Comparative evaluation of anti-obesity effect through pancreatic lipase inhibition of methanolic extract of the bark of Saraca asoca and Cynometra travancorica

Pattilthodika Suhail; Parayil Varghese Christapher; Sheron Joseph; Nochikat Velayudhan Prasanth; Musliyarakath Nishida; Theruvath Anju; Smitha Rani

Comparative evaluation of anti-obesity effect through pancreatic lipase inhibition of methanolic extract of the bark of Saraca asoca and Cynometra travancorica

Abstract

Saraca Asoca, a member of the Caesalpiniaceae sub-family, is a native plant utilized extensively in ayurvedic medicine. This study compares the anti-obesity effect of methanolic extract of the bark of Saraca asoca and Cynometra travancorica, two common ingredients of many Indian traditional medicines. Plant material extracts were prepared using simple maceration technique. Adult 150-180 gm weighed Sprague dawley rats were used for high fat diet induced in vivo anti-obesity study for eight weeks. Weekly bodyweight was measured, and terminal serum lipid profile was estimated to assess the anti-obesity activity of standard drug orlistat (60 mg/kg) and extracts at a dose of 400 mg/kg. In vitro enzyme inhibition study was performed to assess the effect of standard or extracts on pancreatic lipase. Increase in bodyweight found corresponding with the normal weight gain for the control group whereas it is significantly increased with high fat diet fed group. While in the orlistat or extract treated found to have resisted significant changes in bodyweight (p<0.001). Terminal blood samples were collected from all animals and serum lipid profile were evaluated. An increase in the levels of TC, TG, and LDL of animals in high fat diet fed group when compared to normal control group. Whereas the increase lipid levels were reversed in all treated groups. HDL level found to have increased in standard and extract treated groups. In vitro enzyme inhibition study revealed the inhibitory potential of S. asoka (IC50 of 306.15µg/ml) and C. travancorica (IC50 of 301.94 µg/ml) on pancreatic lipase when compared with orlistat (IC50 of 262.17 µg/ml) (p<0.05). This study suggests that the methanolic extract of the bark of S. asoca and C. travancorica possess significant anti-obesity activity on high fat diet induced obesity rat model and in-vitro pancreatic lipase inhibition.

Keywords

References

[1] Wang YC, McPherson K, Marsh T, Gortmaker SL, Brown M. Health, and economic burden of the projected obesity trends in the USA and the UK. The Lancet. 2011; 378(9793): 815-825. https://doi.org/10.1016/S0140-6736(11)60814-3
[2] Billington CJ, Epstein LH, Goodwin NJ, Hill JO, Pi-Sunyer FX, Rolls BJ, Stern J, Wadden TA, Weinsier RL, Wilson GT, Wing RR, Yanovski SZ, Hubbard VS, Hoofnagle JH, Everhart J, Harrison B. Overweight, obesity, and health risk. Arch Intern Med. 2000; 160(7): 898-904. https://doi.org/10.1001/archinte.160.7.898
[3] Kopelman PG. Obesity as a medical problem. Nature. 2000; 404(6778): 635-643. https://doi.org/10.1038/35007508
[4] WHO Obesity and Overweight. http://www.who.int/mediacentre/factsheets/fs311/en/. (accessed February 28, 2023).
[5] Wei K, Wang GQ, Bai X, Niu YF, Chen HP, Wen CN, Li ZH, Dong ZJ, Zuo ZL, Xiong WY, Liu JK. Structure-based optimization and biological evaluation of pancreatic lipase inhibitors as novel potential antiobesity agents. Nat Prod Bioprospect. 2015; 5: 129-157. https://doi.org/10.1007/s13659-015-0062-6
[6] GBD 2015 Obesity Collaborators. Health effects of overweight and obesity in 195 countries over 25 years. N Engl J Med. 2017; 377(1): 13-27. https://doi.org/10.1056/nejmoa1614362
[7] Obata A, Okauchi S, Kimura T, Hirukawa H, Tanabe A, Kinoshita T, Kohara K, Tatsumi F, Shimoda M, Kamei S, Nakanishi S, Mune T, Kaku K, Kanate H. Advanced breast cancer in a relatively young man with severe obesity and type 2 diabetes mellitus. J Diabetes Investig. 2017; 8(3): 395-396. https://doi.org/10.1111/jdi.12570
[8] Seyedan A, Alshawsh MA, Alshagga MA, Koosha S, Mohamed Z. Medicinal plants and their inhibitory activities against pancreatic lipase: a review. Evid Based Complement Alternat Med. 2015; 2015: 973143. https://doi.org/10.1155/2015/973143

