Bioactivity potential and chemical profile of endophytic Stutzerimonas stutzeri strain D2 isolated from Myristica fatua Houtt

Jepri Agung Priyanto; Gian Primahana; Muhammad Eka Prastya; Desi Satiyaningsih; Ricson Pemimpin Hutagaol; Vera Permatasari; Megawati Megawati; Akhmad Darmawan

Bioactivity potential and chemical profile of endophytic Stutzerimonas stutzeri strain D2 isolated from Myristica fatua Houtt

Abstract

Endophytic bacteria isolated from medicinal plants are recognized as a source of valuable secondary metabolites. The present study aimed to isolate and evaluate the potential antibacterial, antioxidant, and cytotoxic properties of endophytic bacteria associated with Myristica fatua Houtt, along with molecular identification and chemical composition analysis of the most potential isolate. A total of 25 isolates were obtained from the root, leaf, and stem bark of M. fatua. 16 isolates (64%) exhibited antibacterial activity against at least one target strain. Preliminary screening showed that the D2 isolate displayed remarkable antibacterial activity against four targeted strains. D2- derived metabolite extract possessed moderate antibacterial activity with minimum inhibitory concentration (MIC) of 1,875 µg/mL for E. coli, P. aeruginosa, and S. aureus, and 3,750 µg/mL for B. subtilis, and minimum bactericidal concentration (MBC) of > 3,750 µg/mL. Furthermore, D2 extract exhibited weak antioxidant activity against DPPH (IC50: 242.61±3.2 µg/mL) and ABTS (IC50: 317.32±9.3 µg/mL). In the concentration of 100 µg/mL, the extract was able to decrease the viability of MCF-7 and HepG2 cells, with inhibition percentages of 52.6±9.7 and 41.5±9.36%, respectively. 16S rRNA-based identification confirmed D2 isolate was very similar (99.92%) to Stutzerimonas stutzeri strain SM12. The total phenolic content (TPC) and total flavonoid content (TFC) of the D2 extract were 21.11±2.3 mg GAE/g extract and 8.12±1.2 mg QE/g extract, respectively. At least fifteen volatile compounds were found in the D2 extract, as identified using GC-MS analysis. Four out of 15 compounds are well known responsible for antimicrobial, antioxidant, and cytotoxic properties, such as 2,4-di-tert-butyl phenol, pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), and bis(2-ethylhexyl) phthalate. The present study suggests that endophytic S. stutzeri strain D2 isolated from M. fatua Houtt could be a good source of active compounds valuable for pharmaceutical purposes.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Microbiology

Journal Section

Research Article

Authors

Jepri Agung Priyanto
0000-0003-2227-5040
Indonesia

Gian Primahana This is me
0000-0001-8176-3859
Indonesia

Muhammad Eka Prastya ^* This is me
0000-0003-2500-1264
Indonesia

Desi Satiyaningsih This is me
0009-0008-8280-8199
Indonesia

Ricson Pemimpin Hutagaol This is me
0009-0005-7034-6861
Indonesia

Vera Permatasari This is me
0000-0001-8460-6285
Indonesia

Megawati Megawati This is me
0000-0002-6698-0668
Indonesia

Akhmad Darmawan This is me
0000-0001-9352-5233
Indonesia

Publication Date

June 28, 2025

Submission Date

April 12, 2023

Acceptance Date

June 21, 2023

Published in Issue

Year 2024 Volume: 28 Number: 1

IZ

https://izlik.org/JA85YJ49KX

Cite

RIS / Bibtex

APA

Priyanto, J. A., Primahana, G., Prastya, M. E., Satiyaningsih, D., Hutagaol, R. P., Permatasari, V., Megawati, M., & Darmawan, A. (2025). Bioactivity potential and chemical profile of endophytic Stutzerimonas stutzeri strain D2 isolated from Myristica fatua Houtt. Journal of Research in Pharmacy, 28(1), 51-62. https://izlik.org/JA85YJ49KX

AMA

1.Priyanto JA, Primahana G, Prastya ME, et al. Bioactivity potential and chemical profile of endophytic Stutzerimonas stutzeri strain D2 isolated from Myristica fatua Houtt. J. Res. Pharm. 2025;28(1):51-62. https://izlik.org/JA85YJ49KX

Chicago

Priyanto, Jepri Agung, Gian Primahana, Muhammad Eka Prastya, et al. 2025. “Bioactivity Potential and Chemical Profile of Endophytic Stutzerimonas Stutzeri Strain D2 Isolated from Myristica Fatua Houtt”. Journal of Research in Pharmacy 28 (1): 51-62. https://izlik.org/JA85YJ49KX.

EndNote

Priyanto JA, Primahana G, Prastya ME, Satiyaningsih D, Hutagaol RP, Permatasari V, Megawati M, Darmawan A (June 1, 2025) Bioactivity potential and chemical profile of endophytic Stutzerimonas stutzeri strain D2 isolated from Myristica fatua Houtt. Journal of Research in Pharmacy 28 1 51–62.

IEEE

[1]J. A. Priyanto et al., “Bioactivity potential and chemical profile of endophytic Stutzerimonas stutzeri strain D2 isolated from Myristica fatua Houtt”, J. Res. Pharm., vol. 28, no. 1, pp. 51–62, June 2025, [Online]. Available: https://izlik.org/JA85YJ49KX

ISNAD

Priyanto, Jepri Agung - Primahana, Gian - Prastya, Muhammad Eka - Satiyaningsih, Desi - Hutagaol, Ricson Pemimpin - Permatasari, Vera - Megawati, Megawati - Darmawan, Akhmad. “Bioactivity Potential and Chemical Profile of Endophytic Stutzerimonas Stutzeri Strain D2 Isolated from Myristica Fatua Houtt”. Journal of Research in Pharmacy 28/1 (June 1, 2025): 51-62. https://izlik.org/JA85YJ49KX.

JAMA

1.Priyanto JA, Primahana G, Prastya ME, Satiyaningsih D, Hutagaol RP, Permatasari V, Megawati M, Darmawan A. Bioactivity potential and chemical profile of endophytic Stutzerimonas stutzeri strain D2 isolated from Myristica fatua Houtt. J. Res. Pharm. 2025;28:51–62.

MLA

Priyanto, Jepri Agung, et al. “Bioactivity Potential and Chemical Profile of Endophytic Stutzerimonas Stutzeri Strain D2 Isolated from Myristica Fatua Houtt”. Journal of Research in Pharmacy, vol. 28, no. 1, June 2025, pp. 51-62, https://izlik.org/JA85YJ49KX.

Vancouver

1.Jepri Agung Priyanto, Gian Primahana, Muhammad Eka Prastya, Desi Satiyaningsih, Ricson Pemimpin Hutagaol, Vera Permatasari, Megawati Megawati, Akhmad Darmawan. Bioactivity potential and chemical profile of endophytic Stutzerimonas stutzeri strain D2 isolated from Myristica fatua Houtt. J. Res. Pharm. [Internet]. 2025 Jun. 1;28(1):51-62. Available from: https://izlik.org/JA85YJ49KX