In vitro antiproliferative, antioxidant and anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz

Aybeniz Yildirim; Ali Şen; Fatih Göger; Özlem Bingöl Özakpınar; Leyla Bitiş

In vitro antiproliferative, antioxidant and anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz

Abstract

In this study, methanol extract (CSM) and their hexane (CSH), chloroform (CSC), ethyl acetate (CSEA) and aqueous methanol fractions (CSAM) prepared from aerial parts of Centaurea saligna (K.Koch) Wagenitz were investigated for in vitro antiproliferative, anti-inflammatory and antioxidant activity. Anticancer, antioxidant, antiinflammatory activities of these extracts were carried out by MTT, DPPH-ABTS, 5-lipoxygenase methods, respectively. Methanol extract and its fractions of C. saligna were evaluated for their cytotoxicity against MCF-7, A549, HeLa, HT-29, PC-3 cell lines at a concentration of 50 µg / ml. Total flavonoid and phenolic contents of extracts were detected by AlCI3 and Folin-Ciocalteu methods, respectively. Analysis of phytochemical of CSEA, showing a strong anti-inflammatory activity with good antioxidant activity, was performed by LC-MS/MS. CSC exhibited the best antiproliferative activity against HeLa, HT-29, MCF-7 cell lines with 50.18%, 46.88%, 45.42% mortality, respectively. CSEA showed the highest antioxidant activity with IC50 values of 82.05 µg/ml and 108.4 µg/ml against ABTS and DPPH radicals, respectively. The highest total phenolic amounts have been determined in CSEA with value of 379.2 mg GAE/g extract. In the same way, the highest total flavonoid amounts have been observed in CSEA with values of 170.3 mg QE/g extract. CSEA showed strong anti-inflammatory activity with IC50 value of 0.10 µg/ml when compared to indomethacine as standard (22.39 µg/ml). Analysis of CSEA by LC-MS/MS revealed that the major compounds were quinic acid, 5-caffeoylquinic acid, apigenin C-hexoside-C-pentoside, p-coumaroylquinic acid, quercetin glucoside, di-caffeoylquinic acid, isorhamnetine glucoside, isorhamnetin glucuronide and isorhamnetin derivative. These results proved that CSEA has significant anti-inflammatory and antioxidant activity and CSC has good antiproliferatif activity. Also, the results demostrate that CSEA and CSC are a good source for further bioactivity-guided isolation in discovering new active antioxidant, anti-inflammatory and antiproliferative compounds.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry, Pharmacognosy

Journal Section

Research Article

Authors

Aybeniz Yildirim
Türkiye

Ali Şen
Türkiye

Fatih Göger
Türkiye

Özlem Bingöl Özakpınar
Türkiye

Leyla Bitiş ^*
Türkiye

Publication Date

June 28, 2025

Submission Date

June 14, 2021

Acceptance Date

October 25, 2021

Published in Issue

Year 2022 Volume: 26 Number: 1

IZ

https://izlik.org/JA48XT99CH

Cite

RIS / Bibtex

APA

Yildirim, A., Şen, A., Göger, F., Bingöl Özakpınar, Ö., & Bitiş, L. (2025). In vitro antiproliferative, antioxidant and anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. Journal of Research in Pharmacy, 26(1), 163-173. https://izlik.org/JA48XT99CH

AMA

1.Yildirim A, Şen A, Göger F, Bingöl Özakpınar Ö, Bitiş L. In vitro antiproliferative, antioxidant and anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. J. Res. Pharm. 2025;26(1):163-173. https://izlik.org/JA48XT99CH

Chicago

Yildirim, Aybeniz, Ali Şen, Fatih Göger, Özlem Bingöl Özakpınar, and Leyla Bitiş. 2025. “In Vitro Antiproliferative, Antioxidant and Anti-Inflammatory Activities and Phenolic Profile of Centaurea Saligna (K.Koch) Wagenitz”. Journal of Research in Pharmacy 26 (1): 163-73. https://izlik.org/JA48XT99CH.

EndNote

Yildirim A, Şen A, Göger F, Bingöl Özakpınar Ö, Bitiş L (June 1, 2025) In vitro antiproliferative, antioxidant and anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. Journal of Research in Pharmacy 26 1 163–173.

IEEE

[1]A. Yildirim, A. Şen, F. Göger, Ö. Bingöl Özakpınar, and L. Bitiş, “In vitro antiproliferative, antioxidant and anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz”, J. Res. Pharm., vol. 26, no. 1, pp. 163–173, June 2025, [Online]. Available: https://izlik.org/JA48XT99CH

ISNAD

Yildirim, Aybeniz - Şen, Ali - Göger, Fatih - Bingöl Özakpınar, Özlem - Bitiş, Leyla. “In Vitro Antiproliferative, Antioxidant and Anti-Inflammatory Activities and Phenolic Profile of Centaurea Saligna (K.Koch) Wagenitz”. Journal of Research in Pharmacy 26/1 (June 1, 2025): 163-173. https://izlik.org/JA48XT99CH.

JAMA

1.Yildirim A, Şen A, Göger F, Bingöl Özakpınar Ö, Bitiş L. In vitro antiproliferative, antioxidant and anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. J. Res. Pharm. 2025;26:163–173.

MLA

Yildirim, Aybeniz, et al. “In Vitro Antiproliferative, Antioxidant and Anti-Inflammatory Activities and Phenolic Profile of Centaurea Saligna (K.Koch) Wagenitz”. Journal of Research in Pharmacy, vol. 26, no. 1, June 2025, pp. 163-7, https://izlik.org/JA48XT99CH.

Vancouver

1.Aybeniz Yildirim, Ali Şen, Fatih Göger, Özlem Bingöl Özakpınar, Leyla Bitiş. In vitro antiproliferative, antioxidant and anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(1):163-7. Available from: https://izlik.org/JA48XT99CH