[9] Veeramachaneni GK, Raj KK, Chalasani LM, Annamraju SK, Bondili JS, Talluri VR. Shape based virtual screening and molecular docking towards designing novel pancreatic lipase inhibitors. Bioinformation. 2015; 11(12): 535–542. https://doi.org/10.6026/97320630011535
[10] Birari RB, Bhutani KK. Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discov Today. 2007; 12(19-20): 879-889. https://doi.org/10.1016/j.drudis.2007.07.024
[11] Ahn JH, Shin E, Liu Q, Kim SB, Choi KM, Yoo HS, Hwang BY, Lee MK. Secoiridoids from the stem barks of Fraxinus rhynchophylla with pancreatic lipase inhibitory activity. Nat Prod Res. 2013; 27(12): 1132-1135. https://doi.org/10.1080/14786419.2012.711328
[12] Bourne Y, Martinez C, Kerfelec B, Lombardo D, Chapus C, Cambillau C. Horse pancreatic lipase: the crystal structure refined at 2· 3 Å resolution. J Mol Biol.; 238(5): 709–732. https://doi.org/10.1006/jmbi.1994.1331
[13] Maury J, Issad T, Perdereau D, Gouhot B, Ferre P, Girard J. Effect of acarbose on glucose homeostasis, lipogenesis and lipogenic enzyme gene expression in adipose tissue of weaned rats. Diabetologia. 1993; 36(6): 503–509. https://doi.org/10.1007/bf02743265
[14] Hegde S, Hegde HV, Jalalpure SS, Peram MR, Pai SR, Roy S. Resolving identification issues of Saraca asoca from its adulterant and commercial samples using phytochemical markers. Pharmacogn Mag. 2017; 13(Suppl 2): S266. https://doi.org/10.4103/pm.pm_417_16
[15] Tusharkumar D. PhD. thesis. Chemical investigation of phenolic constituents of two important medicinal plants Terminalia chebula and Saraca asoca. Shri Jagdishprasad Jhabarmal Tibarewala University, Jhunjhunu, Rajasthan, India, 2011.
[16] Saha J, Mukherjee S, Gupta K, Gupta B. High-performance thin-layer chromatographic analysis of antioxidants present in different parts of Saraca asoca (Roxb.) de Wilde. J Pharm Res. 2013; 7(9): 798–803. https://doi.org/10.1016/j.jopr.2013.10.004
[17] Mathew N, Anitha MG, Bala TSL, Sivakumar SM, Narmadha R, Kalyanasundaram M. Larvicidal activity of Saraca indica, Nyctanthes arbor-tristis, and Clitoria ternatea extracts against three mosquito vector species. Parasitol Res. 2009; 104(5): 1017–1025. https://doi.org/10.1007/s00436-008-1284-x
[18] Cibin TR, Devi DG, Abraham A. Chemoprevention of two-stage skin cancer in vivo by Saraca asoca. Integr Cancer Ther. 2012; 11(3): 279–286. https://doi.org/10.1177/1534735411413264
[19] Kumar S, Narwal S, Kumar D, Singh G, Narwal S, Arya R. Evaluation of antihyperglycemic and antioxidant activities of Saraca asoca (Roxb.) De Wild leaves in streptozotocin induced diabetic mice. Asian Pacific J Trop Dis. 2012; 2(3): 170–176. https://doi.org/10.1016/S2222-1808(12)60041-3
[20] Sasmal S, Majumdar S, Gupta M, Mukherjee A, Mukherjee PK. Pharmacognostical, phytochemical and pharmacological evaluation for the antipyretic effect of the seeds of Saraca asoca Roxb. Asian Pac J Trop Biomed. 2012; 2(10): 782–786. https://doi.org/10.1016/S2221-1691(12)60229-9
[21] Shirolkar A, Gahlaut A, Chhillar AK, Dabur R. Quantitative analysis of catechins in Saraca asoca and correlation with antimicrobial activity. J Pharm Anal. 2013; 3(6): 421–428. https://doi.org/10.1016/j.jpha.2013.01.007
[22] Suhail PT, Joseph S, Ajeesh V, Sreelakshmi S, Anil K. Antiestrogenic and toxicological evaluation of methanolic extract of Saraca asoca and Cynometra travancorica. J Res Pharm. 2022; 26(5): 1261-1271. https://doi.org/10.29228/jrp.218
