Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.)

Rian Oktiansyah; Elfita Elfita; Hary Wıdjajantı; Poedji Loekitowati Hariani; Nurlisa Hidayati; Arum Setıawan; Salni Salni

Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.)

Abstract

Infectious diseases are a global challenge today. One preventive measure is to maintain and improve humans’ immunity. Many people consume sungkai, a medicinal plant that is believed to cut down the exposure of severe covid-19 because it is related to antioxidant and antibacterial activity. This research investigated the antioxidant and antimicrobe agents of fungal endophyte extracts from the stem bark of sungkai. Endophytic fungal was isolated from the fresh tissue of the stem bark of sungkai and identified morphologically. The antioxidant of the endophytic fungi extract was tested with the DPPH, and antibacterial agent was tested by using the paper disc diffusion method. Molecular identification of endophytic fungi was done to extract that showed the most potential antioxidant and antibacterial activity. The pure compounds were isolated by chromatographic techniques. Structural determinations of the compounds were accomplished using a spectroscopic method, comprising 1D and 2D NMR. Twenty isolates of fungal endophyte were found residing in the stem bark of sungkai, specifically RB1-RB20. Isolates RB4 and RB6 showed the most potential antioxidant and antibacterial activity and were identified molecularly. The results of molecular identification showed that RB4 and RB6 were Curvularia intermedia and Colletotrichum cliviicola. Based on the spectroscopic analysis, the compounds identified from the two fungi were the different compound, specifically 3- hydroxy-4(hydroxy(4-hydroxyphenyl)methyl)-ɣ-butyrolactone (1) and 5-hydroxy-4-(hydroxymethyl)-2H-pyran-2-on (2). Compound 1 and 2 showed antibacterial and antioxidant in strong to moderate. These compounds and the endophytic fungi extract can be used as a new ingredient for medicine because it has antioxidant and antibacterial activity with further research.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Rian Oktiansyah This is me
0000-0002-4747-396X
Indonesia

Elfita Elfita ^*
0000-0003-2527-6639
Indonesia

Hary Wıdjajantı
0000-0001-5555-1537
Indonesia

Poedji Loekitowati Hariani This is me
0000-0002-3436-7199
Indonesia

Nurlisa Hidayati This is me
0000-0002-8306-6392
Indonesia

Arum Setıawan
0000-0001-6024-1090
Indonesia

Salni Salni This is me
0000-0002-5509-7917
Indonesia

Publication Date

June 28, 2025

Submission Date

April 30, 2023

Acceptance Date

September 7, 2023

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Year 2024 Volume: 28 Number: 1

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https://izlik.org/JA23CB78JS

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RIS / Bibtex

APA

Oktiansyah, R., Elfita, E., Wıdjajantı, H., Hariani, P. L., Hidayati, N., Setıawan, A., & Salni, S. (2025). Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.). Journal of Research in Pharmacy, 28(1), 89-109. https://izlik.org/JA23CB78JS

AMA

1.Oktiansyah R, Elfita E, Wıdjajantı H, et al. Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.). J. Res. Pharm. 2025;28(1):89-109. https://izlik.org/JA23CB78JS

Chicago

Oktiansyah, Rian, Elfita Elfita, Hary Wıdjajantı, et al. 2025. “Secondary Metabolites of Endophytic Fungii Isolated from the Stem Bark of Sungkai (Peronema Canescens Jack.)”. Journal of Research in Pharmacy 28 (1): 89-109. https://izlik.org/JA23CB78JS.

EndNote

Oktiansyah R, Elfita E, Wıdjajantı H, Hariani PL, Hidayati N, Setıawan A, Salni S (June 1, 2025) Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.). Journal of Research in Pharmacy 28 1 89–109.

IEEE

[1]R. Oktiansyah et al., “Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.)”, J. Res. Pharm., vol. 28, no. 1, pp. 89–109, June 2025, [Online]. Available: https://izlik.org/JA23CB78JS

ISNAD

Oktiansyah, Rian - Elfita, Elfita - Wıdjajantı, Hary - Hariani, Poedji Loekitowati - Hidayati, Nurlisa - Setıawan, Arum - Salni, Salni. “Secondary Metabolites of Endophytic Fungii Isolated from the Stem Bark of Sungkai (Peronema Canescens Jack.)”. Journal of Research in Pharmacy 28/1 (June 1, 2025): 89-109. https://izlik.org/JA23CB78JS.

JAMA

1.Oktiansyah R, Elfita E, Wıdjajantı H, Hariani PL, Hidayati N, Setıawan A, Salni S. Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.). J. Res. Pharm. 2025;28:89–109.

MLA

Oktiansyah, Rian, et al. “Secondary Metabolites of Endophytic Fungii Isolated from the Stem Bark of Sungkai (Peronema Canescens Jack.)”. Journal of Research in Pharmacy, vol. 28, no. 1, June 2025, pp. 89-109, https://izlik.org/JA23CB78JS.

Vancouver

1.Rian Oktiansyah, Elfita Elfita, Hary Wıdjajantı, Poedji Loekitowati Hariani, Nurlisa Hidayati, Arum Setıawan, Salni Salni. Secondary metabolites of endophytic fungii isolated from the stem bark of Sungkai (Peronema canescens Jack.). J. Res. Pharm. [Internet]. 2025 Jun. 1;28(1):89-109. Available from: https://izlik.org/JA23CB78JS