50 cases. J Clin Microbiol. 2006; 44: 160–165. https://doi.org/10.1128/JCM.44.1.160-165.2006.
[6] Kohno K, Nagafuji K, Tsukamoto H, Horiuchi T, Takase K, Aoki K, Henzan H, Kamezaki K, Takenaka K, Miyamoto T, Teshima T, Harada M, Akashi K. Infectious complications in patients receiving autologous CD34-selected hematopoietic stem cell transplantation for severe autoimmune diseases. Transpl Infect Dis. 2009; 11: 318–323. https://doi.org/10.1111/j.1399-3062.2009.00401.x.
[7] Mitchell J. Streptococcus mitis: Walking the line between commensalism and pathogenesis. Mol Oral Microbiol. 2011; 26(2): 89–98. https://doi.org/10.1111/j.2041-1014.2010.0601.x.
[8] HPA. Pyogenic and non-pyogenic streptococcal bacteraemias, England, Wales and Northern Ireland: 2008. Health Protection Report [serial online]. 2009; 3(49).

[9] Bochud PY, Cometta A, Francioli P. Virulent infections caused by alpha-haemolytic streptococci in cancer patients and their management. Curr Opin Infect Dis. 1997; 10: 422–430. https://doi.org/10.1128/jcm.37.6.1876-1880.1999.
[10] Coghi PS, Zhu Y, Xie H, Hosmane NS, Zhang Y. Organoboron Compounds: Effective antibacterial and antiparasitic agents. Molecules. 2021; 26(11): 3309. https://doi.org/10.3390/molecules26113309.
[11] Lamas CC, Eykyn SJ. Blood culture negative endocarditis: analysis of 63 cases presenting over 25 years. Heart. 2003; 89: 258–262. https://doi.org/10.1136/heart.89.3.258.
[12] Kutlu SS, Sacar S, Cevahir N, Turgut HI. Community-acquired Streptococcus mitis meningitis: a case report. Int J Infect Dis. 2008; 12: 107–109. https://doi.org/10.1016/j.ijid.2008.01.003.
[13] Johannsen EC, Sifri CD, Madoff LC. Pyogenic liver abscesses. Infect Dis Clin North Am. 2000; 14: 547–563. https://doi.org/10.1016/s0891-5520(05)70120-3.
[14] Duguid R. Copper-inhibition of the growth of oral streptococci and actinomyces. Biomaterials. 1983; 4(3): 225–227. https://doi.org/10.1016/0142-9612(83)90017-0.
[15] Seppala H, Haanpera M, Al-Juhaish M, Jarvinen H, Jalava J, Huovinen P. Antimicrobial susceptibility patterns and macrolide resistance genes of viridans group streptococci from normal flora. J Antimicrob Chemother. 2003; 52(4): 636–644. https://doi.org/10.1093/jac/dkg423.
[16] Yiş R, Yüksel CN, Derundere U, Yiş U. Meningitis and white matter lesions due to Streptococcus mitis in a previously healthy child. Microbiol Bull. 2011; 45(4): 741–745.
[17] https://www.heraproject.com/files/7-f-04-hera%20sodium%20perborate%20full%20web%20wd.pdf. Erişim Tarihi: 16.01.2023.
[18] Celebi D, Taghizadehghalehjoughi A, Baser S, Genc S, Yilmaz A, Yeni Y, Yesilyurt F, Yildirim S, Bolat I, Kordali S, Yilmaz F, Hacimuftuoglu A, Celebi O, Margina D, Nitulescu GM, Spandidos DA, Tsatsakis A. Effects of boric acid and potassium metaborate on cytokine levels and redox stress parameters in a wound model infected with methicillin‑resistant Staphylococcus aureus. Mol Med Rep. 2022; 26(3): 294. https://doi.org/10.3892/mmr.2022.12809.

Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Demet Çelebi ^*
0000-0002-2355-0561
Türkiye

Özgür Çelebi
0000-0003-4578-9474
Türkiye

Sümeyye Başer
Türkiye

Ali Taghizadehghalehjoughi
0000-0002-3506-0324
Türkiye

Publication Date

June 28, 2025

Submission Date

January 17, 2023

Acceptance Date

April 11, 2023

Published in Issue

Year 2023 Volume: 27 Number: 6

DOI

https://doi.org/10.29228/jrp.516

IZ

https://izlik.org/JA39SM26DL

Cite

RIS / Bibtex

APA

Çelebi, D., Çelebi, Ö., Başer, S., & Taghizadehghalehjoughi, A. (2025). Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell. Journal of Research in Pharmacy, 27(6), 2277-2284. https://doi.org/10.29228/jrp.516

AMA

1.Çelebi D, Çelebi Ö, Başer S, Taghizadehghalehjoughi A. Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell. J. Res. Pharm. 2025;27(6):2277-2284. doi:10.29228/jrp.516

Chicago

Çelebi, Demet, Özgür Çelebi, Sümeyye Başer, and Ali Taghizadehghalehjoughi. 2025. “Investigation of the Antibacterial, Antibiofilm and Cytotoxic Effects of Boron Compounds in a Streptococcus Mitis Infection Model on HepG2 Liver Cell”. Journal of Research in Pharmacy 27 (6): 2277-84. https://doi.org/10.29228/jrp.516.

EndNote

Çelebi D, Çelebi Ö, Başer S, Taghizadehghalehjoughi A (July 1, 2025) Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell. Journal of Research in Pharmacy 27 6 2277–2284.

IEEE

[1]D. Çelebi, Ö. Çelebi, S. Başer, and A. Taghizadehghalehjoughi, “Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell”, J. Res. Pharm., vol. 27, no. 6, pp. 2277–2284, July 2025, doi: 10.29228/jrp.516.

ISNAD

Çelebi, Demet - Çelebi, Özgür - Başer, Sümeyye - Taghizadehghalehjoughi, Ali. “Investigation of the Antibacterial, Antibiofilm and Cytotoxic Effects of Boron Compounds in a Streptococcus Mitis Infection Model on HepG2 Liver Cell”. Journal of Research in Pharmacy 27/6 (July 1, 2025): 2277-2284. https://doi.org/10.29228/jrp.516.

JAMA

1.Çelebi D, Çelebi Ö, Başer S, Taghizadehghalehjoughi A. Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell. J. Res. Pharm. 2025;27:2277–2284.

MLA

Çelebi, Demet, et al. “Investigation of the Antibacterial, Antibiofilm and Cytotoxic Effects of Boron Compounds in a Streptococcus Mitis Infection Model on HepG2 Liver Cell”. Journal of Research in Pharmacy, vol. 27, no. 6, July 2025, pp. 2277-84, doi:10.29228/jrp.516.

Vancouver

1.Demet Çelebi, Özgür Çelebi, Sümeyye Başer, Ali Taghizadehghalehjoughi. Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell. J. Res. Pharm. 2025 Jul. 1;27(6):2277-84. doi:10.29228/jrp.516

Cited By

Antimicrobial potential of boron-containing compounds: Antibacterial, antifungal, and antimycobacterial activities

Journal of Boron

https://doi.org/10.30728/boron.1693877