[23] Ahmad F, Misra L, Tewari R, Gupta P, Mishra P, Shukla R. Anti-inflammatory flavanol glycosides from Saraca asoca bark. Nat Prod Res. 2016; 30(4): 489-492. https://doi.org/10.1080/14786419.2015.1023728
[24] Sabiha S, Serrano R, Hasan K, da Silva IBM, Rocha J, Islam N, Silva O. The Genus Cynometra: A review of ethnomedicine, chemical, and biological data. Plants. 2022; 11(24): 3504. https://doi.org/10.3390/plants11243504
[25] Middelkoop TB, Labadie RP. The action of Saraca asoca Roxb. de Wilde bark on the PGH2 synthetase enzyme complex of the sheep vesicular gland. Z Naturforsch C Biosci. 1985; 40(7–8): 523–526. https://doi.org/10.1515/znc-1985-7-812
[26] Sasidharan N, Muraleedhara PK. Survey on the commercial exploitation and consumption of medicinal plants by the drug industry in Northern Kerala. Research Report No.193, ISBN 0970-8103, Kerala Forest Research Institute, Thrissur, Kerala 2000.
[27] Hrideek TK, Geethu PD, Jijeesh CM, Raghu AV, Muraleekrishnan K. Standardization of adventitious root induction in stem cuttings of Cynometra travancorica Bedd Willd., an endangered tree species of Western Ghats. Vegetos. 2019; 32: 11-18. https://doi.org/10.1007/s42535-019-00002-x
[28] Malakul W, Thirawarapan S, Suvitayavat W, Woodman OL. Type 1 diabetes and hypercholesterolaemia reveal the contribution of endothelium‐derived hyperpolarizing factor to endothelium‐dependent relaxation of the rat aorta. Clin Exp Pharmacol Physiol. 2008; 35(2): 192–200. https://doi.org/10.1111/j.1440-1681.2007.04811.x
[29] Martinez-Gonzalez AI, Alvarez-Parrilla E, Díaz-Sánchez ÁG, de la Rosa LA, Núñez-Gastélum JA, Vazquez-Flores AA, Gonzalez-Aguilar GA. In vitro inhibition of pancreatic lipase by polyphenols: A kinetic, fluorescence spectroscopy and molecular docking study. Food Technol Biotechnol 2017; 55(4): 519-530. https://doi.org/10.17113/ftb.55.04.17.5138
[30] Xu X, Pan J, Zhou X. Amelioration of lipid profile and level of antioxidant activities by epigallocatechin-gallate in a rat model of atherogenesis. Heart, Lung Circ. 2014; 23(12): 1194-201. https://doi.org/10.1016/j.hlc.2014.05.013
[31] Roberts KT. The potential of fenugreek (Trigonella foenum-graecum) as a functional food and nutraceutical and its effects on glycemia and lipidemia. J Med Food. 2011; 14(12): 1485-1489. https://doi.org/10.1089/jmf.2011.0002
[32] Mukherjee M. Human digestive and metabolic lipases—a brief review. J Mol Catal B Enzym. 2003; 22(5-6): 369-376. https://doi.org/10.1016/S1381-1177(03)00052-3
[33] Liu TT, Liu XT, Chen QX, Shi Y. Lipase inhibitors for obesity: A review. Biomed Pharmacother. 2020; 128: 110314. https://doi.org/10.1016/j.biopha.2020.110314
[34] Martins IJ, Redgrave TG. Obesity and post-prandial lipid metabolism. Feast or famine?. The J Nutr Biochem. 2004; 15(3): 130-141. https://doi.org/10.1016/j.jnutbio.2003.10.006
[35] Mbikay M. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: a review. Front Pharmacol. 2012; 3: 24. https://doi.org/10.3389/fphar.2012.00024
[36] Altunkaynak Z. Effects of high fat diet induced obesity on female rat livers (a histochemical study). Eur J Gen Med. 2005; 2(3): 100-109. https://doi.org/10.29333/ejgm/82319
[37] Bais S, Singh GS, Sharma R. Antiobesity and hypolipidemic activity of Moringa oleifera leaves against high fat diet-induced obesity in rats. Adv Biol. 2014; 2014: Article ID 162914. https://doi.org/10.1155/2014/162914

Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Pattilthodika Suhail ^*
India

Parayil Varghese Christapher This is me
India

Sheron Joseph This is me
India

Nochikat Velayudhan Prasanth This is me
India

Musliyarakath Nishida This is me
India

Theruvath Anju This is me
India

Smitha Rani This is me
India

Publication Date

June 28, 2025

Submission Date

March 3, 2023

Acceptance Date

June 1, 2023

Published in Issue

Year 2023 Volume: 27 Number: 6

IZ

https://izlik.org/JA92HP76EW

Cite

RIS / Bibtex

APA

Suhail, P., Christapher, P. V., Joseph, S., Prasanth, N. V., Nishida, M., Anju, T., & Rani, S. (2025). Comparative evaluation of anti-obesity effect through pancreatic lipase inhibition of methanolic extract of the bark of Saraca asoca and Cynometra travancorica. Journal of Research in Pharmacy, 27(6), 2463-2470. https://izlik.org/JA92HP76EW

AMA

1.Suhail P, Christapher PV, Joseph S, et al. Comparative evaluation of anti-obesity effect through pancreatic lipase inhibition of methanolic extract of the bark of Saraca asoca and Cynometra travancorica. J. Res. Pharm. 2025;27(6):2463-2470. https://izlik.org/JA92HP76EW

Chicago

Suhail, Pattilthodika, Parayil Varghese Christapher, Sheron Joseph, et al. 2025. “Comparative Evaluation of Anti-Obesity Effect through Pancreatic Lipase Inhibition of Methanolic Extract of the Bark of Saraca Asoca and Cynometra Travancorica”. Journal of Research in Pharmacy 27 (6): 2463-70. https://izlik.org/JA92HP76EW.

EndNote

Suhail P, Christapher PV, Joseph S, Prasanth NV, Nishida M, Anju T, Rani S (July 1, 2025) Comparative evaluation of anti-obesity effect through pancreatic lipase inhibition of methanolic extract of the bark of Saraca asoca and Cynometra travancorica. Journal of Research in Pharmacy 27 6 2463–2470.

IEEE

[1]P. Suhail et al., “Comparative evaluation of anti-obesity effect through pancreatic lipase inhibition of methanolic extract of the bark of Saraca asoca and Cynometra travancorica”, J. Res. Pharm., vol. 27, no. 6, pp. 2463–2470, July 2025, [Online]. Available: https://izlik.org/JA92HP76EW

ISNAD

Suhail, Pattilthodika - Christapher, Parayil Varghese - Joseph, Sheron - Prasanth, Nochikat Velayudhan - Nishida, Musliyarakath - Anju, Theruvath - Rani, Smitha. “Comparative Evaluation of Anti-Obesity Effect through Pancreatic Lipase Inhibition of Methanolic Extract of the Bark of Saraca Asoca and Cynometra Travancorica”. Journal of Research in Pharmacy 27/6 (July 1, 2025): 2463-2470. https://izlik.org/JA92HP76EW.

JAMA

1.Suhail P, Christapher PV, Joseph S, Prasanth NV, Nishida M, Anju T, Rani S. Comparative evaluation of anti-obesity effect through pancreatic lipase inhibition of methanolic extract of the bark of Saraca asoca and Cynometra travancorica. J. Res. Pharm. 2025;27:2463–2470.

MLA

Suhail, Pattilthodika, et al. “Comparative Evaluation of Anti-Obesity Effect through Pancreatic Lipase Inhibition of Methanolic Extract of the Bark of Saraca Asoca and Cynometra Travancorica”. Journal of Research in Pharmacy, vol. 27, no. 6, July 2025, pp. 2463-70, https://izlik.org/JA92HP76EW.

Vancouver

1.Pattilthodika Suhail, Parayil Varghese Christapher, Sheron Joseph, Nochikat Velayudhan Prasanth, Musliyarakath Nishida, Theruvath Anju, Smitha Rani. Comparative evaluation of anti-obesity effect through pancreatic lipase inhibition of methanolic extract of the bark of Saraca asoca and Cynometra travancorica. J. Res. Pharm. [Internet]. 2025 Jul. 1;27(6):2463-70. Available from: https://izlik.org/JA92HP76EW